Blood banking exam 1 |95 Questions
and Answers
A one step test that detects in vivo coating of an individuals red blood cells
with IgG or complement - -Direct Antiglobulin Test (DAT)
- When are positive DATs seen? - -Acute and delayed hemolytic transfusion
reactions, autoantibodies, and drug induced hemolytic events
- After getting a positive DAT how can it be evaluated further? - -Elution and
adsorption
- Removing an antibody that is attached to the surface of a red blood cell
(acidic glycine may be used) - -Elution
- Procedure to bind antibodies to red blood cell antigens in order to remove
antibodies from plasma and analyze antibodies that may remain behind - -
Adsorption
- An antibody formed in response to pregnancy or transfusion targeted
against an antigen that is not present on persons red blood cell - -
Alloantibody
- What is the anti-A1 lectin? - -Dolichos biflorus
- What is the anti-H lectin? - -Ulex europeaus
- An antibody that is targeted against antigens that are present on the
patient own red cells - -Autoantibody
- Check cells are red blood cells coated with what? - -IgG
- Done to check for the presence of antibody and/or complement coating red
cells following incubation with serum or plasma - -Indirect anti globulin test
- The IAT is ______ testing and requires a ______phase - -In vitro
Incubation phase
- DCe - -R1
- DcE - -R2
- DCE - -Rz
, - Dce - -R
- dCe - -r'
- dcE - -r2
- dCE - -ry
- dce - -r
- Gene that makes ABO precursor on the red cells - -FUT1
- Gene that makes precursor that makes soluble in body fluids - -FUT 2
- Gene encodes lewis antigens - -FUT3
- What is the name of the precursor substance from which A and B antigens
are made? - -H
- What is the terminal sugar on this precursor? - -Fucose
- What sugar must be added to the precursor for A antigens to be
expressed? - -N- acetylgalactosamine
- What sugar must be added to the precursor for B antigens to be
expressed? - -galactose
- When are A and B antigens first seen? - -5-6 weeks of gestation
- When should people have their adult antigen set? - -3-4 years old
- A and B are _____ while O is an _____ - -codominant, amorph
- What are the weak sub groups of A and their occurrence? - -A1, A2, A3,
Ax, Am, Ael
- How would you detect Ax? - -Use monoclonal antibodies
- How would you detect Ael? - -By doing an elution
- ____ and _____ are capable of glycosalating different structures that are
more complicated - -A1 and A1B
- ________ can only glycosalate simple structures - -subgroups of A
and Answers
A one step test that detects in vivo coating of an individuals red blood cells
with IgG or complement - -Direct Antiglobulin Test (DAT)
- When are positive DATs seen? - -Acute and delayed hemolytic transfusion
reactions, autoantibodies, and drug induced hemolytic events
- After getting a positive DAT how can it be evaluated further? - -Elution and
adsorption
- Removing an antibody that is attached to the surface of a red blood cell
(acidic glycine may be used) - -Elution
- Procedure to bind antibodies to red blood cell antigens in order to remove
antibodies from plasma and analyze antibodies that may remain behind - -
Adsorption
- An antibody formed in response to pregnancy or transfusion targeted
against an antigen that is not present on persons red blood cell - -
Alloantibody
- What is the anti-A1 lectin? - -Dolichos biflorus
- What is the anti-H lectin? - -Ulex europeaus
- An antibody that is targeted against antigens that are present on the
patient own red cells - -Autoantibody
- Check cells are red blood cells coated with what? - -IgG
- Done to check for the presence of antibody and/or complement coating red
cells following incubation with serum or plasma - -Indirect anti globulin test
- The IAT is ______ testing and requires a ______phase - -In vitro
Incubation phase
- DCe - -R1
- DcE - -R2
- DCE - -Rz
, - Dce - -R
- dCe - -r'
- dcE - -r2
- dCE - -ry
- dce - -r
- Gene that makes ABO precursor on the red cells - -FUT1
- Gene that makes precursor that makes soluble in body fluids - -FUT 2
- Gene encodes lewis antigens - -FUT3
- What is the name of the precursor substance from which A and B antigens
are made? - -H
- What is the terminal sugar on this precursor? - -Fucose
- What sugar must be added to the precursor for A antigens to be
expressed? - -N- acetylgalactosamine
- What sugar must be added to the precursor for B antigens to be
expressed? - -galactose
- When are A and B antigens first seen? - -5-6 weeks of gestation
- When should people have their adult antigen set? - -3-4 years old
- A and B are _____ while O is an _____ - -codominant, amorph
- What are the weak sub groups of A and their occurrence? - -A1, A2, A3,
Ax, Am, Ael
- How would you detect Ax? - -Use monoclonal antibodies
- How would you detect Ael? - -By doing an elution
- ____ and _____ are capable of glycosalating different structures that are
more complicated - -A1 and A1B
- ________ can only glycosalate simple structures - -subgroups of A
