COMPLETE SOLUTIONS VERIFIED GRADED A++ LATEST
UPDATE
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The steps of working through an unexpected antibody are:
1. detection
2. identification
3. confirmation
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The panel cells used to make a presumptive identification of an unexpected antibody
come from different donors who are group O.
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Panel cells used to make a presumptive ID of an unexpected antibody are antigen typed
for the more common antigens we encounter antibodies against and the "+"'s and "0"'s
we see on the antigen profile sheet reflect their antigen make up.
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, We review how our patient reacts with the cells in the panel and try to match our pattern
of reactivity with the reactivity pattern of the cells when they were typed at the
manufacturer, in other words, we look to see if our patient serum reactions are negative
where the antigen is lacking and positive where the antigen is present.
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The panel cells that our patient does not react with can be used to "cross out" antigens
that are present on that cell and therefore thought not to be the specificity of the
antibody present.
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The underlying assumption of "crossing out" is that since we know we have given the
antibody the opportunity to react with the antigens present on that cell, we make the
assumption if the cell is negative with our patient's serum, that the specificity of the
antibody in our patient is not on that cell.
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Once a presumptive identification is made, we must confirm by:
1. antigen typing the patient to confirm they are antigen negative and therefore they
could make that antibody.
2. run a select cell panel that is made up of 3 cells positive for the presumed antigen
and 3 cells negative, the patient pattern must match the antigen profile of the six cells