MICR290 - FINAL STUDY GUIDE
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1. Which of the following prokaryotic cells possesses an outer membrane?: -
Gram-negative bacteria
2. Free-living microorganisms characterized by the absence of a nucleus are
called:: Prokaryotes
3. Using a microscope, you observe an amoeba moving toward a food source.
This is an example of:: Chemotaxis
4. Which of the following is an incorrect pairing?
Fungi - yeast
Protozoa - prokaryote
Algae - multicellular
Viruses - acellular
Bacteria- unicellular: protozoa-prokaryote
5. The process by which parts of DNA getting copied to make messenger RNA
is called:: Transcription
6. Presence of this structure in spiral shaped bacteria (spirochetes) gives
them penetrating power into the tissues.: Axial filament
7. The cell walls of __________ contain peptidoglycan.: Bacteria
8. Which of the following organelles is responsible for producing most of the
ATP in a eukaryotic cell?: Mitochondria
9. Which of the following bacterial cell structures plays an important role in
the creation of biofilms?: Capsule (Slime layer)
10. In bacteria, transfer of DNA from one cell to another by way of sex pilus is
called:: Conjugation
11. Which of the following structures are present in both eukaryotic and
prokaryotic cells?: Ribosomes
12. MacConkey agar plates represent ________ medium.: both selective and
differential
13. What is a virus that infects bacteria called?: phage
14. What is the correct order for the application of Koch's postulates?
I. Inoculate suspect agent into test subject and observe that subject develops
disease of interest.
II. Isolate and culture suspect agent in the laboratory.
III. Find suspect agent is every case of disease of interest but not in healthy
hosts.
IV. Recover and isolate suspect agent from test subject.: III. Find suspect agent
is every case of disease of interest but not in healthy hosts.
II. Isolate and culture suspect agent in the laboratory.
I. Inoculate suspect agent into test subject and observe that subject develops
disease of interest.
, MICR290 - FINAL STUDY GUIDE
Study online at https://quizlet.com/_bt4uz0
IV. Recover and isolate suspect agent from test subject.
(III, II, I, IV)
15. Bacteria reproduce by the process of:: Binary fission
16. Process of fermentation in bacteria may include generation of each of the
following molecules except:: Amino acid and Glucose
17. Which of the following scientists discovered microscope?: Leuwenhoek
18. Heterotrophic organisms create energy by which of the following routes?-
: Catabolism of nutrients
19. Which of the following is considered as anabolic reaction?: Protein synthe-
sis
20. Which one of the following microorganisms are best viewed by method of
dark field microscopy?: Spirochetes
21. Differences in quantity of which of the following components of bacterial
cell wall contribute most to the differences in the Gram reactivity following a
Gram stain?: Peptidoglycan
22. Assume the diameter of the field of vision in your microscope is 2.5 mm
under low power. If one yeast cell is 5 um, how many yeast cells could fit across
the field?: 500
23. Which one of the following steps of the Gram stain is called the differential
step?: Decolorization
24. A(n) ________ organism is one that dies in the presence of oxygen: Oblig-
ate anaerobe
25. Given that the drug penicillin destroys bacteria most effectively when they
are actively growing, during which phase of their growth curve, bacteria are
most susceptible to penicillin?: Exponential (Log) phase
26. All of the following structures may be found in eukaryotic cells except:: -
Lipopolysaccharide
27. A halophilic microbe thrives under conditions of higher: Salt concentrations
28. Which of the following enzymes is absent in microbes that are obligate
anaerobes?: Both Superoxide dismutase and Catalase
29. Most pathogenic bacteria are: Mesophiles and Neutrophiles
30. Serial 1:10 dilutions of a bacterial culture was prepared in the laboratory
and 0.1 ml of each dilution was plated out in the nutrient agar petri plates and
incubated for 24 hours. The colony forming units (CFU) count of the 1: 1000
diluted sample came to be 58. How many bacteria were present in the original
undiluted sample?: 5.8 x 10^5 cells/ml
31. In prokaryotic cells, aerobic respiration of one molecule of glucose will
give rise to net total of how many ATPs?: 38
Study online at https://quizlet.com/_bt4uz0
1. Which of the following prokaryotic cells possesses an outer membrane?: -
Gram-negative bacteria
2. Free-living microorganisms characterized by the absence of a nucleus are
called:: Prokaryotes
3. Using a microscope, you observe an amoeba moving toward a food source.
This is an example of:: Chemotaxis
4. Which of the following is an incorrect pairing?
Fungi - yeast
Protozoa - prokaryote
Algae - multicellular
Viruses - acellular
Bacteria- unicellular: protozoa-prokaryote
5. The process by which parts of DNA getting copied to make messenger RNA
is called:: Transcription
6. Presence of this structure in spiral shaped bacteria (spirochetes) gives
them penetrating power into the tissues.: Axial filament
7. The cell walls of __________ contain peptidoglycan.: Bacteria
8. Which of the following organelles is responsible for producing most of the
ATP in a eukaryotic cell?: Mitochondria
9. Which of the following bacterial cell structures plays an important role in
the creation of biofilms?: Capsule (Slime layer)
10. In bacteria, transfer of DNA from one cell to another by way of sex pilus is
called:: Conjugation
11. Which of the following structures are present in both eukaryotic and
prokaryotic cells?: Ribosomes
12. MacConkey agar plates represent ________ medium.: both selective and
differential
13. What is a virus that infects bacteria called?: phage
14. What is the correct order for the application of Koch's postulates?
I. Inoculate suspect agent into test subject and observe that subject develops
disease of interest.
II. Isolate and culture suspect agent in the laboratory.
III. Find suspect agent is every case of disease of interest but not in healthy
hosts.
IV. Recover and isolate suspect agent from test subject.: III. Find suspect agent
is every case of disease of interest but not in healthy hosts.
II. Isolate and culture suspect agent in the laboratory.
I. Inoculate suspect agent into test subject and observe that subject develops
disease of interest.
, MICR290 - FINAL STUDY GUIDE
Study online at https://quizlet.com/_bt4uz0
IV. Recover and isolate suspect agent from test subject.
(III, II, I, IV)
15. Bacteria reproduce by the process of:: Binary fission
16. Process of fermentation in bacteria may include generation of each of the
following molecules except:: Amino acid and Glucose
17. Which of the following scientists discovered microscope?: Leuwenhoek
18. Heterotrophic organisms create energy by which of the following routes?-
: Catabolism of nutrients
19. Which of the following is considered as anabolic reaction?: Protein synthe-
sis
20. Which one of the following microorganisms are best viewed by method of
dark field microscopy?: Spirochetes
21. Differences in quantity of which of the following components of bacterial
cell wall contribute most to the differences in the Gram reactivity following a
Gram stain?: Peptidoglycan
22. Assume the diameter of the field of vision in your microscope is 2.5 mm
under low power. If one yeast cell is 5 um, how many yeast cells could fit across
the field?: 500
23. Which one of the following steps of the Gram stain is called the differential
step?: Decolorization
24. A(n) ________ organism is one that dies in the presence of oxygen: Oblig-
ate anaerobe
25. Given that the drug penicillin destroys bacteria most effectively when they
are actively growing, during which phase of their growth curve, bacteria are
most susceptible to penicillin?: Exponential (Log) phase
26. All of the following structures may be found in eukaryotic cells except:: -
Lipopolysaccharide
27. A halophilic microbe thrives under conditions of higher: Salt concentrations
28. Which of the following enzymes is absent in microbes that are obligate
anaerobes?: Both Superoxide dismutase and Catalase
29. Most pathogenic bacteria are: Mesophiles and Neutrophiles
30. Serial 1:10 dilutions of a bacterial culture was prepared in the laboratory
and 0.1 ml of each dilution was plated out in the nutrient agar petri plates and
incubated for 24 hours. The colony forming units (CFU) count of the 1: 1000
diluted sample came to be 58. How many bacteria were present in the original
undiluted sample?: 5.8 x 10^5 cells/ml
31. In prokaryotic cells, aerobic respiration of one molecule of glucose will
give rise to net total of how many ATPs?: 38