m m m m m m m
d Edition By John W Foster, Zarrintaj Aliabadi, Joan L S
m m m m m m m m m m
lonczewski
Whatmismthemconceptmofmspontaneousmgeneration?m-
mANSWERmmmlivingmorganismsmcouldmdevelopmfrommnonlivingmormdecomposingmmatter
Whomchallengedmthemconceptmofmspontaneousmgenerationmbymshowingmthatmmaggotsm
onmdecayingmmeatmcamemfrommflies,mnotmfrommthemmeatmitself?m-
mANSWERmmmFrancescomRedi
Whomshowedmthatmmuttonmbrothmboiledminmflasksmandmthenmsealedmcouldmstillmdevelopm
microorganisms,mwhichmsupportedmthemtheorymofmspontaneousmgeneration?m-
mANSWERmmmJohnmNeedham
Whomshowedmthatmflasksmsealedmandmthenmboiledmhadmnomgrowthmofmmicroorganisms?
m-mANSWERmmmLazzaro mSpallanzani
Whomproposedmthatmairmcarriedmgerms?m-mANSWERmmmSpallanzani
Whomdisprovedmthemtheorymofmspontaneousmgeneration?m-mANSWERmmmLouismPasteur
Whomdemonstratedmthatmdustmcarriedmmicrobesmandmprovedmevidencemformthemexisten
cemofmheat-mresistantmformsmofmbacteria?m-mANSWERmmmJohnmTyndall
Whomshowedmthatmamsilkwormmdiseasemwasmcausedmbymamfungus?m-
mANSWERmmmAgostinomBassi
WhomdemonstratedmthatmthemGreatmPotatomBlightmwasmcausedmbymamwatermmold?m-
mANSWERmmmM.mJ.mBerkely
Whomshowedmthatmthempebrinemdiseasemofmsilkwormsmwasmcausedmbymamprotozoanmpar
asite?m-mANSWERmmmLouismPasteur
Whomdevelopedmamsystemmofmsurgerymdesignedmtompreventmmicroorganismsmfromment
eringmwounds?m-mANSWERmmmJohnmLister
Whomtransformedmthempracticemofmsurgery?m-mANSWERmmmJohnmLister
WhomestablishedmthemrelationshipmbetweenmBacillusmanthracismandmanthrax?m-
mANSWERmmmRobertmKoch
WhatmaremthemKochmPostulates?m-
mANSWERmmm1.Themmicroorganismsmmustmbempresentminmeverymcasemofmthemdiseasemb
utmabsentmfrommhealthymindividualsm
2.Themsuspectedmmicroorganismsmmustmbemisolatedmandmgrownminmpuremculturem
,3.mThemsamemdiseasemmustmresultmwhenmthemisolatedmmicroorganismmisminoculatedmint
omamhealthymhostm
4.mThemsamemmicroorganismmmustmbemisolatedmagainmfrommthemdiseasedmhost
LimitationsmofmKoch'smPostulatesm-mANSWERmmm-
Somemorganismsmcannotmbemgrownminmpuremculture
-Usingmhumansminmcompletingmthempostulatesmismunethical
-Molecularmandmgeneticmevidencemmaymreplace
WhatmvaccinationmdidmEdwardmJennermmake?m-mANSWERmmmsmallpoxmvaccination
WhatmvaccinationsmdidmPasteurmproduce?m-
mANSWERmmmchickenmcholera,mrabiesmandmanthrax
Whominducedmformationmofmdippedmtoxin-antitoxinsmrabbitsm-
mANSWERmmmEmilmvonmBehringmandmShibasaburomKitasato
Whomshowedmthatmthemsilkwormmdiseasemwasmcausedmbymfungus?m-mANSWERmmmBassi
WhomdemonstratedmthatmthemGreatmPotatomBlightmofmIrelandmwasmcausedmbymamwaterm
mold?m-mANSWERmmmM.J.mBerkeley
Whomshowedmthatmthemperbrinemdiseasemofmsilkwormsmwasmcausedmbymamprotozoanmpa
rasite?m-mANSWERmmmLouismPasteur
Amsimplemmorphologymandmlackmamtruemmembrane-delimitedmnucleusm-
mANSWERmmmprokaryotes
Whatmismammorphologicallymcomplexmandmhasmammembrane-enclosedmnucleus?m-
mANSWERmmmEucaryotes
Whatmletmtomthemdiscoverymofmvirusesmasmdiseasemcausingmagents?m-
mANSWERmmmbacterialmfiltermthatmremovedmbacteriamandmlargermmicrobesm(CharlesmCha
mberland)
Themfivemkingdommclassificationmschememism-
mANSWERmmmthemMoneramormProcaryotae,mProtista,mFungi,mAnimalia,mandmPlantae
Whatmaremribozymes?m-
mANSWERmmmRNAmmoleculesmthatmformmpeptidembondsmandmperformmcellularmworkmand
mreplication
Themthreemdomainmschememism-mANSWERmmmBacteria,mArchaea,mandmEucarya
Themthreemdomainmschememismbasedmon?m-
mANSWERmmmamcomparaisonmofmribosomalmRNA
Themearliestmcellsmmaymhavembeenm-mANSWERmmmRNAmsurroundedmbymliposomes
Whatmaremthemthreembacterialmmorphology?m-mANSWERmmm-bacillusm(littlemrod)
,-coccusm(grainmormberry)
-spirillumm(coiledmormhelical)
Whatmaremthemtypesmofmlightmmicroscopes?m-mANSWERmmm-bright-fieldmmicroscope
-dark-fieldmmicroscope
-phase-contrastmmicroscope
-fluorescencemmicroscopes
Whatmismammeasuremofmhowmgreatlymamsubstancemslowsmthemvelocitymofmlight?m-
mANSWERmmmRefractivemindex
Themdirectionmandmmagnitudemofmbendingmlightminmammicroscopemismdeterminedmbymthem
_____m-mANSWERmmmrefractivemindexesmofmthemtwommediamformingmtheminterface
Whichmmicroscopemproducesmamdarkmimagemagainstmambrightermbackground?m-
mANSWERmmmthembright-fieldmmicroscope
Whatmismthemproductmofmthemmagnificationsmofmthemocularmlensmandmthemobjectivemlens?
m-mANSWERmmmthemtotalmmagnification
Whatmismthemtotalmmagnificationmofmthembrightmfield,mdarkmfield,mandmphase-
contrastmmicroscopes?m-mANSWERmmm2000x
Whatmismthemtotalmresolutionmofmthembrightmfield,mdarkmfield,mandmphasemcontrastmmicros
copes?m-mANSWERmmm0.2um
Whatmismthempurposemofmthembrightmfieldmmicroscope?m-
mANSWERmmmformlivemandmpreservedmstainedmspecimens
Whatmismthempurposemofmthemdarkmfieldmmicroscope?m-
mANSWERmmmtomobservemliving,munstainedmpreparations
Whichmmicroscopesmaremgoodmformobservingmlivingmcells?m-mANSWERmmm-
ThemDifferentialmInterferencemContrastmMicroscope
-Phase-contrastmmicroscope
Whichmmicroscopemenhancesmthemcontrastmbetweenmintracellularmstructuresmhavingmsli
ghtmdifferencesminmthemrefractivemindex?m-mANSWERmmmphase-contrastmmicroscope
Whichmmicroscopemshowsmambrightmimagemofmthemobjectmresultingmfrommthemfluorescen
tmlightmemittedmbymthemspecimen?m-mANSWERmmmthemfluorescencemmicroscope
Themfluorescencemmicroscopemexposesmthemspecimenmtomwhatmtypesmofmlight?m-
mANSWERmmmuv,mviolet,mormblue
Themdistancembetweenmthemcentermofmthemlensmandmfocalmpoint?m-
mANSWERmmmfocalmlength
Themshortermthemfocalmpoint,mthem______mthemmagnificationm-mANSWERmmmgreater
, Themshortermthemwavelength,mthem______mthemresolutionm-mANSWERmmmgreater
Themdistancembetweenmthemfrontmsurfacemofmlensmandmsurfacemofmcovermglassmonmspeci
menm-mANSWERmmmWorkingmdistance
Asmthemworkingmdistancemdecrease,mthemfieldmofmviewm_____m-mANSWERmmmdecrease
Asmthemfieldmofmviewmdecrease,mthemlightmgetsm____m-mANSWERmmmdimmer
Whatmismthempurposemofmaddingmthemimmersionmoil?m-
mANSWERmmmtomobtainmmoremlightmbymprovidingmthemsamemrefractionmenergymasmglass
Asmthemmagnificationmincreases,mwhatmhappensmtomthemfocalmlength?m-
mANSWERmmmitmdecrease
Whatmismthemchargemofmambacteriamcellmsurface?m-mANSWERmmmnegativemcharge
Whatmismthemchargemofmbasicmdyes?m-mANSWERmmmpositivemcharge
Whatmismthemchargemofmacidmdyes?m-mANSWERmmmnegativemcharge
Amthin,muniformmfilmmofmbacterialmgrowthmonmamglassmslideminmordermtomproceedmwithmfurt
hermstainingmformmicroscopicmexaminationm-mANSWERmmmsmear
Themprocessmbymwhichmorganismmismkilledmandmfirmlymattachedmtommicroscopemslidem-
mANSWERmmmfixation
Heatmfixationmvs.mChemicalmfixingm(notmthemprocedure)m-mANSWERmmm-
heatmfixationmpreservesmoverallmshapembutmnotminternalmstructures
-
chemicalmfixingmprotectsmfinemcellularmstructuremandmshapemofmlarger,mmoremdelicatemor
ganisms
Whatminformationmdoesmdifferentialmstainingmprovide?m-
mANSWERmmmdividesmmicroorganismsmintomgroupsmbasedmonmtheirmstainingmproperties
Whatmismthemmostmwidelymusedmdifferentialmstainingmprocedure?m-
mANSWERmmmgrammstaining
Negativemstainingm-
mANSWERmmmthembacteriamismnotmstained;mthembackgroundmismstained
Whatmismshadowingminmelectronmmicroscopy?m-
mANSWERmmmcoatingmspecimenmwithmamthinmfilmmofmheavymmetal
WhatmismthemtotalmmagnificationmofmthemTransmissionmElectronmMicroscopemandmthemSc
anningmElectronmMicroscope?m-mANSWERmmm1,000,000x
WhatmismthemtotalmmagnificationmofmthemScanningmElectronmMicroscope?m-
mANSWERmmm1,000,000x