C HAPTER 11: G ENETICS OF B ACTERIA AND
A RCHAEA
Test Bank for Brock Biology of Microorganism 16th Edition by Michael T.
Madigan
11.1 Multiple Choice Questions
1. A mutant that has a nutritional requirement for growth is an example of
a(n)
A) autotroph.
B) auxotroph.
C) heterotroph.
D) organotroph.
2. Consider a mutation in which the change is from UAC to UAU. Both
codons specify the amino acid t yrosine. Which t ype of point mutation is
this?
A) silent mutation
B) nonsense mutation
C) missense mutation
D) frameshift mutation
3. If a bacterium carrying a plasmid that confers resistance to ampicillin is
placed into medium without ampicillin, it may
A) gain resistance to other antibiotics.
B) transfer resistance to other cultures in the laboratory.
C) undergo a reversion mutation.
D) lose the plasmid because there is no selection for ampicillin
resistance.
,4. Which of the following is TRUE of competence?
A) It is not required for transformation.
B) It commonl y occurs with high efficiency in nature.
C) It cannot occur naturall y in bacteria.
D) It requires special proteins such as a cell wall autol ysin.
5. A mutation that readil y reverses to restore the original parental t ype
would most likel y be due to a(n)
A) deletion.
B) insertion.
C) point mutation.
D) frameshift mutation.
6. Which process listed below al lows genetic material to be transferred from
a virus-like particle that lacks genes for its own replication?
A) conjugation of an F+ plasmid
B) gene transfer through a gene transfer agent
C) transduction by a dsDNA phage Mu
D) transformation of a linear piece of D NA
7. The mutagens 2 -aminopurine and 5-bromouracil are examples of
A) alkylating agents.
B) nucleotide base analogs.
C) chemicals reacting with DNA.
D) None of the answers are correct.
8. A deleterious mutation in recA results in
A) a decrease in specific recombinatio n.
B) a decrease in homologous recombination.
C) an increase in homologous recombination.
D) no change in either general or specific recombination.
, 9. When damaged or single-stranded DNA activates the RecA protein, the
RecA protein stimulates the cleavage of LexA . This results in
A) repression of pol ymerase V and activation of endonuclease.
B) activation of the Hfr system.
C) derepression of the SOS system.
D) increased transduction and recombination.
10.Chemical mutagens, UV radiation, and ionizing radiation all increase
mutation rates, but they have different mechanisms. Which t ype of
mutagen would be best suited for creating large deletions and
rearrangements within a genome?
A) chemical mutagens
B) UV radiation
C) ionizing radiation
D) Chemical, UV, and ionizing radiation would create large deletions
and rearrangements if used in a very high dose.
11.When DNA is transferred into a prokaryotic cell it may
A) be degraded by enz ymes.
B) replicate independent of the host chromosome.
C) recombine with the host chromosome.
D) be degraded by enz ymes, replicate independent of the host
chromosome, or recombine with the host chromosome.
12.The killing of cells by UV irradiation involves
A) absorption at 260 nm by proteins onl y.
B) absorption at 260 nm by RNA onl y.
C) formation of pyrimidine dimers.
D) formation of purine dimers.
13.Ionizing radiation does NOT include
A RCHAEA
Test Bank for Brock Biology of Microorganism 16th Edition by Michael T.
Madigan
11.1 Multiple Choice Questions
1. A mutant that has a nutritional requirement for growth is an example of
a(n)
A) autotroph.
B) auxotroph.
C) heterotroph.
D) organotroph.
2. Consider a mutation in which the change is from UAC to UAU. Both
codons specify the amino acid t yrosine. Which t ype of point mutation is
this?
A) silent mutation
B) nonsense mutation
C) missense mutation
D) frameshift mutation
3. If a bacterium carrying a plasmid that confers resistance to ampicillin is
placed into medium without ampicillin, it may
A) gain resistance to other antibiotics.
B) transfer resistance to other cultures in the laboratory.
C) undergo a reversion mutation.
D) lose the plasmid because there is no selection for ampicillin
resistance.
,4. Which of the following is TRUE of competence?
A) It is not required for transformation.
B) It commonl y occurs with high efficiency in nature.
C) It cannot occur naturall y in bacteria.
D) It requires special proteins such as a cell wall autol ysin.
5. A mutation that readil y reverses to restore the original parental t ype
would most likel y be due to a(n)
A) deletion.
B) insertion.
C) point mutation.
D) frameshift mutation.
6. Which process listed below al lows genetic material to be transferred from
a virus-like particle that lacks genes for its own replication?
A) conjugation of an F+ plasmid
B) gene transfer through a gene transfer agent
C) transduction by a dsDNA phage Mu
D) transformation of a linear piece of D NA
7. The mutagens 2 -aminopurine and 5-bromouracil are examples of
A) alkylating agents.
B) nucleotide base analogs.
C) chemicals reacting with DNA.
D) None of the answers are correct.
8. A deleterious mutation in recA results in
A) a decrease in specific recombinatio n.
B) a decrease in homologous recombination.
C) an increase in homologous recombination.
D) no change in either general or specific recombination.
, 9. When damaged or single-stranded DNA activates the RecA protein, the
RecA protein stimulates the cleavage of LexA . This results in
A) repression of pol ymerase V and activation of endonuclease.
B) activation of the Hfr system.
C) derepression of the SOS system.
D) increased transduction and recombination.
10.Chemical mutagens, UV radiation, and ionizing radiation all increase
mutation rates, but they have different mechanisms. Which t ype of
mutagen would be best suited for creating large deletions and
rearrangements within a genome?
A) chemical mutagens
B) UV radiation
C) ionizing radiation
D) Chemical, UV, and ionizing radiation would create large deletions
and rearrangements if used in a very high dose.
11.When DNA is transferred into a prokaryotic cell it may
A) be degraded by enz ymes.
B) replicate independent of the host chromosome.
C) recombine with the host chromosome.
D) be degraded by enz ymes, replicate independent of the host
chromosome, or recombine with the host chromosome.
12.The killing of cells by UV irradiation involves
A) absorption at 260 nm by proteins onl y.
B) absorption at 260 nm by RNA onl y.
C) formation of pyrimidine dimers.
D) formation of purine dimers.
13.Ionizing radiation does NOT include
