Liver Function Tests
(LFT)
a.k.a Hepatic Panel (test by blood)
Liver is involved in most of the
metabolisms of body.
It has role in hemoglobin, protein,
lipid, carbohydrates and xenobiotics
metabolisms.
So they are affected in liver diseases.
By measuring various products of
these metabolisms in blood liver
function is assessed. PROTEIN METABOLISM
These functional tests mostly
assesses synthetic function of
HEMOGLOBIN (BILE PIGMENT) liver because many proteins, urea
METABOLISM and ammonia are handled by liver
Serum Bilirubin Serum albumin:
Secretory function of liver is Since liver synthesizes albumin low
assessed by measuring serum albumin level indicates liver
bilirubin. disease like cirrhosis.
Van den Bergh Test: Serum globulins:
It is used to measure conjugated and Increased globulin level occurs in
unconjugated bilirubin level in blood. blood in liver disease like cirrhosis,
auto immune hepatitis, biliary
a. Conjugated bilirubin: (Direct cirrhosis etc.
Positive) Method: Biuret method is used to
It is more in obstructive jaundice. estimate proteins in serum
b. Unconjugated bilirubin: (Indirect
Positive)
In pre hepatic jaundice it is more.
(aka Hemolytic Jaundice)
c. Conjugated and unconjugated
bilirubin:
In hepatic jaundice both are
elevated.
Urine bilirubin:
In normal people urine bilirubin Proteins are polymers of amino
is absent. It is found in urine in post acids joined together by covalent
hepatic or obstructive type jaundice. peptide bonds. Biurets solution
Fouchet's test is used for urine reacts with peptide bonds to give a
bilirubin. violet color.
Urine urobilinogen: Single peptide bonds occur between
Urobilinogen in urine increases in each amino acid pairing. Proteins are
pre hepatic jaundice. also called polypeptides as they are
Decrease in urine urobilinogen often made up of tens and even
occurs in post hepatic or obstructive hundreds of amino acids that have
jaundice. been joined together into peptide
Ehrlich's test detects urobilinogen chains. This means that proteins
in urine. contain many peptide bonds.
Prothrombin time (PT):
(LFT)
a.k.a Hepatic Panel (test by blood)
Liver is involved in most of the
metabolisms of body.
It has role in hemoglobin, protein,
lipid, carbohydrates and xenobiotics
metabolisms.
So they are affected in liver diseases.
By measuring various products of
these metabolisms in blood liver
function is assessed. PROTEIN METABOLISM
These functional tests mostly
assesses synthetic function of
HEMOGLOBIN (BILE PIGMENT) liver because many proteins, urea
METABOLISM and ammonia are handled by liver
Serum Bilirubin Serum albumin:
Secretory function of liver is Since liver synthesizes albumin low
assessed by measuring serum albumin level indicates liver
bilirubin. disease like cirrhosis.
Van den Bergh Test: Serum globulins:
It is used to measure conjugated and Increased globulin level occurs in
unconjugated bilirubin level in blood. blood in liver disease like cirrhosis,
auto immune hepatitis, biliary
a. Conjugated bilirubin: (Direct cirrhosis etc.
Positive) Method: Biuret method is used to
It is more in obstructive jaundice. estimate proteins in serum
b. Unconjugated bilirubin: (Indirect
Positive)
In pre hepatic jaundice it is more.
(aka Hemolytic Jaundice)
c. Conjugated and unconjugated
bilirubin:
In hepatic jaundice both are
elevated.
Urine bilirubin:
In normal people urine bilirubin Proteins are polymers of amino
is absent. It is found in urine in post acids joined together by covalent
hepatic or obstructive type jaundice. peptide bonds. Biurets solution
Fouchet's test is used for urine reacts with peptide bonds to give a
bilirubin. violet color.
Urine urobilinogen: Single peptide bonds occur between
Urobilinogen in urine increases in each amino acid pairing. Proteins are
pre hepatic jaundice. also called polypeptides as they are
Decrease in urine urobilinogen often made up of tens and even
occurs in post hepatic or obstructive hundreds of amino acids that have
jaundice. been joined together into peptide
Ehrlich's test detects urobilinogen chains. This means that proteins
in urine. contain many peptide bonds.
Prothrombin time (PT):
