MDLB 1521 MODULE B - MICROBIOLOGICAL SPECIMENS QUESTIONS WITH COMPLETE

MDLB 1521 MODULE B -
MICROBIOLOGICAL SPECIMENS
QUESTIONS WITH COMPLETE
SOLUTIONS.


What are the four main groups of microbiological specimens? - ANSWER:Bacteria,
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fungi, parasites, and viruses
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Blood fluid specimens include - ANSWER:Blood, urine, cerebrospinal fluid (CSF),
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respiratory specimens (sputum), and other fluids (synovial, pleural, etc.)
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Safety procedures in the collection of specimens - ANSWER:Follow standard
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precautions, use appropriate PPE, do not contaminate the external surface of the
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collection container or the requisition, minimize direct handling of specimens in transit
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from collection point to lab, and use plastic, sealable bags with a separate pouch for
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paperwork
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Whenever possible, specimens for culture are collected _____________ administration
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of antibiotics - ANSWER:Before
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Specimens should be collected with as little - ANSWER:Contamination from normal
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flora as possible to ensure that the sample will be representative of the infected site.
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Always collect an _______________ amount of specimen for testing -
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ANSWER:Adequate
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It is crucial to ___________ specimens correctly with the _____________, and
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_____________. - ANSWER:Specific source and specific site. So that the appropriate
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culture media can be selected during processing
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Using appropriate collection devices, specimen containers, and transport media
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ensures - ANSWER:Preservation of viability of microorganisms, ensuring their optimal
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recovery.
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Specimens should be collected in - ANSWER:Sterile, sturdy, screw-cap, leak-proof
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containers with kids that do not create an aerosol when opened
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, Culture container labels should include - ANSWER:Patient's full name, hospital number
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or equivalent, specimen source, & time and date of collection
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commensal organisms - ANSWER:bacteria normally found at various non-sterile body
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sites but do no harm
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Specimens are collected into or onto what? - ANSWER:Sterile containers or sterile
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culture swabs
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Culture swabs are used for - ANSWER:Culture and sensitivity testing of various body
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parts
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Culture swabs have a _______________ or a __________________ in the bottom of
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the container to keep the bacteria alive during transport. - ANSWER:Transport gel or
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a moistened sponge
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Physicians or nurses rather than MLAs will collect cultures from - ANSWER:Swabs
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from wounds, body fluids, respiratory tract, and genital specimens
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Because urine is a good growth medium in which organisms will multiply rapidly, urine
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must be - ANSWER:Either cultures within 2 hours of collection or refrigerated (4
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degrees C) for up to 24 hours until it can be cultured.
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An alternative to refrigeration for urines is a tube with a preservative called -
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ANSWER:Boric acid-glycerol-sodium formate - maintains a stable bacterial population
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for up to 48 hours.
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CSF is obtained from - ANSWER:A spinal tan or lumbar puncture
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CSF is collected in - ANSWER:3-4 separate tubes. The first goes to chemistry (for
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protein and glucose), the second to microbiology (for culture), the third to hematology
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(for cell counts & differentials), and the fourth is sometimes kept aside for further
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testing just in case.
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CSF are always considered - ANSWER:STAT and processes immediately - never
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refrigerated before culturing
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Body fluids are especially critical specimens because - ANSWER:They require
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invasive collection procedures and generally indicate a systemic infection throughout
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the body
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Vaginal swabs - ANSWER:Examined for bacterial vaginosis, yeast infections, &
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Trichomonas (a parasite)
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Cervix and urethral swabs - ANSWER:Tested for gonorrhea and chlamydia via NAAT
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method
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