MBIO 3410 (Molecular Biology) Exam
Questions With Reviewed 100% Correct
Detailed Answers >> Guaranteed Pass!!
Latest Updated Rated A+
Cis elements - Answers - ✔✔on molecule to be regulated (such as origins of replication,
promoters)
Tran’s elements - Answers - ✔✔regulatory elements that bind to cis elements but are
not covalently bound to DNA/RNA (respond to conditions)
Annotation (in genomics) - Answers - ✔✔computer algorithms provide best guess for
function of ORFs
Transcriptomics - Answers - ✔✔all RNA in a cell at a given time in a given cell type
under specific conditions (includes non-coding RNA)
What does an ORF need to be considered real? - Answers - ✔✔100 AAs or 300 bp long
Epigenetics - Answers - ✔✔modifications to DNA that are inherited and influence gene
expression in offspring
Function of snRNA (eukaryotic) - Answers - ✔✔RNA splicing (only in nucleus)
Function of snoRNA (eukaryotic) - Answers - ✔✔RNA modification in nucleolus
Function of siRNA/miRNA (eukaryotic) - Answers - ✔✔regulatory/silencing, bp with
mRNA to block translation or target it for destruction
Ln RNAs (eukaryotic) - Answers - ✔✔not well understood, can bind and sequester
regulatory elements or bind and assist regulatory elements
Function of tm RNA (some bacterial mito) - Answers - ✔✔bind ribosome like RNA but
then ribosome uses them like mRNA, releases stalled ribosomes
Function of vtRNA (vault RNA eukaryotic) - Answers - ✔✔short RNA in a
ribonucleoprotein particle, may be involved in blocking apoptosis
Function of gRNA (guide RNA, eukaryotic) - Answers - ✔✔roles in telomere formation,
only in lower eukaryotic mito where mt DNA in incomplete
, Function of catalytic RNAs - Answers - ✔✔self-splicing introns, cis and Trans cleaving
ribosomes
processivity (property of DNA replication) - Answers - ✔✔number of nucleotides added
before polymerase dissociates (how far)
Fidelity (property of DNA replication) - Answers - ✔✔accuracy of replication, replicative
and repair
DNA pol I - Answers - ✔✔very abundant, main role is to remove Okazaki fragments
DNA pol II - Answers - ✔✔not in all bacteria, involved in repair
DNA pol III - Answers - ✔✔replicative enzyme, processive
DNA pols IV and V - Answers - ✔✔error prone repair enzymes
What do DNA pols I, II and III have - Answers - ✔✔polymerization and proofreading
activity (3'-5' exonuclease)
what extra activity does DNA pol I have - Answers - ✔✔5'-3' exonuclease activity
(degrades RNA primer and adds new nucleotides to previous DNA strand)
how do tmRNAs rescue stalled ribosomes - Answers - ✔✔catalyzes: addition of a non-
encoded alanine, ribosome switches to the mRNA like domain of tmRNA, thus resuming
protein synthesis adding a degradation signal
topoisomerase - Answers - ✔✔removes the overwinding generated by helicase by
breaking DNA and unwinding it and religating it
which DNA pol removes Okazaki fragments and fills gap - Answers - ✔✔DNA pol I
function of DNA ligase - Answers - ✔✔creates last phosphodiester bond to make a
continuous strand
RNA polymerase - Answers - ✔✔doesn't need need primer or separate helicase
what do cis elements in and near the promoter determine - Answers - ✔✔when and how
efficient transcription is
RNA polymerase - Answers - ✔✔can unwind DNA and doesn't need a primer
transition mutation - Answers - ✔✔purine replaced with purine
Questions With Reviewed 100% Correct
Detailed Answers >> Guaranteed Pass!!
Latest Updated Rated A+
Cis elements - Answers - ✔✔on molecule to be regulated (such as origins of replication,
promoters)
Tran’s elements - Answers - ✔✔regulatory elements that bind to cis elements but are
not covalently bound to DNA/RNA (respond to conditions)
Annotation (in genomics) - Answers - ✔✔computer algorithms provide best guess for
function of ORFs
Transcriptomics - Answers - ✔✔all RNA in a cell at a given time in a given cell type
under specific conditions (includes non-coding RNA)
What does an ORF need to be considered real? - Answers - ✔✔100 AAs or 300 bp long
Epigenetics - Answers - ✔✔modifications to DNA that are inherited and influence gene
expression in offspring
Function of snRNA (eukaryotic) - Answers - ✔✔RNA splicing (only in nucleus)
Function of snoRNA (eukaryotic) - Answers - ✔✔RNA modification in nucleolus
Function of siRNA/miRNA (eukaryotic) - Answers - ✔✔regulatory/silencing, bp with
mRNA to block translation or target it for destruction
Ln RNAs (eukaryotic) - Answers - ✔✔not well understood, can bind and sequester
regulatory elements or bind and assist regulatory elements
Function of tm RNA (some bacterial mito) - Answers - ✔✔bind ribosome like RNA but
then ribosome uses them like mRNA, releases stalled ribosomes
Function of vtRNA (vault RNA eukaryotic) - Answers - ✔✔short RNA in a
ribonucleoprotein particle, may be involved in blocking apoptosis
Function of gRNA (guide RNA, eukaryotic) - Answers - ✔✔roles in telomere formation,
only in lower eukaryotic mito where mt DNA in incomplete
, Function of catalytic RNAs - Answers - ✔✔self-splicing introns, cis and Trans cleaving
ribosomes
processivity (property of DNA replication) - Answers - ✔✔number of nucleotides added
before polymerase dissociates (how far)
Fidelity (property of DNA replication) - Answers - ✔✔accuracy of replication, replicative
and repair
DNA pol I - Answers - ✔✔very abundant, main role is to remove Okazaki fragments
DNA pol II - Answers - ✔✔not in all bacteria, involved in repair
DNA pol III - Answers - ✔✔replicative enzyme, processive
DNA pols IV and V - Answers - ✔✔error prone repair enzymes
What do DNA pols I, II and III have - Answers - ✔✔polymerization and proofreading
activity (3'-5' exonuclease)
what extra activity does DNA pol I have - Answers - ✔✔5'-3' exonuclease activity
(degrades RNA primer and adds new nucleotides to previous DNA strand)
how do tmRNAs rescue stalled ribosomes - Answers - ✔✔catalyzes: addition of a non-
encoded alanine, ribosome switches to the mRNA like domain of tmRNA, thus resuming
protein synthesis adding a degradation signal
topoisomerase - Answers - ✔✔removes the overwinding generated by helicase by
breaking DNA and unwinding it and religating it
which DNA pol removes Okazaki fragments and fills gap - Answers - ✔✔DNA pol I
function of DNA ligase - Answers - ✔✔creates last phosphodiester bond to make a
continuous strand
RNA polymerase - Answers - ✔✔doesn't need need primer or separate helicase
what do cis elements in and near the promoter determine - Answers - ✔✔when and how
efficient transcription is
RNA polymerase - Answers - ✔✔can unwind DNA and doesn't need a primer
transition mutation - Answers - ✔✔purine replaced with purine
