ACING BIOS 255 FINAL EXAM WITH AN A+ QUESTIONS AND
ANSWERS PLAYBOOK
You are asked to inoculate a TSI (Triple Sugar Iron) slant and incubate it for 24
hours. Briefly describe how to inoculate the TSI slant
touch to colony. streak aross top of slant.
in the acronym IMViC a set of biochemical tests used for inoculating enetrics.
What does the I in it mean?
indole
in the acronym IMViC a set of biochemical tests used for inoculating enetrics.
What does the M in it mean?
Methyl Red
in the acronym IMViC a set of biochemical tests used for inoculating enetrics.
What does the V in it mean?
Vogues-Proskauer
spell the V in IMViC
Vogues-Proskauer
in the acronym IMViC a set of biochemical tests used for inoculating enetrics.
What does the C in it mean?
citrate
What is the benefit of using a multi-test kit like the ones we used in lab
Allows for quick results for many different tests to identify an organism, beneficial is
disease
treatments
What is the multi-test kit we used in lab
APle
Give 2 other general types of tests that yield the same benefit as the APIe.
Immunological Tests
Genetic Tests
Determine if the mediums listed below are selective (S), differential (D), both (B)
or neither (N) for HE and say what HE is
, HE is Hektoen Enteric Agar and it is both
Determine if the mediums listed below are selective (S), differential (D), both (B)
or neither (N) for EMB and say what EMB is
EMB is Levine Eosin Methylene Blue Agar and it is both
You inoculated a lysine decarboxylase tube and incubated it for 24 hours. After
the incubation period, you see turbidity and a yellow color. Is it positive or
negative for what? Also, what type of biochemical molecule is lysine (i.e.
carbohydrate, lipid, etc
Negative (0.5) for lysine decarboxylase (0.5 pt), Amino acid (1 pt)
You inoculated and incubated a Phenol Red (PR) Glucose tube with an unknown.
If your results end up with the medium being turbid, reddish orange, and no air
pockets in the Durham tube. What does the turbidity mean?
growth has occurred
You inoculated and incubated a Phenol Red (PR) Glucose tube with an unknown.
If your results end up with the medium being turbid, reddish orange, and no air
pockets in the Durham tube. What does the redish orange mean?
no acid production and no gluclose
You inoculated and incubated a Phenol Red (PR) Glucose tube with an unknown.
If your results end up with the medium being turbid, reddish orange, and no air
pockets in the Durham tube. What does the no air pockets mean?
no gas production
You stabbed the motility test medium using the inoculating needle with your
unknown. What would indicate that your unknown was motile?
Cloudiness in ALL directions from the stab line
If your unknown was motile, give the 3 types of motility that it may have.
Swimming (or Swarming), Gliding, Twitching
Requires 𝑂2 for growth
Obligate Aerobe
Facultative Anaerobe_
Grows best in the presence of 𝑂2 but not essential
Microaerophile
ANSWERS PLAYBOOK
You are asked to inoculate a TSI (Triple Sugar Iron) slant and incubate it for 24
hours. Briefly describe how to inoculate the TSI slant
touch to colony. streak aross top of slant.
in the acronym IMViC a set of biochemical tests used for inoculating enetrics.
What does the I in it mean?
indole
in the acronym IMViC a set of biochemical tests used for inoculating enetrics.
What does the M in it mean?
Methyl Red
in the acronym IMViC a set of biochemical tests used for inoculating enetrics.
What does the V in it mean?
Vogues-Proskauer
spell the V in IMViC
Vogues-Proskauer
in the acronym IMViC a set of biochemical tests used for inoculating enetrics.
What does the C in it mean?
citrate
What is the benefit of using a multi-test kit like the ones we used in lab
Allows for quick results for many different tests to identify an organism, beneficial is
disease
treatments
What is the multi-test kit we used in lab
APle
Give 2 other general types of tests that yield the same benefit as the APIe.
Immunological Tests
Genetic Tests
Determine if the mediums listed below are selective (S), differential (D), both (B)
or neither (N) for HE and say what HE is
, HE is Hektoen Enteric Agar and it is both
Determine if the mediums listed below are selective (S), differential (D), both (B)
or neither (N) for EMB and say what EMB is
EMB is Levine Eosin Methylene Blue Agar and it is both
You inoculated a lysine decarboxylase tube and incubated it for 24 hours. After
the incubation period, you see turbidity and a yellow color. Is it positive or
negative for what? Also, what type of biochemical molecule is lysine (i.e.
carbohydrate, lipid, etc
Negative (0.5) for lysine decarboxylase (0.5 pt), Amino acid (1 pt)
You inoculated and incubated a Phenol Red (PR) Glucose tube with an unknown.
If your results end up with the medium being turbid, reddish orange, and no air
pockets in the Durham tube. What does the turbidity mean?
growth has occurred
You inoculated and incubated a Phenol Red (PR) Glucose tube with an unknown.
If your results end up with the medium being turbid, reddish orange, and no air
pockets in the Durham tube. What does the redish orange mean?
no acid production and no gluclose
You inoculated and incubated a Phenol Red (PR) Glucose tube with an unknown.
If your results end up with the medium being turbid, reddish orange, and no air
pockets in the Durham tube. What does the no air pockets mean?
no gas production
You stabbed the motility test medium using the inoculating needle with your
unknown. What would indicate that your unknown was motile?
Cloudiness in ALL directions from the stab line
If your unknown was motile, give the 3 types of motility that it may have.
Swimming (or Swarming), Gliding, Twitching
Requires 𝑂2 for growth
Obligate Aerobe
Facultative Anaerobe_
Grows best in the presence of 𝑂2 but not essential
Microaerophile
