BLD 435 EXAM 2 QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED
How to tell A2 from Asubs? What reagent do you use that agglutinates only A1
cells and not others?
A1 lectin or absorbed anti-A1
A1 lectin.
Dolichos biflorus. from plant seeds grinded with saline, agglutinate red cells, NOT an
antibody but acts like one
Absorbed anti-A1; B people make anti-A1, but it is really...? What do you remove?
anti-A1 and anti-Agroup. Remove the antigroup and use the anti-A1 (part of it
agglutinates A1 cells, part of it aggl all of it, have to absorb out everything but Anti-A1,
use A2 cells!!!)
How can you check to see if they make Anti-A1?
by reverse typing with A1 and A2 cells (normal reverse typing-use A1 cells)
Does A1, 2, or 3 always give mixed field agglutination?
A3
Mixed field agglutination reasons?
bone marrow transplant (induced chimerism), true chimera, recent transfusion, A3
A or B subgroups. May see ____ reactions forward. For every A2, is a ____ blood
group.
weak, B2
, High level of soluble antigens in sera can do what to reagents?
neutralize, to prevent WASH
Are A1 and B Rh positive or neg?
Neg
All sites for antibody interaction can be blocked by mass...?
C1 binding
What do you do when giving blood to a patient with a recent plasma transfusion?
give group A AB plasma, diluted Anti-B out, reverse type may now be 2+ or weaker. add
3-5 drops, incubate longer at lower temp, IgM, reacts at cold temp (put it in fridge), use
enzymes, check saliva (80%)
Acquired A vs. Acquired B
Acquired A: Tn activated. if right circumstance, can get hidden/cryptic antigens
exposed, made antis against them because were hidden, will have anti-T and recognize
it.
Acquired B: have to be Group A, bacteria makes acetylase, breaks down acetyl groups,
leaves galactose (B), treat with access antibiotics until its back to 4+ and 0. (Anti-A gets
weaker graded, Anti-B gets stronger graded, 1+ -> 2+ -> 3+)
Resolving problems with back typing. To see reactions better?
add more serum, decrease temp, incubate longer
How to resolve rouleaux. Problem with REVERSE because cells not washed.
saline replacement technique. take supernatant off, add back equal volume of saline (2
drops)
Clinically significant antibodies.
COMPLETE SOLUTIONS VERIFIED
How to tell A2 from Asubs? What reagent do you use that agglutinates only A1
cells and not others?
A1 lectin or absorbed anti-A1
A1 lectin.
Dolichos biflorus. from plant seeds grinded with saline, agglutinate red cells, NOT an
antibody but acts like one
Absorbed anti-A1; B people make anti-A1, but it is really...? What do you remove?
anti-A1 and anti-Agroup. Remove the antigroup and use the anti-A1 (part of it
agglutinates A1 cells, part of it aggl all of it, have to absorb out everything but Anti-A1,
use A2 cells!!!)
How can you check to see if they make Anti-A1?
by reverse typing with A1 and A2 cells (normal reverse typing-use A1 cells)
Does A1, 2, or 3 always give mixed field agglutination?
A3
Mixed field agglutination reasons?
bone marrow transplant (induced chimerism), true chimera, recent transfusion, A3
A or B subgroups. May see ____ reactions forward. For every A2, is a ____ blood
group.
weak, B2
, High level of soluble antigens in sera can do what to reagents?
neutralize, to prevent WASH
Are A1 and B Rh positive or neg?
Neg
All sites for antibody interaction can be blocked by mass...?
C1 binding
What do you do when giving blood to a patient with a recent plasma transfusion?
give group A AB plasma, diluted Anti-B out, reverse type may now be 2+ or weaker. add
3-5 drops, incubate longer at lower temp, IgM, reacts at cold temp (put it in fridge), use
enzymes, check saliva (80%)
Acquired A vs. Acquired B
Acquired A: Tn activated. if right circumstance, can get hidden/cryptic antigens
exposed, made antis against them because were hidden, will have anti-T and recognize
it.
Acquired B: have to be Group A, bacteria makes acetylase, breaks down acetyl groups,
leaves galactose (B), treat with access antibiotics until its back to 4+ and 0. (Anti-A gets
weaker graded, Anti-B gets stronger graded, 1+ -> 2+ -> 3+)
Resolving problems with back typing. To see reactions better?
add more serum, decrease temp, incubate longer
How to resolve rouleaux. Problem with REVERSE because cells not washed.
saline replacement technique. take supernatant off, add back equal volume of saline (2
drops)
Clinically significant antibodies.
