HCT Midterm Practicals 2025
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Exercise 1 Supplies & Equipment
Use: Thin Slicing of Tissue
Rotary Microtome
Maintain: Remove excess paraffin,
Apply oil in moving parts
Use: Frozen Sectioning
Cryostat Microtome
Maintain: Remove excess paraffin,
Temp. at -20 degrees
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, 3/27/25, 12:22 PM HCT Midterm Practicals 2025 update|comprehensive questions and verified answers (complete solutions) ASSURED SUCCE…
Use: Automated embedding &
cooling of tissues
Automated Tissue
Embedding System
Maintain: Temp. of cold plate,
Clean embedding mold
Use: Automatic tissue processing
Automated Tissue
Processor Maintain: Clean beakers,
Change sol'n once a week
Use: Microscopic examination of tissue
Binocular Light
Microscope
Maintain: Clean oil when using OIO
Use: Cutting tissue section
Microtome Knife w/
Adaptor
Maintain: Sharpen dull ends if reusable
Use: Holding & identifying tissues
Plastic Tissue Cassette
Maintain: Wash and dry,
Remove Excess Paraffin
Use: Tissue samples are mounted
Glass Slides w/ Cover for examination
Slips
Maintain: Disposable
Use: Tease folds in tissue
Teasing Needle / Probe
Maintain: Clean / wipe to prevent rusting
Use: Stretch sections,
remove wrinkles and folds
Tissue Floatation Bath
Maintain: Free from oil & albumin,
check temp: 45-50C
Use: Holding slides during staining
Staining Dish & Slide
Carrier Maintain: Clean with soap water,
Rinse with distilled water
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