BIOC0005 PART 1 EXAM QUESTIONS AND ANSWERS
WITH VERIFIED SOLUTIONS GRADED A++
Cloning vector
DNA section allowing insertion of external DNA for cloning
Plasmid vectors
Artificial DNA molecules with replication origin, marker, insertion site
Expression vectors
Vectors allowing replication and expression of inserted DNA
Promoter
Region initiating gene transcription
Terminator
Sequence ending gene transcription
Reporter genes
Genes used to visualize regulatory elements' strength
Lambda genome
Phage genome with lytic and lysogenic cycles used as a vector
Cosmid vectors
Plasmids with cos sites for lambda packaging
Fosmids
Cosmids with low copy number to counteract instability
Bacterial artificial chromosome (BAC)
, Plasmid vectors with elements for controlled replication
Yeast artificial chromosome (YAC)
Vectors for chromosome maintenance and replication in yeast
DNA sequencing approaches
Biological, chemical, or physical methods to determine base order
Sanger sequencing
Biological method using DNA polymerase and dideoxynucleotides
Next generation sequencing
Sequencing method for larger DNA amounts, e.g., nanopore sequencing
Recombinant expression
Technique involving the expression of foreign genes in host cells
Metabolic engineering
Optimizing cellular processes for bio-based production
Plasmid replication
Autonomous DNA replication in plasmids
Incompatibility groups
Plasmid groups leading to loss of non-essential plasmids
Plasmid partitioning
Random process distributing plasmids into daughter cells
ParR and ParM
Proteins involved in plasmid partitioning via filament formation
Plasmid R1
Uses actin-like filament to push plasmids apart in cell
WITH VERIFIED SOLUTIONS GRADED A++
Cloning vector
DNA section allowing insertion of external DNA for cloning
Plasmid vectors
Artificial DNA molecules with replication origin, marker, insertion site
Expression vectors
Vectors allowing replication and expression of inserted DNA
Promoter
Region initiating gene transcription
Terminator
Sequence ending gene transcription
Reporter genes
Genes used to visualize regulatory elements' strength
Lambda genome
Phage genome with lytic and lysogenic cycles used as a vector
Cosmid vectors
Plasmids with cos sites for lambda packaging
Fosmids
Cosmids with low copy number to counteract instability
Bacterial artificial chromosome (BAC)
, Plasmid vectors with elements for controlled replication
Yeast artificial chromosome (YAC)
Vectors for chromosome maintenance and replication in yeast
DNA sequencing approaches
Biological, chemical, or physical methods to determine base order
Sanger sequencing
Biological method using DNA polymerase and dideoxynucleotides
Next generation sequencing
Sequencing method for larger DNA amounts, e.g., nanopore sequencing
Recombinant expression
Technique involving the expression of foreign genes in host cells
Metabolic engineering
Optimizing cellular processes for bio-based production
Plasmid replication
Autonomous DNA replication in plasmids
Incompatibility groups
Plasmid groups leading to loss of non-essential plasmids
Plasmid partitioning
Random process distributing plasmids into daughter cells
ParR and ParM
Proteins involved in plasmid partitioning via filament formation
Plasmid R1
Uses actin-like filament to push plasmids apart in cell
