BIOL3204 - LIGHT MICROSCOPY EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE

BIOL3204 - LIGHT MICROSCOPY EXAM QUESTIONS AND

ANSWERS WITH COMPLETE SOLUTIONS VERIFIED

LATEST UPDATE


What is the main advantage of using light microscopy over electron microscopy?

imaging of living, moving, realtime cells/cellular activity

What is the basic need of microscopy?

to look at very small things --> i.e. things smaller that sizes capable of being seen by the

naked, human eye

To what size can the unaided human eye see? i.e. human eye can see items of

this size or bigger...

a) 200 nm

b) 200 μm

c) 0.2 μm

d) 0.2 mm

e) more than one of these (be specific)

e) more than one of these

--> b) 200 μm

--> d) 0.2 mm

To what size can a simple light microscopes see?

a) 200 nm

, b) 0.2 nm

c) 0.2 mm

d) 20 nm

a) 200 nm

To what size(s) can a super-resolution light microscopes see?

a) 200 nm

b) 10 nm

c) 20 nm

d) 0.2 nm

e) more than one of these (be specific)

e) more than one of these

--> b) 10 nm

--> c) 20 nm

between ~10-20 nm

To what size can an electron microscope see?

a) 5 nm

b) 0.2 nm

c) 0.2 μm

d) 0.2 mm

b) 0.2 nm

T/F: Light microscopes can be used to see organelles, subcellular nuclei,

mitochondria in living eukaryotic cells & most bacteria?

True