Bio322
Bio322 Midterm Exam Questions and
Answers 2025
Pipetmans measure what amount of volume? -Correct Answer ✔1 ml (1000 ul)
What measurements of pipette we have? -Correct Answer ✔P2, P20, P200, P1000
True/ False: There are only 3 places on the readout window of all pipet mans. Red
places are decimals or commas. -Correct Answer ✔True
True/ False: Hold pipet horizontally, insert the correct pipet tip to the shaft. Press firmly
but not pound> -Correct Answer ✔False: Hold it vertically
Genomic DNA constitutes _________ of an organism. -Correct Answer ✔Total genetic
information
What is the genome of most of the organism? -Correct Answer ✔DNA , some viruses
have RNA as their genomic material
What are the genomic DNA material known as? -Correct Answer ✔Chromosome
Chromosomes are organized into? -Correct Answer ✔DNA- protein complex
Viral DNA genomes are...... -Correct Answer ✔Small and single stranded
Where is most genomic DNA is located? -Correct Answer ✔Within the nucleus
What is the location of DNA in Eukaryotes? -Correct Answer ✔nucleus, mitochondria,
and chloroplasts in plants.
Genomic DNA contains..... -Correct Answer ✔Genes
A gene comprises the? -Correct Answer ✔-Coding region
- regulatory elements for controlling gene expressions.
What is the function of nucleases? -Correct Answer ✔cleave the phosphodiester bond
in the DNA. It degrade the DNA.
What is the function of nucleases? -Correct Answer ✔Degrading to DNA sequence to
gain protection against viruses.
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, Bio322
DNA is subjected to ________ when stored in water, and should therefore be stored in
____________. -Correct Answer ✔acid hydrolysis, TE buffere
Where you should store your DNA at all the time? -Correct Answer ✔On Ice, this with
prevent in degradation of the DNA.
What is the process of Lysing? -Correct Answer ✔Breaking down of the DNA.
What is the plus point of aseptic and sterile technique? -Correct Answer ✔protecting the
DNA from contamination.
What are some aseptic techniques? -Correct Answer ✔- always start with sterile
materials.
- always clean your work bench
-always wash your hands.
-Always use fresh pipette tip and change between every sample.
- Do not let your pipette touch anything.
-After getting a tip on the pipette, close tip box.
Don't touch insides of any of your tube, keep tubes closed at all times.
What you should label on your tube? -Correct Answer ✔Name, Date, and What is
Inside.
Why do you want to freeze an insect before extracting the DNA? -Correct Answer ✔-
For preservation of DNA integrity
-Inactivation of enzymes
Facilitation of tissue disruption
-Convenience and flexibility
Why did we used CTL buffer? -Correct Answer ✔It help in lysing process
Why did we used the Proteinase K? -Correct Answer ✔Because it breakdown the
protein.
Why do we vortex? -Correct Answer ✔To mix things
why is chloroform:isoamyl ratio is important? -Correct Answer ✔It purified the DNA by
separating DNA from cellular components, denaturing proteins, inactivating nucleases,
and removing contaminants.
Why do we centrifuge the material? -Correct Answer ✔We centrifuge the material for
the separation, concentrations and purification of biological and chemical samples.
What measurement do we use to centrifuge? -Correct Answer ✔10,000 G (RCF)
Bio322
Bio322 Midterm Exam Questions and
Answers 2025
Pipetmans measure what amount of volume? -Correct Answer ✔1 ml (1000 ul)
What measurements of pipette we have? -Correct Answer ✔P2, P20, P200, P1000
True/ False: There are only 3 places on the readout window of all pipet mans. Red
places are decimals or commas. -Correct Answer ✔True
True/ False: Hold pipet horizontally, insert the correct pipet tip to the shaft. Press firmly
but not pound> -Correct Answer ✔False: Hold it vertically
Genomic DNA constitutes _________ of an organism. -Correct Answer ✔Total genetic
information
What is the genome of most of the organism? -Correct Answer ✔DNA , some viruses
have RNA as their genomic material
What are the genomic DNA material known as? -Correct Answer ✔Chromosome
Chromosomes are organized into? -Correct Answer ✔DNA- protein complex
Viral DNA genomes are...... -Correct Answer ✔Small and single stranded
Where is most genomic DNA is located? -Correct Answer ✔Within the nucleus
What is the location of DNA in Eukaryotes? -Correct Answer ✔nucleus, mitochondria,
and chloroplasts in plants.
Genomic DNA contains..... -Correct Answer ✔Genes
A gene comprises the? -Correct Answer ✔-Coding region
- regulatory elements for controlling gene expressions.
What is the function of nucleases? -Correct Answer ✔cleave the phosphodiester bond
in the DNA. It degrade the DNA.
What is the function of nucleases? -Correct Answer ✔Degrading to DNA sequence to
gain protection against viruses.
Bio322
, Bio322
DNA is subjected to ________ when stored in water, and should therefore be stored in
____________. -Correct Answer ✔acid hydrolysis, TE buffere
Where you should store your DNA at all the time? -Correct Answer ✔On Ice, this with
prevent in degradation of the DNA.
What is the process of Lysing? -Correct Answer ✔Breaking down of the DNA.
What is the plus point of aseptic and sterile technique? -Correct Answer ✔protecting the
DNA from contamination.
What are some aseptic techniques? -Correct Answer ✔- always start with sterile
materials.
- always clean your work bench
-always wash your hands.
-Always use fresh pipette tip and change between every sample.
- Do not let your pipette touch anything.
-After getting a tip on the pipette, close tip box.
Don't touch insides of any of your tube, keep tubes closed at all times.
What you should label on your tube? -Correct Answer ✔Name, Date, and What is
Inside.
Why do you want to freeze an insect before extracting the DNA? -Correct Answer ✔-
For preservation of DNA integrity
-Inactivation of enzymes
Facilitation of tissue disruption
-Convenience and flexibility
Why did we used CTL buffer? -Correct Answer ✔It help in lysing process
Why did we used the Proteinase K? -Correct Answer ✔Because it breakdown the
protein.
Why do we vortex? -Correct Answer ✔To mix things
why is chloroform:isoamyl ratio is important? -Correct Answer ✔It purified the DNA by
separating DNA from cellular components, denaturing proteins, inactivating nucleases,
and removing contaminants.
Why do we centrifuge the material? -Correct Answer ✔We centrifuge the material for
the separation, concentrations and purification of biological and chemical samples.
What measurement do we use to centrifuge? -Correct Answer ✔10,000 G (RCF)
Bio322
