SBB Human Blood Groups 159 QUESTIONS WITH COMPLETE SOLUTIONS

SBB Human Blood Groups| 159 QUESTIONS| WITH COM
u u u u u u u




PLETE SOLUTIONS u




1. How would Ulex europaeus react with O, A, A2, B, A2B, A1B RBCs? -
u u u u u u u u u u u u u




✅✅✅O>A2>B>A2B>A1>A1B
u




2. How could you distinguish serologically A1, A2 and A3 -
u u u u u u u u u




u✅✅✅Dolichos biflorus can will react with A1 and not A2. A3 yields mixed u u u u u u u u u u u u u



field reactions with anti-A and anti-A,B
u u u u u




3. What is the correct order of type of RBCs to transfuse in an AB patient when A
u u u u u u u u u u u u u u u u




B RBCs are not available? Why? - ✅✅✅A, B, O; the residual anti-
u u u u u u u u u u u u



B in A RBCs is less immunogenic than the residual anti-A in B RBCs
u u u u u u u u u u u u u




4. What type of RBCs would you transfuse to a patient with anti-G -
u u u u u u u u u u u u




✅✅✅RBCs transfused must lack the D and C antigens
u u u u u u u u u




5. Can you detect a weak D using Gel Technology? -
u u u u u u u u u




✅✅✅No, because there is no washing phase
u u u u u u u




6. Can an Rh positive father and Rh positive mother yield an Rh negative child -
u u u u u u u u u u u u u u




✅✅✅Yes, if both parents are heterozygous for the D gene. Inheritance is
u u u u u u u u u u u u u



autosomal recessive u




7. What type of RBCs in regards to Rh phenotype would you transfuse in a patien
u u u u u u u u u u u u u u




t with anti-f - ✅✅✅RBCs should be negative for the c and e antigens
u u u u u u u u u u u u u




[Date] 1

,8. How could you solve an ABO discrepancy where Anti-
u u u u u u u u



M was causing aberrant results in reverse grouping when the forward type is c
u u u u u u u u u u u u u



onsistent with A blood group and reverse as O? -
u u u u u u u u u




✅✅✅Treat A1 cells with ficin to denature the M antigen, repeat reverse g
u u u u u u u u u u u u u



rouping with ficin-treated A1 cells.
u u u u




9. Why do you see an anti-N like specificity in dialysis patients? -
u u u u u u u u u u u




✅✅✅Formaldehyde induced antibody to 'N' due to formaldehyde used i
u u u u u u u u u u



n dialysis procedures.
u u




10.A patient types as follows: anti-A=4+, Anti-
u u u u u u



B=1+, A1 Cells=2+, B cells=4+. The ABO discrepancy led to suspicion of a possi
u u u u u u u u u u u u u



ble cold autoantibody based on patient histopry and diagnosis. RESt was used
u u u u u u u u u u u u



in an attept to adsorb the autoantobidy. The type was repeated with adsorbed
u u u u u u u u u u u u



uplasma Anit-A=4+, Anti- u u



B=0, A1 cells=0, B cells=0. What could be the cause of this discrepancy? -
u u u u u u u u u u u u u




u✅✅✅Anti-B was adsorbed out with RESt. u u u u u




11.What enzyme may be useful in distinguishing anti-Ge2? - ✅✅✅papain
u u u u u u u u u




12.The beta 1-3 linkage between galactose and N-
u u u u u u u




acetylglucosamine is found in? - ✅✅✅secretions u u u u u




13.Represents Type II chain - ✅✅✅Beta 1- u u u u u u



4 linkage between galactose and N acetyl glucosamine
u u u u u u u




14.Represents decreasing concentrations of H antigen on RBCs -
u u u u u u u u




✅✅✅O>A2>B>A2B>A1>A1B
u




[Date] 2

, 15.The ABO genes are located on what chromosome - ✅✅✅Chromosome 9
u u u u u u u u u u




16.Which glycosyltransferase is needed before A and B antigens are formed? -
u u u u u u u u u u u




✅✅✅L-fucosyltransferase
u




17.Which reflects the A3 subgroup - ✅✅✅mixed field reactions with Anti-
u u u u u u u u u u



A and Anti-A,B.
u u




18.Which fo the following reflects the A el subgroup? -
u u u u u u u u u




✅✅✅Positive reactions with Anti-H
u u u u




19.What clinical condition may yield positive reactions with Anti-
u u u u u u u u



A, negative with Anti-B, negative with A1 and B cells. -
u u u u u u u u u u




✅✅✅hypogammaglobulinemia
u




20.What differentiates a Bombay anti-H from an anti-H cold agglutinin? -
u u u u u u u u u u




✅✅✅reactivity at body temperature.
u u u u




21.This antigen is associated with linear carbohydrate chains - ✅✅✅i antigen
u u u u u u u u u u




22.The following reactions are found on a 35-year old male. Anti-A=4+, Anti-
u u u u u u u u u u u



B=0, Anti- u



A,B=4+, A1 cells=3+, B cells=4+, O cells=3+. How could these results be explain
u u u u u u u u u u u u




ed? - ✅✅✅Anti-M in plasma
u u u u




[Date] 3