C HAPTER 12: B IOTECHNOLOGY AND
S YNTHETIC B IOLOGY
Test Bank for Brock Biology of Microorganism 16th Edition by Michael T.
Madigan
12.1 Multiple Choice Questions
1. If a foreign gene is cloned into an expression host, it is important that the
host itself
A) not produce the protein being studied.
B) produce the protein in larger amounts than the vector.
C) repress the genetic expression being studied.
D) produce signal proteins to tag the host protein.
2. What is the difference between PCR and RT -PCR?
A) Onl y PCR makes many copies of DNA rapidl y.
B) RT-PCR uses an RNA template whereas PCR uses a DNA template.
C) Onl y PCR produces cDNA.
D) PCR uses a single stranded template where as RT-PCR uses a double-
stranded template.
3. The genes encoding green fluorescent protein (GFP) and β -galactosidase
are t ypicall y used in cloning as
A) transcription regulators.
B) global control genes.
C) promoter sequences.
D) reporter genes.
,4. Which objective would be best to use a Southern blot rather than a
Northern blot?
A) Determine if a gene is present in a genome.
B) Discover gene function.
C) Identify regulatory gene -protein interactions.
D) Quantify expression profiles of a gene.
5. A pol ymerase chain reaction (P CR) copies an individual gene segment in
vitro with a(n) _______ primer(s).
A) individual RNA
B) individual DNA
C) pair of RNA
D) pair of DNA
6. Which of the following sequences is a palindrome, characteristic of many
recognition sequences for restriction endonucle ases?
A) TTGCCGA
AACGGCT
B) GGGGGGG
CCCCCCCC
C) GTAATG
CATTAC
D) GAATTC
CTTAAG
7. To estimate the total concentration of a beneficial bacterial species in
yogurt, ______ would provide the quickest results.
A) fluorescence in situ hybridization
B) qPCR
C) RT-PCR
D) a Southern blot
, 8. Which of the following is NOT a common step in molecular cloning using
plasmids?
A) Fragment DNA into small segments.
B) Hybridize DNA sequences with slightl y mismatched oligonucleotides.
C) Ligate DNA into vectors.
D) Insert the vectors into a host.
9. The principle behind nucleic acid probe design is that the probe itself
must contain
A) a key complementary part of the target gene sequence of interest.
B) all of the nucleotide sequence of the gene of interest to conclusivel y
identify the gene.
C) an antibody to specificall y bind to the gene of interest.
D) at least three separate complementary regions of the gene of interest.
10.Which of those below is NOT an important consideration when designing
a fusion protein construct?
A) Avoid hybridization of the fusion gene in the artificial construct.
B) Reading frame is the same for both the fusion gene and reporter gene.
C) Transcriptional start and stop signals are shared.
D) Translational start and stop signals are shared.
11.Cosmids are a t ype of
A) bacterial artificial chr omosomes (BACs).
B) cloning vector.
C) heat stable pol ymerase.
D) RNA/DNA hybrid.
12.Expression vectors are designed to ensure that ______ can be efficientl y
_______.
A) mRNA / transcribed
B) DNA / transcribed
S YNTHETIC B IOLOGY
Test Bank for Brock Biology of Microorganism 16th Edition by Michael T.
Madigan
12.1 Multiple Choice Questions
1. If a foreign gene is cloned into an expression host, it is important that the
host itself
A) not produce the protein being studied.
B) produce the protein in larger amounts than the vector.
C) repress the genetic expression being studied.
D) produce signal proteins to tag the host protein.
2. What is the difference between PCR and RT -PCR?
A) Onl y PCR makes many copies of DNA rapidl y.
B) RT-PCR uses an RNA template whereas PCR uses a DNA template.
C) Onl y PCR produces cDNA.
D) PCR uses a single stranded template where as RT-PCR uses a double-
stranded template.
3. The genes encoding green fluorescent protein (GFP) and β -galactosidase
are t ypicall y used in cloning as
A) transcription regulators.
B) global control genes.
C) promoter sequences.
D) reporter genes.
,4. Which objective would be best to use a Southern blot rather than a
Northern blot?
A) Determine if a gene is present in a genome.
B) Discover gene function.
C) Identify regulatory gene -protein interactions.
D) Quantify expression profiles of a gene.
5. A pol ymerase chain reaction (P CR) copies an individual gene segment in
vitro with a(n) _______ primer(s).
A) individual RNA
B) individual DNA
C) pair of RNA
D) pair of DNA
6. Which of the following sequences is a palindrome, characteristic of many
recognition sequences for restriction endonucle ases?
A) TTGCCGA
AACGGCT
B) GGGGGGG
CCCCCCCC
C) GTAATG
CATTAC
D) GAATTC
CTTAAG
7. To estimate the total concentration of a beneficial bacterial species in
yogurt, ______ would provide the quickest results.
A) fluorescence in situ hybridization
B) qPCR
C) RT-PCR
D) a Southern blot
, 8. Which of the following is NOT a common step in molecular cloning using
plasmids?
A) Fragment DNA into small segments.
B) Hybridize DNA sequences with slightl y mismatched oligonucleotides.
C) Ligate DNA into vectors.
D) Insert the vectors into a host.
9. The principle behind nucleic acid probe design is that the probe itself
must contain
A) a key complementary part of the target gene sequence of interest.
B) all of the nucleotide sequence of the gene of interest to conclusivel y
identify the gene.
C) an antibody to specificall y bind to the gene of interest.
D) at least three separate complementary regions of the gene of interest.
10.Which of those below is NOT an important consideration when designing
a fusion protein construct?
A) Avoid hybridization of the fusion gene in the artificial construct.
B) Reading frame is the same for both the fusion gene and reporter gene.
C) Transcriptional start and stop signals are shared.
D) Translational start and stop signals are shared.
11.Cosmids are a t ype of
A) bacterial artificial chr omosomes (BACs).
B) cloning vector.
C) heat stable pol ymerase.
D) RNA/DNA hybrid.
12.Expression vectors are designed to ensure that ______ can be efficientl y
_______.
A) mRNA / transcribed
B) DNA / transcribed
