, 1- Safety Precautions in the Microbiology
Laboratory
I – PPEs (personal protective equipment):
Wear a lab coat, rubber gloves, goggles, mask and foot wear while at work.
II-Personal Hygiene
Do not eat, drink or smoke in the lab.
Do not lick labels.
Do not put fingers, pencils or other objects into mouth.
Do not finger the eyes.
Wash hands with soap and water before leaving the lab.
Laboratory personnel should be vaccinated against infectious agents.
❖ Washing hands with water and soap or other detergents containing antiseptic agent
(more effective) But If visible dirt on the hands, you must wash with water and soap.
III- Safe work practice
Wipe benches with a disinfectant before and after work.
Never pipette by mouth.
Label all tubes, plates and any lab container.
Be aware of chemical hazards
Be careful near Bunsen flame
Treat all organisms as possibly infectious.
If dealing with a highly contagious organism work should be performed under a
laminar flow hood or glove box.
Biological safety
cabinet
Page | 1
,IV- Administrative control
Restrict access to authorized persons and lab personnel.
Do not take cultures out of the lab.
Keep doors always closed.
Report any accidents, spills, broken glass, cuts and injuries (needle sticks).
V-Adequate waste disposal
All biohazardous materials should be packed in such labeled containers.
Sharp contaminated objects (syringe, needles, broken glass) placed in a puncture
proof container (safety box) until disposal.
Waste disposal Bags
Use : Disposal of biological waste
Biohazard sign
Use : labeling of infectious
materials
Page | 2
, Safety Box
Use : Disposal of needles and
sharp objects
2- Microscope
Bacteria are very small in size, measured in term of microns (μ).
they cannot be seen by naked eye.
Microscope is an instrument for producing magnified and
resolved image of objects that are too small to be seen by the naked
eye.
Factors determine the quality of an optical image:
1-Magnification:
❖ The ability to increase the size of an image by using a convex lens.
2-Resolution:
❖ The ability to distinguish two neighboring points as separate entities
allowing the study of the structural details.
❖ Depends on the wavelength of the used source of illumination (shorter
wavelength → the greater the resolution).
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Laboratory
I – PPEs (personal protective equipment):
Wear a lab coat, rubber gloves, goggles, mask and foot wear while at work.
II-Personal Hygiene
Do not eat, drink or smoke in the lab.
Do not lick labels.
Do not put fingers, pencils or other objects into mouth.
Do not finger the eyes.
Wash hands with soap and water before leaving the lab.
Laboratory personnel should be vaccinated against infectious agents.
❖ Washing hands with water and soap or other detergents containing antiseptic agent
(more effective) But If visible dirt on the hands, you must wash with water and soap.
III- Safe work practice
Wipe benches with a disinfectant before and after work.
Never pipette by mouth.
Label all tubes, plates and any lab container.
Be aware of chemical hazards
Be careful near Bunsen flame
Treat all organisms as possibly infectious.
If dealing with a highly contagious organism work should be performed under a
laminar flow hood or glove box.
Biological safety
cabinet
Page | 1
,IV- Administrative control
Restrict access to authorized persons and lab personnel.
Do not take cultures out of the lab.
Keep doors always closed.
Report any accidents, spills, broken glass, cuts and injuries (needle sticks).
V-Adequate waste disposal
All biohazardous materials should be packed in such labeled containers.
Sharp contaminated objects (syringe, needles, broken glass) placed in a puncture
proof container (safety box) until disposal.
Waste disposal Bags
Use : Disposal of biological waste
Biohazard sign
Use : labeling of infectious
materials
Page | 2
, Safety Box
Use : Disposal of needles and
sharp objects
2- Microscope
Bacteria are very small in size, measured in term of microns (μ).
they cannot be seen by naked eye.
Microscope is an instrument for producing magnified and
resolved image of objects that are too small to be seen by the naked
eye.
Factors determine the quality of an optical image:
1-Magnification:
❖ The ability to increase the size of an image by using a convex lens.
2-Resolution:
❖ The ability to distinguish two neighboring points as separate entities
allowing the study of the structural details.
❖ Depends on the wavelength of the used source of illumination (shorter
wavelength → the greater the resolution).
Page | 3
