C HAPTER 19: T AKING THE M EASURE OF
M ICROBIAL S YSTEMS
Test Bank for Brock Biology of Mic roorganism 16th Edition by Michael T.
Madigan
19.1 Multiple Choice Questions
1. The science of microbial ecology deals with
A) how microbial communities interact with each other and their
environment.
B) microbial activit y and biodiversit y.
C) microbial gene regulation mechanisms in response to environmental
change.
D) how the activit y and biodiversit y of microbial communities affect
microbial interactions with each other and the environment.
2. The methodology of microbial ecology includes
A) enrichment and isolation of specific microbes.
B) cell-staining methods.
C) gene, transcript, and protein characterization.
D) enrichment and isolation of specific microbes, cell -staining, and
characterization of genes, transcripts, and proteins.
3. In an agar dilution tube, microbial c olonies grow
A) at the bottom of the tube.
B) on the sides of the tube, although some may be at the surface.
C) embedded in the agar, rather than growing on the surface.
D) on the surface, but some creep down the sides away from the surface.
,4. Which of the followi ng statements is FALSE concerning enrichment bias?
A) Dilution of the inoculum may help to avoid enrichment bias in some
cases.
B) Enrichment bias is impossible to overcome in some oligotrophic
environments.
C) Enrichment bias resulted in the underestimation of prokaryotic
diversit y.
D) Enrichment bias favors organisms that grow quickl y.
5. Which fluorescent molecule enables visualization of living
microorganisms?
A) acridine orange
B) DAP I
C) GFP
D) SYBR® Green
6. Laser tweezers are most directl y used for
A) enriching a culture.
B) isolating individual cells.
C) increasing magnification.
D) enhancing the growth of fastidious organisms.
7. Which molecular method allows for the detection of a specific organism
within a diverse microbial communit y?
A) fluorescent DNA staining using DAP I
B) viabilit y staining
C) enrichment techniques
D) fluorescent in situ hybridization (FISH)
8. Metatranscriptomics and metaproteomics are favored over metagenomics
when ________ is of more interest than ______.
A) gene presence / phyl ogeny
, B) phylogeny / gene presence
C) function or gene expression / gene presence
D) gene presence / function or gene expression
Chapter Section: 19.8
9. What are activit y measurements?
A) Activit y measurements are any measurements taken in vitro.
B) Activit y measurements are any measurements take n in situ.
C) Activit y measurements are collective estimates of the physiological
reactions in an entire microbial community.
D) Activit y measurements are an example of metagenomics, which can
be used when genetic anal ysis is difficult.
10.Which approach would help to identify biologicall y produced sulfur in a
sediment sample?
A) enrichment
B) isotopic fractionation
C) microautoradiography
D) microelectrodes
11.When laser tweezers are used, the laser beam can trap
A) an isolated single -copy genome in a capillary tube.
B) a single microbial cell in a capillary tube.
C) a single microbial colony on a plate.
D) multiple cells of the same t ype on a plate.
12.Which of the following techniques can link prokaryotic phylogeny and
metabolic function?
A) DAP I staining
B) stable isotope probing onl y
M ICROBIAL S YSTEMS
Test Bank for Brock Biology of Mic roorganism 16th Edition by Michael T.
Madigan
19.1 Multiple Choice Questions
1. The science of microbial ecology deals with
A) how microbial communities interact with each other and their
environment.
B) microbial activit y and biodiversit y.
C) microbial gene regulation mechanisms in response to environmental
change.
D) how the activit y and biodiversit y of microbial communities affect
microbial interactions with each other and the environment.
2. The methodology of microbial ecology includes
A) enrichment and isolation of specific microbes.
B) cell-staining methods.
C) gene, transcript, and protein characterization.
D) enrichment and isolation of specific microbes, cell -staining, and
characterization of genes, transcripts, and proteins.
3. In an agar dilution tube, microbial c olonies grow
A) at the bottom of the tube.
B) on the sides of the tube, although some may be at the surface.
C) embedded in the agar, rather than growing on the surface.
D) on the surface, but some creep down the sides away from the surface.
,4. Which of the followi ng statements is FALSE concerning enrichment bias?
A) Dilution of the inoculum may help to avoid enrichment bias in some
cases.
B) Enrichment bias is impossible to overcome in some oligotrophic
environments.
C) Enrichment bias resulted in the underestimation of prokaryotic
diversit y.
D) Enrichment bias favors organisms that grow quickl y.
5. Which fluorescent molecule enables visualization of living
microorganisms?
A) acridine orange
B) DAP I
C) GFP
D) SYBR® Green
6. Laser tweezers are most directl y used for
A) enriching a culture.
B) isolating individual cells.
C) increasing magnification.
D) enhancing the growth of fastidious organisms.
7. Which molecular method allows for the detection of a specific organism
within a diverse microbial communit y?
A) fluorescent DNA staining using DAP I
B) viabilit y staining
C) enrichment techniques
D) fluorescent in situ hybridization (FISH)
8. Metatranscriptomics and metaproteomics are favored over metagenomics
when ________ is of more interest than ______.
A) gene presence / phyl ogeny
, B) phylogeny / gene presence
C) function or gene expression / gene presence
D) gene presence / function or gene expression
Chapter Section: 19.8
9. What are activit y measurements?
A) Activit y measurements are any measurements taken in vitro.
B) Activit y measurements are any measurements take n in situ.
C) Activit y measurements are collective estimates of the physiological
reactions in an entire microbial community.
D) Activit y measurements are an example of metagenomics, which can
be used when genetic anal ysis is difficult.
10.Which approach would help to identify biologicall y produced sulfur in a
sediment sample?
A) enrichment
B) isotopic fractionation
C) microautoradiography
D) microelectrodes
11.When laser tweezers are used, the laser beam can trap
A) an isolated single -copy genome in a capillary tube.
B) a single microbial cell in a capillary tube.
C) a single microbial colony on a plate.
D) multiple cells of the same t ype on a plate.
12.Which of the following techniques can link prokaryotic phylogeny and
metabolic function?
A) DAP I staining
B) stable isotope probing onl y
