MCB 2050 Midterm Review (Answered)
2025/2026. 100% Verified. 117Q&A.
What is the generic name of enzymes that recognize palindromic sequences in dsDNA and cleave the
DNA at that location?
Restriction endonucleases
The kind of ends following restriction endonuclease digestion that results in single stranded DNA
overhangs are called:
Cohesive (or sticky) ends
DNA fragments with compatible overhangs that have annealed together can be joined with this
enzyme:
DNA ligase
The name of the part of a cloning vector that has several restriction enzyme sites is called:
Multiple cloning site
In the Bluescript cloning plasmid, the MCS is located within the reading frame of what gene?
LacZ
Bluescript plasmids containing foreign DNA inserted in the MCS result in insertional inactivation.
What is the colour of bacteria harbouring recombinant plasmids when grown on agar plates
containing X-gal?
white
The kind of vector you need to produce a foreign protein is called:
Expression vector
A library made from cellular mRNA is called:
cDNA library
The process of amplifying DNA from a small amount using primer pairs is called:
Polymerase chain reaction (PCR)
The process of amplifying cDNA from a small amount of mRNA is called:
Reverse transcriptase polymerase chain reaction
The name of the enzyme used in PCR for strand elongation (extension) and resistant to high
temperatures is:
Taq DNA polymerase
The name of sequence specific single stranded DNAs used to initiate strand elongation in PCR are
called:
, Primers
The PCR technique used to measure mRNA abundance is called:
RT-PCR
The technique used to identify a specific DNA band following electrophoresis in an agarose gel is
called:
Southern blot
The technique used to identify a specific RNA band following electrophoresis in an agarose or
polyacrylamide gel is called:
Northern blot
The process by which a specific DNA or RNA on a membrane is detected using a radioactively
labelled DNA probe is called:
Molecular hybridization
Which technique for mRNA quantification is more rapid and quantifiable, Northern blotting, RT-PCR
or qRT-PCR?
qRT-PCR
The name of the process where you align restriction endonuclease sites to specific and accurate
locations on a piece of DNA is called:
Restriction mapping
If a DNA sample is cut with a restriction enzyme and produced two fragments whose sizes equal the
total size of the original DNA sample, was the DNA sample linear or circular before it was cut?
Linear
Why are dideoxynucleotides used in the Sanger sequencing reaction?
Incorporation terminates DNA synthesis producing DNA fragments differing in size by 1 or more
nucleotides
The technique used to separate proteins by size in a gel matrix is called:
SDS-PAGE
The technique used to identify a specific protein band following electrophoresis in a polyacrylamide
gel:
Western blotting
The technique used to study protein localization in fixed cells is called:
Immunofluorescence
What is the name of a fluorescent protein that is often fused to a protein of interest to follow the
localization of the protein of interest?
Green fluorescent protein (GFP)
2025/2026. 100% Verified. 117Q&A.
What is the generic name of enzymes that recognize palindromic sequences in dsDNA and cleave the
DNA at that location?
Restriction endonucleases
The kind of ends following restriction endonuclease digestion that results in single stranded DNA
overhangs are called:
Cohesive (or sticky) ends
DNA fragments with compatible overhangs that have annealed together can be joined with this
enzyme:
DNA ligase
The name of the part of a cloning vector that has several restriction enzyme sites is called:
Multiple cloning site
In the Bluescript cloning plasmid, the MCS is located within the reading frame of what gene?
LacZ
Bluescript plasmids containing foreign DNA inserted in the MCS result in insertional inactivation.
What is the colour of bacteria harbouring recombinant plasmids when grown on agar plates
containing X-gal?
white
The kind of vector you need to produce a foreign protein is called:
Expression vector
A library made from cellular mRNA is called:
cDNA library
The process of amplifying DNA from a small amount using primer pairs is called:
Polymerase chain reaction (PCR)
The process of amplifying cDNA from a small amount of mRNA is called:
Reverse transcriptase polymerase chain reaction
The name of the enzyme used in PCR for strand elongation (extension) and resistant to high
temperatures is:
Taq DNA polymerase
The name of sequence specific single stranded DNAs used to initiate strand elongation in PCR are
called:
, Primers
The PCR technique used to measure mRNA abundance is called:
RT-PCR
The technique used to identify a specific DNA band following electrophoresis in an agarose gel is
called:
Southern blot
The technique used to identify a specific RNA band following electrophoresis in an agarose or
polyacrylamide gel is called:
Northern blot
The process by which a specific DNA or RNA on a membrane is detected using a radioactively
labelled DNA probe is called:
Molecular hybridization
Which technique for mRNA quantification is more rapid and quantifiable, Northern blotting, RT-PCR
or qRT-PCR?
qRT-PCR
The name of the process where you align restriction endonuclease sites to specific and accurate
locations on a piece of DNA is called:
Restriction mapping
If a DNA sample is cut with a restriction enzyme and produced two fragments whose sizes equal the
total size of the original DNA sample, was the DNA sample linear or circular before it was cut?
Linear
Why are dideoxynucleotides used in the Sanger sequencing reaction?
Incorporation terminates DNA synthesis producing DNA fragments differing in size by 1 or more
nucleotides
The technique used to separate proteins by size in a gel matrix is called:
SDS-PAGE
The technique used to identify a specific protein band following electrophoresis in a polyacrylamide
gel:
Western blotting
The technique used to study protein localization in fixed cells is called:
Immunofluorescence
What is the name of a fluorescent protein that is often fused to a protein of interest to follow the
localization of the protein of interest?
Green fluorescent protein (GFP)
