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BIOC405 LECTURE 25 EXAM QUESTIONS AND ANSWERS WITH VERIFIED SOLUTIONS

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BIOC405 LECTURE 25 EXAM QUESTIONS AND ANSWERS WITH VERIFIED SOLUTIONS What is amylose? amylopectin? amylose: glucose linear chain of α(1-4) linkages amylopectin: same as amylose but also has a α(1-6) branch every 24-30 subunits what is starch? what is it comprised of? plants store glucose as starch starch is comprised of glucose polymers in the form of amylose and amylopectin Why does glycogen phosphorylase have a limited reach? there is a groove in the enzyme that binds 4 monosaccharide units, but the active site is after those 4 that it's bound to how do amylases give yeast a boost in bread dough? if you add amylase in bread w/ yeast, the amylase will break down the starch (flour) faster, feed it to the yeast, and they'll make bubbles faster and get fluffier faster glucose and maltose are readily used by yeast to make ____ CO2 is amylose more branched in humans or plants? how much more branched? amylose is much more branched in humans (every 8-14 units) than in plants (20-24 units) what are glycogen granules? Mini compartments containing 20-40 glycogen molecules and enzymes for glycogen synthesis and degradation what are differences between storing energy as fat vs glycogen? fat: - storing the fat is for much longer term bc it is much slower to build and slower to break down glycogen: - synthesis and breakdown is rapid (think of it as a battery) - helps maintain blood glucose level when fasting glycogen degradation and synthesis occur @ ______ non-reducing ends - large number of non-reducing ends provide lots of glucose rapidly Describe the role of glycogen during exercise or fight/flight response when doing exercise (escaping aaron donald) you want to rapidly break down the glycogen that's in your muscles to rapidly release that glucose and get energy out of it - there is so much glucose per glycogen molecule that it takes minutes/hours before you start running out where is glycogen stored? What are properties of them? liver - glycogen makes up to 10% net weight of liver (very dense in glycogen) - exportable glucose reservoir - exhausted in 12-24 hrs skeletal muscle - glycogen makes up 1-2% of weight (not very dense, but since we have so much skeletal muscle, most of our glycogen comes from there) - usable energy reservoir - exhauster in ~1 hr what are the steps in glygenolysis? 1. glycogen phosphorylase cleaves α(1-4) linkages from non-reducing ends until it reaches 4 units from a branch point. It sticks a free phosphate group (not from ATP) onto cleaved glucose to form G1P

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BIOC405 LECTURE 25 EXAM QUESTIONS AND ANSWERS

WITH VERIFIED SOLUTIONS


What is amylose? amylopectin?

amylose: glucose linear chain of α(1->4) linkages

amylopectin: same as amylose but also has a α(1->6) branch every 24-30 subunits

what is starch? what is it comprised of?

plants store glucose as starch

starch is comprised of glucose polymers in the form of amylose and amylopectin

Why does glycogen phosphorylase have a limited reach?

there is a groove in the enzyme that binds 4 monosaccharide units, but the active site is

after those 4 that it's bound to

how do amylases give yeast a boost in bread dough?

if you add amylase in bread w/ yeast, the amylase will break down the starch (flour)

faster, feed it to the yeast, and they'll make bubbles faster and get fluffier faster

glucose and maltose are readily used by yeast to make ____

CO2

is amylose more branched in humans or plants? how much more branched?

amylose is much more branched in humans (every 8-14 units) than in plants (20-24

units)

what are glycogen granules?

, Mini compartments containing 20-40 glycogen molecules and enzymes for glycogen

synthesis and degradation

what are differences between storing energy as fat vs glycogen?

fat:

- storing the fat is for much longer term bc it is much slower to build and slower to break

down

glycogen:

- synthesis and breakdown is rapid (think of it as a battery)

- helps maintain blood glucose level when fasting

glycogen degradation and synthesis occur @ ______

non-reducing ends

- large number of non-reducing ends provide lots of glucose rapidly

Describe the role of glycogen during exercise or fight/flight response

when doing exercise (escaping aaron donald) you want to rapidly break down the

glycogen that's in your muscles to rapidly release that glucose and get energy out of it

- there is so much glucose per glycogen molecule that it takes minutes/hours before you

start running out

where is glycogen stored? What are properties of them?

liver

- glycogen makes up to 10% net weight of liver (very dense in glycogen)

- exportable glucose reservoir

- exhausted in 12-24 hrs

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