BIOC0007 EXAM QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED
BRE
TFIIB recognition element
INR
Initiator element
Sigma 70
Recognises promoter and secures RNA binding
Sigma 1
Prevents premature dissociation
Sigma 2
Separates dsDNA and stabilises ssDNA
3.2 region
Forms loop between sigma 3 and 4 and helps position incoming dNTPs
Preinitiation complex in vitro
TBP+TATA; D-TBP binds INR and DPE; A; B binds BRE; F; E; H
Termination in prokaryotes: Intrinsic
RNA hairpin- loop with a string of 8-10U bases pairs with transcribed poly-U in the
transcription bubble
Two factors of Allosteric termination in eukaryotes
, CPSF, CSTF
DPE
Downstream Promoter Element
Additional factors recruited to the RNA in allosteric termination in eukaryotes
PAP(use ATP as source of adenine)
RNA capping
3 phosphates at 5' end; triphosphate removes gammaP; GMP added (5' to 5' linkage);
Guanine methylated—> cap created
5' splice site
GU
3' splice site
AG
Splicing machinery
5' -U1, BBP binds at brunch point nucleotide, 3' - U2A35; U4-U6; pyrimide tract
recognised by U2A65; transesterification 1; intron lariat; transesterification 2
Polyadenylation
3' exonuclease cleavage; addition of 200As by PAP, PABP
Which ribosomal bonds are seen very clearly signifying hood quality of RNA?
28S and 18S
PABP
Protects tail from degradation
Membrane used northern blotting
Nitrocellulose
COMPLETE SOLUTIONS VERIFIED
BRE
TFIIB recognition element
INR
Initiator element
Sigma 70
Recognises promoter and secures RNA binding
Sigma 1
Prevents premature dissociation
Sigma 2
Separates dsDNA and stabilises ssDNA
3.2 region
Forms loop between sigma 3 and 4 and helps position incoming dNTPs
Preinitiation complex in vitro
TBP+TATA; D-TBP binds INR and DPE; A; B binds BRE; F; E; H
Termination in prokaryotes: Intrinsic
RNA hairpin- loop with a string of 8-10U bases pairs with transcribed poly-U in the
transcription bubble
Two factors of Allosteric termination in eukaryotes
, CPSF, CSTF
DPE
Downstream Promoter Element
Additional factors recruited to the RNA in allosteric termination in eukaryotes
PAP(use ATP as source of adenine)
RNA capping
3 phosphates at 5' end; triphosphate removes gammaP; GMP added (5' to 5' linkage);
Guanine methylated—> cap created
5' splice site
GU
3' splice site
AG
Splicing machinery
5' -U1, BBP binds at brunch point nucleotide, 3' - U2A35; U4-U6; pyrimide tract
recognised by U2A65; transesterification 1; intron lariat; transesterification 2
Polyadenylation
3' exonuclease cleavage; addition of 200As by PAP, PABP
Which ribosomal bonds are seen very clearly signifying hood quality of RNA?
28S and 18S
PABP
Protects tail from degradation
Membrane used northern blotting
Nitrocellulose
