BCHM 4360 EXAM 2 NEWEST 2025 ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW!!
What is the next step in transcription initiation after TFIIB has bound? -
ANSWER--TFIIA binds and stabilizes the TBP-DNA interactions.
What is the next step in transcription initiation after TFIIA binds? -
ANSWER--TFIIE and TFIIH bind, which catalyze ATP-powered unwinding
of DNA
What are the two main components of the pre-inititation complex? -
ANSWER--RNA Polymerase II and general transcription factors
True or False: The pre-initiation complex is good enough to initiate
transcription in vitro and in vivo. - ANSWER--False, in vivo requires
additional protein complexes
Another large complex, ________, is needed to activate many
Polymerase II transcribed genes. - ANSWER--mediator
What are the main similarities between Pol I, II, and III? - ANSWER--
Core subunits are similar, all three use TBP to initiate transcription
, BCHM 4360 EXAM 2 NEWEST 2025 ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW!!
What are the main differences between Pol I, II, and III? - ANSWER--TBP
is part of different complexes in each enzyme, each use different
proteins to initiate transcription, recognize different promoters, and
have different general transcription factors
Once RNA Polymerase is in position, the complex of polymerase and
promoter is called a ______ complex. - ANSWER--closed
Approximatel how many bp of duplex DNA (transcription bubble) does
RNA Polymerase open up? - ANSWER--14bp
True or False: ATP is required to open up the transcription bubble in all
three RNA Polymerases - ANSWER--False, only Pol II requires ATP
The RNA Polymerase bound to an open region of DNA is called the ____
complex. - ANSWER--open
RNA polymerase frequently fails to make a full length RNA on the first
attempt, which leads to the release of short RNAs of 2-9 nucleotides,
and is called ________ initiation. - ANSWER--abortive
, BCHM 4360 EXAM 2 NEWEST 2025 ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW!!
______________ is the key to release the tether attached to RNA
Polymerase so that RNA Polymerase can get going like it wants to. -
ANSWER--phosphorylation
The non-template strand of DNA is held apart by which three regions of
RNA Polymerase? - ANSWER--lid, zipper, and rudder regions
Successive nucleotides are added at the __ end of the growing RNA
molecule by ____________ attack, forming a _____________ bond and
releasing ____________. - ANSWER--3', nucleophilic, phosphodiester,
pyrophosphate
The __ end of the growing RNA leaves the polymerase via an ____
_______. - ANSWER--5', exit channel
Just like DNA Polymerase, which ions are present at the active site and
catalyze the addition of nucleotides? - ANSWER--magnesium
How do magnesium ions work with RNA Polymerase? - ANSWER--
activate the 3' OH and stabilize a negative charge on the leaving oxygen
, BCHM 4360 EXAM 2 NEWEST 2025 ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW!!
Bacterial _____ ______ and eukaryotic ____ are involved in abortive
initiation. Both proteins have a ____ that extends into the RNA
Polymerase active site region - ANSWER--sigma factor, TFIIB, loop
How does the loop in abortive initiation function? - ANSWER--It is in
position to block the elongating transcript, so the loop must be moved
in order for transcription to continue. Displacement of the loop is
thought to help the polymerase break away from the promoter -
promoter clearance
During promoter clearance, RNA Polymerase undergoes a
____________ change that associates it very ______ with DNA, and
_______ its grip on initiation factors. - ANSWER--conformational,
stably, loosens
What is characteristic of the switch from initiation to elongation? -
ANSWER--Dissociation of the general transcription initiation factors
RNA Polymerase remains associated with DNA and adds _________ or
________ of bases to growing RNA at about __-__ nucleotides per
second. - ANSWER--hundreds, thousands, 20-50
EXAM COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW!!
What is the next step in transcription initiation after TFIIB has bound? -
ANSWER--TFIIA binds and stabilizes the TBP-DNA interactions.
What is the next step in transcription initiation after TFIIA binds? -
ANSWER--TFIIE and TFIIH bind, which catalyze ATP-powered unwinding
of DNA
What are the two main components of the pre-inititation complex? -
ANSWER--RNA Polymerase II and general transcription factors
True or False: The pre-initiation complex is good enough to initiate
transcription in vitro and in vivo. - ANSWER--False, in vivo requires
additional protein complexes
Another large complex, ________, is needed to activate many
Polymerase II transcribed genes. - ANSWER--mediator
What are the main similarities between Pol I, II, and III? - ANSWER--
Core subunits are similar, all three use TBP to initiate transcription
, BCHM 4360 EXAM 2 NEWEST 2025 ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW!!
What are the main differences between Pol I, II, and III? - ANSWER--TBP
is part of different complexes in each enzyme, each use different
proteins to initiate transcription, recognize different promoters, and
have different general transcription factors
Once RNA Polymerase is in position, the complex of polymerase and
promoter is called a ______ complex. - ANSWER--closed
Approximatel how many bp of duplex DNA (transcription bubble) does
RNA Polymerase open up? - ANSWER--14bp
True or False: ATP is required to open up the transcription bubble in all
three RNA Polymerases - ANSWER--False, only Pol II requires ATP
The RNA Polymerase bound to an open region of DNA is called the ____
complex. - ANSWER--open
RNA polymerase frequently fails to make a full length RNA on the first
attempt, which leads to the release of short RNAs of 2-9 nucleotides,
and is called ________ initiation. - ANSWER--abortive
, BCHM 4360 EXAM 2 NEWEST 2025 ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW!!
______________ is the key to release the tether attached to RNA
Polymerase so that RNA Polymerase can get going like it wants to. -
ANSWER--phosphorylation
The non-template strand of DNA is held apart by which three regions of
RNA Polymerase? - ANSWER--lid, zipper, and rudder regions
Successive nucleotides are added at the __ end of the growing RNA
molecule by ____________ attack, forming a _____________ bond and
releasing ____________. - ANSWER--3', nucleophilic, phosphodiester,
pyrophosphate
The __ end of the growing RNA leaves the polymerase via an ____
_______. - ANSWER--5', exit channel
Just like DNA Polymerase, which ions are present at the active site and
catalyze the addition of nucleotides? - ANSWER--magnesium
How do magnesium ions work with RNA Polymerase? - ANSWER--
activate the 3' OH and stabilize a negative charge on the leaving oxygen
, BCHM 4360 EXAM 2 NEWEST 2025 ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
DETAILED ANSWERS (VERIFIED ANSWERS)
|ALREADY GRADED A+||BRAND NEW!!
Bacterial _____ ______ and eukaryotic ____ are involved in abortive
initiation. Both proteins have a ____ that extends into the RNA
Polymerase active site region - ANSWER--sigma factor, TFIIB, loop
How does the loop in abortive initiation function? - ANSWER--It is in
position to block the elongating transcript, so the loop must be moved
in order for transcription to continue. Displacement of the loop is
thought to help the polymerase break away from the promoter -
promoter clearance
During promoter clearance, RNA Polymerase undergoes a
____________ change that associates it very ______ with DNA, and
_______ its grip on initiation factors. - ANSWER--conformational,
stably, loosens
What is characteristic of the switch from initiation to elongation? -
ANSWER--Dissociation of the general transcription initiation factors
RNA Polymerase remains associated with DNA and adds _________ or
________ of bases to growing RNA at about __-__ nucleotides per
second. - ANSWER--hundreds, thousands, 20-50
