BB 451 Exam 4 questions well answered
to pass graded A+
Which of the following is not a function of DNA Polymerase III?
Clamping onto the template
Polymerization
Ligating the final products
Proofreading - correct answer ✔✔Ligating the final products
An important process in the synthesis of new DNA is:
Unwinding of the double helix
All of these.
Proofreading and repair
Protection of single-stranded regions from nuclease action - correct answer ✔✔All of these.
The mechanism for all template-directed synthesis of any type of nucleic acid involves the
following:
Nucleophilic attack of a 5' hydroxyl toward a nucleoside triphosphate, releasing PPi.
Nucleophilic attack of a 5' hydroxyl toward a nucleoside triphosphate, releasing Pi.
Nucleophilic attack of a 3' hydroxyl toward a nucleoside triphosphate, releasing Pi.
Nucleophilic attack of a 3' hydroxyl toward a nucleoside triphosphate, releasing PPi. - correct
answer ✔✔Nucleophilic attack of a 3' hydroxyl toward a nucleoside triphosphate, releasing PPi.
, Bulky DNA adducts or thymine dimers would be repaired by:
Mismatch repair
Nucleotide excision repair
Base excision repair - correct answer ✔✔Nucleotide excision repair
The backbone of nucleic acids consists of a:
Glycosidic bond between a purine and a sugar
Glycosidic bond between a pyrimidine and a sugar
Phosphodiester bond between the 2' and 5' hydroxyl groups of neighboring sugars
Phosphodiester bond between the 3' and 5' hydroxyl groups of neighboring sugars - correct
answer ✔✔Phosphodiester bond between the 3' and 5' hydroxyl groups of neighboring sugars
Why is DNA replication called "semi-conservative"?
The new daughter cells each end up with one of the original parent strands
There is no guarantee all the right bases will be added to the new strand - correct answer
✔✔The new daughter cells each end up with one of the original parent strands
Methylation of A's on the DNA strand, to indicate which strand is original, is a major mechanism
recognized by which type of DNA repair?
Mismatch repair
Base excision repair
Nucleotide excision repair - correct answer ✔✔Mismatch repair
to pass graded A+
Which of the following is not a function of DNA Polymerase III?
Clamping onto the template
Polymerization
Ligating the final products
Proofreading - correct answer ✔✔Ligating the final products
An important process in the synthesis of new DNA is:
Unwinding of the double helix
All of these.
Proofreading and repair
Protection of single-stranded regions from nuclease action - correct answer ✔✔All of these.
The mechanism for all template-directed synthesis of any type of nucleic acid involves the
following:
Nucleophilic attack of a 5' hydroxyl toward a nucleoside triphosphate, releasing PPi.
Nucleophilic attack of a 5' hydroxyl toward a nucleoside triphosphate, releasing Pi.
Nucleophilic attack of a 3' hydroxyl toward a nucleoside triphosphate, releasing Pi.
Nucleophilic attack of a 3' hydroxyl toward a nucleoside triphosphate, releasing PPi. - correct
answer ✔✔Nucleophilic attack of a 3' hydroxyl toward a nucleoside triphosphate, releasing PPi.
, Bulky DNA adducts or thymine dimers would be repaired by:
Mismatch repair
Nucleotide excision repair
Base excision repair - correct answer ✔✔Nucleotide excision repair
The backbone of nucleic acids consists of a:
Glycosidic bond between a purine and a sugar
Glycosidic bond between a pyrimidine and a sugar
Phosphodiester bond between the 2' and 5' hydroxyl groups of neighboring sugars
Phosphodiester bond between the 3' and 5' hydroxyl groups of neighboring sugars - correct
answer ✔✔Phosphodiester bond between the 3' and 5' hydroxyl groups of neighboring sugars
Why is DNA replication called "semi-conservative"?
The new daughter cells each end up with one of the original parent strands
There is no guarantee all the right bases will be added to the new strand - correct answer
✔✔The new daughter cells each end up with one of the original parent strands
Methylation of A's on the DNA strand, to indicate which strand is original, is a major mechanism
recognized by which type of DNA repair?
Mismatch repair
Base excision repair
Nucleotide excision repair - correct answer ✔✔Mismatch repair
