MLS Chemistry Exam with correct
solutions
Which of the following lamps provides a continuous spectrum of radiant
n n n n n n n n n n n
energy in the visible, near IR and near UV regions of the spectrum? - correct
n n n n n n n n n n n n n n
nanswer ✔Tungsten filament
n n
Which of the following isolates light within a narrow region of the
n n n n n n n n n n n n
spectrum? - correct answer ✔Monochromator
n n n n
Which of the following is not descriptive of a photomultiplier tube? - correct
n n n n n n n n n n n n
answer ✔Cannot be used with a chopper
n n n n n n n
Which of the following is false about a photomultiplier tube? - correct
n n n n n n n n n n n n
answer ✔Is composed of an iron plate and a layer of selenium
n n n n n n n n n n n
Which type of photodetector employs a linear arrangement that allows it to
n n n n n n n n n n n n
respond to a specific wavelength resulting in complete UV visible spectrum
n n n n n n n n n n n
analysis? - correct answer ✔Photodiode array
n n n n n
When performing spectrophotometer quality assurance checks, what is the
n n n n n n n n n
holmium oxide glass filter used to assess? - correct answer ✔Wavelength
n n n n n n n n n n n
accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank? -
n n n n n n n n n n n n
correct answer ✔Correct for color contribution of the reagents
n n n n n n n n
,In regard to bichromatic analysis, which of the following is false? - correct
n n n n n n n n n n n n n
answer ✔Absorbance is measured at the spectral absorbance peak for a
n n n n n n n n n n n
blank and the sample using the same wavelength
n n n n n n n
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at
n n n n n n n n n n n n n n
540 nm, the wavelengths that are permitted to impinge on the sample will
n n n n n n n n n n n n n
be within what wavelength range? - correct answer ✔535 to 545 nm
n n n n n n n n n n n
Which of the following formulas is an expression of the Beer Lambert law
n n n n n n n n n n n n n
that is routinely applied to spectrophotometric analysis? - correct answer
n n n n n n n n n n
✔Au X Cs / As = Cun n n n n n
In spectrophotometry, which of the following is a mathematical expression
n n n n n n n n n n
of the relationship between absorbance and transmittance? - correct answer
n n n n n n n n n n
✔A = 2 - log % T
n n n n n n
Which of the following is not a problem inherent in turbidimetry? - correct
n n n n n n n n n n n n n
answer ✔Need to maintain a constant and specific temperature
n n n n n n n n
Which of the following may be associated with reflectance
n n n n n n n n n
spectrophotometry as it relates to the dry reagent slide technique? - correct n n n n n n n n n n n n
answer ✔Unabsorbed, reflected light detected by photodetector
n n n n n n
Fluorometers are designed so that the path of the exciting light is at a right
n n n n n n n n n n n n n n n
angle to the path of the emitted light. What is the purpose of this design? -
n n n n n n n n n n n n n n n n
correct answer ✔Prevent excitation light from reaching the detector
n n n n n n n n
,Which of the following represents a primary advantage of performing
n n n n n n n n n n
fluorometric over absorption spectroscopic methods of analysis? - correct
n n n n n n n n n
answer ✔Increased specificity and increased sensitivity
n n n n n
Which of the following may be associated with fluorescence polarization? -
n n n n n n n n n n n
correct answer ✔Plane polarized light is used for sample excitation
n n n n n n n n n
Which of the following may be associated with bioluminescence? - correct
n n n n n n n n n n n
answer ✔Light emission produced due to enzymatic oxidation of a substrate
n n n n n n n n n n
Nephelometry is based on the measurement of light that is: - correct answer
n n n n n n n n n n n n
✔Scattered by particles in suspension
n n n n n
Which of the following instruments is used in the clinical lab or in reference
n n n n n n n n n n n n n n
labs to detect beta and gamma emissions? - correct answer ✔Scintillation
n n n n n n n n n n n
counter
Which of the following best describes chemiluminescence? - correct answer
n n n n n n n n n n
✔Chemical energy excites electrons that emit light upon return to ground
n n n n n n n n n n n
state
In assaying an analyte with a single beam atomic absorption
n n n n n n n n n n
spectrophotometer, what is the instrument actually measuring? - correct n n n n n n n n n
answer ✔Intensity of the beam from the hollow cathode lamp after it has
n n n n n n n n n n n n n
passed through the analyte containing flame
n n n n n
What is the function of the flame in atomic absorption spectroscopy? -
n n n n n n n n n n n n
correct answer ✔Bring the metal analyte to its ground state
n n n n n n n n n
, Most atomic absorption spectrophotometers incorporate a beam chopper
n n n n n n n n
and a tuned amplifier. The purpose of these components is to avoid errors
n n n n n n n n n n n n n
that would be caused by: - correct answer ✔Measurement of light emitted
n n n n n n n n n n n n
by the analyte
n n
In potentiometry, which of the following is considered the standard
n n n n n n n n n n
electrode? - correct answer ✔Hydrogen electrode
n n n n n
In an electrolytic cell, which of the following is the half cell where reduction
n n n n n n n n n n n n n n
takes place? - correct answer ✔Cathode
n n n n n
Mercury covered by a layer of mercurous chloride in contact with saturated
n n n n n n n n n n n n
potassium chloride solution is a description of which of the following types
n n n n n n n n n n n n
of electrodes? - correct answer ✔Calomel
n n n n n
When a pH sensitive glass electrode is not actively in use, in what type of
n n n n n n n n n n n n n n n
solution should it be kept? - correct answer ✔The medium recommended by
n n n n n n n n n n n
nthe manufacturer
n
When measuring K positive with an ion selective electrode by means of a
n n n n n n n n n n n n n
liquid ion exchange membrane, what antibiotic will be incorporated into the
n n n n n n n n n n n
membrane? - correct answer ✔Valinomycin n n n n
Which of the following is false about ion selective electrode analysis of
n n n n n n n n n n n n
sodium? - correct answer ✔Membrane coated with valinomycin
n n n n n n n
solutions
Which of the following lamps provides a continuous spectrum of radiant
n n n n n n n n n n n
energy in the visible, near IR and near UV regions of the spectrum? - correct
n n n n n n n n n n n n n n
nanswer ✔Tungsten filament
n n
Which of the following isolates light within a narrow region of the
n n n n n n n n n n n n
spectrum? - correct answer ✔Monochromator
n n n n
Which of the following is not descriptive of a photomultiplier tube? - correct
n n n n n n n n n n n n
answer ✔Cannot be used with a chopper
n n n n n n n
Which of the following is false about a photomultiplier tube? - correct
n n n n n n n n n n n n
answer ✔Is composed of an iron plate and a layer of selenium
n n n n n n n n n n n
Which type of photodetector employs a linear arrangement that allows it to
n n n n n n n n n n n n
respond to a specific wavelength resulting in complete UV visible spectrum
n n n n n n n n n n n
analysis? - correct answer ✔Photodiode array
n n n n n
When performing spectrophotometer quality assurance checks, what is the
n n n n n n n n n
holmium oxide glass filter used to assess? - correct answer ✔Wavelength
n n n n n n n n n n n
accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank? -
n n n n n n n n n n n n
correct answer ✔Correct for color contribution of the reagents
n n n n n n n n
,In regard to bichromatic analysis, which of the following is false? - correct
n n n n n n n n n n n n n
answer ✔Absorbance is measured at the spectral absorbance peak for a
n n n n n n n n n n n
blank and the sample using the same wavelength
n n n n n n n
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at
n n n n n n n n n n n n n n
540 nm, the wavelengths that are permitted to impinge on the sample will
n n n n n n n n n n n n n
be within what wavelength range? - correct answer ✔535 to 545 nm
n n n n n n n n n n n
Which of the following formulas is an expression of the Beer Lambert law
n n n n n n n n n n n n n
that is routinely applied to spectrophotometric analysis? - correct answer
n n n n n n n n n n
✔Au X Cs / As = Cun n n n n n
In spectrophotometry, which of the following is a mathematical expression
n n n n n n n n n n
of the relationship between absorbance and transmittance? - correct answer
n n n n n n n n n n
✔A = 2 - log % T
n n n n n n
Which of the following is not a problem inherent in turbidimetry? - correct
n n n n n n n n n n n n n
answer ✔Need to maintain a constant and specific temperature
n n n n n n n n
Which of the following may be associated with reflectance
n n n n n n n n n
spectrophotometry as it relates to the dry reagent slide technique? - correct n n n n n n n n n n n n
answer ✔Unabsorbed, reflected light detected by photodetector
n n n n n n
Fluorometers are designed so that the path of the exciting light is at a right
n n n n n n n n n n n n n n n
angle to the path of the emitted light. What is the purpose of this design? -
n n n n n n n n n n n n n n n n
correct answer ✔Prevent excitation light from reaching the detector
n n n n n n n n
,Which of the following represents a primary advantage of performing
n n n n n n n n n n
fluorometric over absorption spectroscopic methods of analysis? - correct
n n n n n n n n n
answer ✔Increased specificity and increased sensitivity
n n n n n
Which of the following may be associated with fluorescence polarization? -
n n n n n n n n n n n
correct answer ✔Plane polarized light is used for sample excitation
n n n n n n n n n
Which of the following may be associated with bioluminescence? - correct
n n n n n n n n n n n
answer ✔Light emission produced due to enzymatic oxidation of a substrate
n n n n n n n n n n
Nephelometry is based on the measurement of light that is: - correct answer
n n n n n n n n n n n n
✔Scattered by particles in suspension
n n n n n
Which of the following instruments is used in the clinical lab or in reference
n n n n n n n n n n n n n n
labs to detect beta and gamma emissions? - correct answer ✔Scintillation
n n n n n n n n n n n
counter
Which of the following best describes chemiluminescence? - correct answer
n n n n n n n n n n
✔Chemical energy excites electrons that emit light upon return to ground
n n n n n n n n n n n
state
In assaying an analyte with a single beam atomic absorption
n n n n n n n n n n
spectrophotometer, what is the instrument actually measuring? - correct n n n n n n n n n
answer ✔Intensity of the beam from the hollow cathode lamp after it has
n n n n n n n n n n n n n
passed through the analyte containing flame
n n n n n
What is the function of the flame in atomic absorption spectroscopy? -
n n n n n n n n n n n n
correct answer ✔Bring the metal analyte to its ground state
n n n n n n n n n
, Most atomic absorption spectrophotometers incorporate a beam chopper
n n n n n n n n
and a tuned amplifier. The purpose of these components is to avoid errors
n n n n n n n n n n n n n
that would be caused by: - correct answer ✔Measurement of light emitted
n n n n n n n n n n n n
by the analyte
n n
In potentiometry, which of the following is considered the standard
n n n n n n n n n n
electrode? - correct answer ✔Hydrogen electrode
n n n n n
In an electrolytic cell, which of the following is the half cell where reduction
n n n n n n n n n n n n n n
takes place? - correct answer ✔Cathode
n n n n n
Mercury covered by a layer of mercurous chloride in contact with saturated
n n n n n n n n n n n n
potassium chloride solution is a description of which of the following types
n n n n n n n n n n n n
of electrodes? - correct answer ✔Calomel
n n n n n
When a pH sensitive glass electrode is not actively in use, in what type of
n n n n n n n n n n n n n n n
solution should it be kept? - correct answer ✔The medium recommended by
n n n n n n n n n n n
nthe manufacturer
n
When measuring K positive with an ion selective electrode by means of a
n n n n n n n n n n n n n
liquid ion exchange membrane, what antibiotic will be incorporated into the
n n n n n n n n n n n
membrane? - correct answer ✔Valinomycin n n n n
Which of the following is false about ion selective electrode analysis of
n n n n n n n n n n n n
sodium? - correct answer ✔Membrane coated with valinomycin
n n n n n n n
