Test Bank For Thompson & Thompson Genetics and Genomics in
Medicine (Thompson and Thompson Genetics in Medicine) 9th Edition
by Ronald Cohn
Online Supplemental Material
Test Bank
MULTIPLE CHOICE
1. How did the development of bacterial artificial chromosomes (BACs) facilitate the
sequencing of the human genome?
A. It allowed human DNA to be cloned into bacteria for the first time
B. It allowed larger pieces of human DNA to be cloned
C. It allowed human chromosomes to be grown in bacteria
D. It allowed recombinants between human and bacteria to be made
E. It allowed recombination between human and bacterial chromosomes
ANS: B
2. Which of the following techniques is used to examine proteins?
A. Northern blot
B. Southern blot
C. Western blot
D. Microarray
E. All of the above
ANS: C
3. The generation of complementary DNA (cDNA) libraries requires which enzyme?
A. Reverse transcriptase
B. DNA polymerase
C. RNA polymerase
D. Polyadenylic (poly-A) polymerase
E. Exonuclease
ANS: A
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,Test Bank Online Supplemental Material-2
4. A Southern blot detects which of the following?
A. Alternative splicing
B. Point mutations
C. Transcriptional start sites
D. Large deletions
E. Frameshift mutations
ANS: D
5. Allele specific oligonucleotides are most useful for detection of what kind of mutations?
A. Small insertions
B. Common point mutations for a specific disease
C. Rare mutations
D. Frameshift mutations
E. Splice site mutations in a particular exon
ANS: B
6. This technique allows the amplification of DNA sequences:
A. Polymerase chain reaction (PCR)
B. Cloning into bacteria
C. Incorporation of dideoxynucleotides
D. A and B
E. A and C
ANS: D
7. Which of the following techniques detects a translocation?
A. Southern blot
B. PCR
C. Spectral karyotyping
D. Microarray
E. Comparative genome hybridization
ANS: C
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,Test Bank Online Supplemental Material-3
8. Comparative genome hybridization detects what type of change in the DNA?
A. Point mutations
B. Frameshift mutations
C. Alternative splicing
D. Dosage changes
E. Recombination
ANS: D
9. Which of the following techniques is used to measure the relative expression of many
transcripts simultaneously?
A. Microarray
B. Northern blot
C. Reverse transcriptase polymerase chain reaction (RT-PCR)
D. cDNA cloning
E. Comparative genome hybridization
ANS: A
10. What specifies the DNA sequence amplified in a PCR reaction?
A. The promoter and stop codon
B. The splice sites
C. The primers
D. DNA polymerase
E. The physical ends of the DNA
ANS: C
11. Which of the following techniques allows staining a particular chromosome specifically?
A. Comparative genome hybridization
B. Southern blot
C. G-banding
D. Fluorescent in situ hybridization (FISH)
E. Karyotyping
ANS: D
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, Test Bank Online Supplemental Material-4
12. Restriction enzyme digestion would NOT be used for which of the following techniques?
A. Restriction fragment length polymorphism (RFLP)
B. Cloning a PCR fragment
C. Southern blot
D. Northern blot
E. Construction of a BAC library
ANS: D
13. A probe is required for which of the following?
A. FISH
B. Northern blot
C. Southern blot
D. Western blot
E. All of the above
ANS: E
Copyright © . All rights reserved.
Medicine (Thompson and Thompson Genetics in Medicine) 9th Edition
by Ronald Cohn
Online Supplemental Material
Test Bank
MULTIPLE CHOICE
1. How did the development of bacterial artificial chromosomes (BACs) facilitate the
sequencing of the human genome?
A. It allowed human DNA to be cloned into bacteria for the first time
B. It allowed larger pieces of human DNA to be cloned
C. It allowed human chromosomes to be grown in bacteria
D. It allowed recombinants between human and bacteria to be made
E. It allowed recombination between human and bacterial chromosomes
ANS: B
2. Which of the following techniques is used to examine proteins?
A. Northern blot
B. Southern blot
C. Western blot
D. Microarray
E. All of the above
ANS: C
3. The generation of complementary DNA (cDNA) libraries requires which enzyme?
A. Reverse transcriptase
B. DNA polymerase
C. RNA polymerase
D. Polyadenylic (poly-A) polymerase
E. Exonuclease
ANS: A
Copyright © . All rights reserved.
,Test Bank Online Supplemental Material-2
4. A Southern blot detects which of the following?
A. Alternative splicing
B. Point mutations
C. Transcriptional start sites
D. Large deletions
E. Frameshift mutations
ANS: D
5. Allele specific oligonucleotides are most useful for detection of what kind of mutations?
A. Small insertions
B. Common point mutations for a specific disease
C. Rare mutations
D. Frameshift mutations
E. Splice site mutations in a particular exon
ANS: B
6. This technique allows the amplification of DNA sequences:
A. Polymerase chain reaction (PCR)
B. Cloning into bacteria
C. Incorporation of dideoxynucleotides
D. A and B
E. A and C
ANS: D
7. Which of the following techniques detects a translocation?
A. Southern blot
B. PCR
C. Spectral karyotyping
D. Microarray
E. Comparative genome hybridization
ANS: C
Copyright © . All rights reserved.
,Test Bank Online Supplemental Material-3
8. Comparative genome hybridization detects what type of change in the DNA?
A. Point mutations
B. Frameshift mutations
C. Alternative splicing
D. Dosage changes
E. Recombination
ANS: D
9. Which of the following techniques is used to measure the relative expression of many
transcripts simultaneously?
A. Microarray
B. Northern blot
C. Reverse transcriptase polymerase chain reaction (RT-PCR)
D. cDNA cloning
E. Comparative genome hybridization
ANS: A
10. What specifies the DNA sequence amplified in a PCR reaction?
A. The promoter and stop codon
B. The splice sites
C. The primers
D. DNA polymerase
E. The physical ends of the DNA
ANS: C
11. Which of the following techniques allows staining a particular chromosome specifically?
A. Comparative genome hybridization
B. Southern blot
C. G-banding
D. Fluorescent in situ hybridization (FISH)
E. Karyotyping
ANS: D
Copyright © . All rights reserved.
, Test Bank Online Supplemental Material-4
12. Restriction enzyme digestion would NOT be used for which of the following techniques?
A. Restriction fragment length polymorphism (RFLP)
B. Cloning a PCR fragment
C. Southern blot
D. Northern blot
E. Construction of a BAC library
ANS: D
13. A probe is required for which of the following?
A. FISH
B. Northern blot
C. Southern blot
D. Western blot
E. All of the above
ANS: E
Copyright © . All rights reserved.
