(MB) ASCP Practice Exam Questions an
m m m m m
d Answers Solved Correctly
m m m
Whichmof mthemfollowingmismnot mamcomponent mof mamnucleotid
e?mPhosphatemgroup
Anti-
codonmRibos
emsugar
Nitrogenmbasem✔✔Anti-codon
AccordingmtomChargaff'smrulemof mbasempairing,madeninempairsmwith:m✔✔Thymine
What mgenesmwould mbemscreened minmambreast mcancermpanel?m✔✔HER2,mERBB2,mBRCA1
Next mGenerationmSequencingmuses:m✔✔Short msequencemreads
Purinesm and m pyrimidinesm differm fromm eachm otherm inm that:m ✔✔Purinesm havem twom rings;m pyrimidines
havemonemring
Thempurinesmare:
Cytosinemand muracilmAdeni
nemand mthyminemThyminema
nd mcytosine
Adeninemand mguaninem✔✔Adeninemand mguanine
,What mismthemratemof mmutationmpermround mof mDNA mreplicatio
n?m1minm1,000mbasempairs
1minm10,000mbasempairs
1minm1,000,000mbasempairs
1minm1,000,000,000mbasempairsm✔✔1minm1,000,000,000mbasempairs
Them ratem of m DNA m migrationm throughm anm agarosem gelm duringm electrophoresism doesm not m depend m onm
whichmof mthemfollowingmfactors?
Net mchargemof mthemmolecul
emSizemof mthemmoleculem Sh
apemof mthemmolecule
Nucleotidemsequencemof mthemmoleculem✔✔Nucleotidemsequencemof mthemmolecule
What maremthemphasesminmamqPCRmAmplificationmPlot?mInitiat
ion,mexponential,mplateau
Baseline,mexponential,mplateau
Baseline,mthreshold,mexponential,mplateau
Baseline,minitiation,mthreshold,mexponential,mplateaum✔✔Initiation,mexponential,mplateau
Find mthempalindromeminmthismrestrictionmenzymemsite:m5'-CTGCAG-3'?
5'-GAC
,3'-GAC
3'-CTG
5'-GTCm✔✔3'-GAC
Ampatient mwithmimpaired mjudgment,mpersonalitymchanges,msignsmof mabnormalmbodymmovementsman
d mdepressionmcomesmtomthemphysician'smofficemformamfollow-
upmvisit.m Themphysician msuspectsmam single-
genemdisordermmaymbemthemcausemof mthosemclinical mmanifestations.mA mblood mspecimenmwasmthenms
m
ent mtomyourmclinicalm laboratorymformmutationmscreening minmthemHuntingtonmgene.mTestingmwithmsta
ndard mPCRmindicatesmthat mpatient mhasmHuntingtonmDisease,mHD.mWhichmof mthemfollowingmwould m
bemconsistent mwithmthismdiagnosis?
25mCAGmrepeatsminmthemHuntingtonmgen
em85mCAG mrepeatsminmthemHuntingtonmg
enem25mCGA mrepeatsminmthemHuntington
gene
m
85mCGA mrepeatsminmthemHuntingtonmgenem✔✔85mCAG mrepeatsminmthemHuntingtonmgene
WhichmtwomHPV mtypesmaremresponsiblemformmost mcasesmof mcervicalmcancer
?m16mand m18
31mand m59
16mand m58
44mand m59m✔✔16mand m18
Replicationmforks,mknownmasmoriginsmof mDNAmreplication,maremcreated mbymthismenzyme:mLi
gase
, TaqmPolymerase
Primase
m
Helicasem✔✔Helicase
Mutationminmwhat mgenemismassociated mwithmFragilemXmsyndrome?m✔✔FMR1
Mantlemcellmlymphomam(MCL)mismcaused mbymwhat mtranslocation?m✔✔t(11;14)
Thismpolymerasemisminvolved minm"initiationmof mDNA mreplicationmand mhasmprimasemactivity":m✔✔Pol mα
Itsmdiscoverymshed mlight monmwhymtheremismsimultaneous,mthoughmnot mcontinuous,msynthesismof mDN
Amonmbothmleadingmand mlaggingmstrandsmof mDNA:
Klenow mfragment mof mDNA mpolymerasemOkaza
kimfragments
Sangermfragments
RNA mfragmentsm✔✔Okazakimfragments
What mgenemismmeasured mfollowingmtreatment mwithmimatinibm(Gleevec)?mFL
T3
BCR/Abl
Jak2
m
MAPKm✔✔BCR/Abl
m m m m m
d Answers Solved Correctly
m m m
Whichmof mthemfollowingmismnot mamcomponent mof mamnucleotid
e?mPhosphatemgroup
Anti-
codonmRibos
emsugar
Nitrogenmbasem✔✔Anti-codon
AccordingmtomChargaff'smrulemof mbasempairing,madeninempairsmwith:m✔✔Thymine
What mgenesmwould mbemscreened minmambreast mcancermpanel?m✔✔HER2,mERBB2,mBRCA1
Next mGenerationmSequencingmuses:m✔✔Short msequencemreads
Purinesm and m pyrimidinesm differm fromm eachm otherm inm that:m ✔✔Purinesm havem twom rings;m pyrimidines
havemonemring
Thempurinesmare:
Cytosinemand muracilmAdeni
nemand mthyminemThyminema
nd mcytosine
Adeninemand mguaninem✔✔Adeninemand mguanine
,What mismthemratemof mmutationmpermround mof mDNA mreplicatio
n?m1minm1,000mbasempairs
1minm10,000mbasempairs
1minm1,000,000mbasempairs
1minm1,000,000,000mbasempairsm✔✔1minm1,000,000,000mbasempairs
Them ratem of m DNA m migrationm throughm anm agarosem gelm duringm electrophoresism doesm not m depend m onm
whichmof mthemfollowingmfactors?
Net mchargemof mthemmolecul
emSizemof mthemmoleculem Sh
apemof mthemmolecule
Nucleotidemsequencemof mthemmoleculem✔✔Nucleotidemsequencemof mthemmolecule
What maremthemphasesminmamqPCRmAmplificationmPlot?mInitiat
ion,mexponential,mplateau
Baseline,mexponential,mplateau
Baseline,mthreshold,mexponential,mplateau
Baseline,minitiation,mthreshold,mexponential,mplateaum✔✔Initiation,mexponential,mplateau
Find mthempalindromeminmthismrestrictionmenzymemsite:m5'-CTGCAG-3'?
5'-GAC
,3'-GAC
3'-CTG
5'-GTCm✔✔3'-GAC
Ampatient mwithmimpaired mjudgment,mpersonalitymchanges,msignsmof mabnormalmbodymmovementsman
d mdepressionmcomesmtomthemphysician'smofficemformamfollow-
upmvisit.m Themphysician msuspectsmam single-
genemdisordermmaymbemthemcausemof mthosemclinical mmanifestations.mA mblood mspecimenmwasmthenms
m
ent mtomyourmclinicalm laboratorymformmutationmscreening minmthemHuntingtonmgene.mTestingmwithmsta
ndard mPCRmindicatesmthat mpatient mhasmHuntingtonmDisease,mHD.mWhichmof mthemfollowingmwould m
bemconsistent mwithmthismdiagnosis?
25mCAGmrepeatsminmthemHuntingtonmgen
em85mCAG mrepeatsminmthemHuntingtonmg
enem25mCGA mrepeatsminmthemHuntington
gene
m
85mCGA mrepeatsminmthemHuntingtonmgenem✔✔85mCAG mrepeatsminmthemHuntingtonmgene
WhichmtwomHPV mtypesmaremresponsiblemformmost mcasesmof mcervicalmcancer
?m16mand m18
31mand m59
16mand m58
44mand m59m✔✔16mand m18
Replicationmforks,mknownmasmoriginsmof mDNAmreplication,maremcreated mbymthismenzyme:mLi
gase
, TaqmPolymerase
Primase
m
Helicasem✔✔Helicase
Mutationminmwhat mgenemismassociated mwithmFragilemXmsyndrome?m✔✔FMR1
Mantlemcellmlymphomam(MCL)mismcaused mbymwhat mtranslocation?m✔✔t(11;14)
Thismpolymerasemisminvolved minm"initiationmof mDNA mreplicationmand mhasmprimasemactivity":m✔✔Pol mα
Itsmdiscoverymshed mlight monmwhymtheremismsimultaneous,mthoughmnot mcontinuous,msynthesismof mDN
Amonmbothmleadingmand mlaggingmstrandsmof mDNA:
Klenow mfragment mof mDNA mpolymerasemOkaza
kimfragments
Sangermfragments
RNA mfragmentsm✔✔Okazakimfragments
What mgenemismmeasured mfollowingmtreatment mwithmimatinibm(Gleevec)?mFL
T3
BCR/Abl
Jak2
m
MAPKm✔✔BCR/Abl
