LS7A Midterm 2 Exam Questions with Correct Answers Latest Update 2025

LS7A Midterm 2 Exam Questions with Correct Answers Latest Update 2025 RNA polymerase reads - Answers 3' to 5' polyA tail will be added at __________of the mRNA transcribed - Answers the end As RNA is synthesized, ribonucleotides are added to the ___ end of the growing RNA molecules - Answers 5' end This single celled eukaryotic organism only uses glucose as its source of energy. This cell does not contain ________________. You add a toxin to this cell that prevents pyruvate from getting into the ___________________ - Answers chloroplasts; mitochondria Cells treated with a toxin would use ______________ even if the ______________ is present - Answers fermentation; oxygen Pyruvate will not be __________ in cells treated with the toxin - Answers oxidized Cells treated with the toxin would produce _________ carbon dioxide - Answers less FAD will _______________________ in cells treated with the toxin - Answers accumulate If oxygen becomes unavailable in cellular respiration, the concentration of FeS2ox will_____________ over time - Answers decrease If oxygen becomes unavailable, the rate of acetyl-CoA production will ________________ over time - Answers decrease If the redox potential of Cyt b changes to +170 mV, the concentration of Cyt aox, will eventually __________ - Answers increase Artificially decreasing the concentration of H+ in the matrix will cause ATP production to ____________ - Answers increase As the total amount of ATP increases in a cell the total amount of ADP in the cell will ______________ - Answers decrease Operons: Mutation C* affects the CRP protein binding site. The CRP protein can bind this mutated binding site and activate transcription regarding whether or not it's bound to cAMP. To study the effects of this mutation, you grow E. Coli cells with the lac operon type I+C*O+Z+ under three conditions. Identify the lac operon expression -Lactose as the only food source causes ________expression Glucose as the only food source __________ expression Glucose and lactose as the only food source causes ________________expression - Answers high; no; high Low glucose, _____cAMP - Answers high ___________ glucose, low cAMP - Answers high CRP-cAMP binds to site near __________ activating ____________ - Answers promoter; transcription If CRP-cAMP doesn't bind, there will be no _____________, even with lactose - Answers transcription The lactose operon is under ________