BIO Guelph 1090 Midterm Exam Questions
And Answers |Latest 2025 | Guaranteed Pass
Composition of nucleotide - Answer✔phosphate group, sugar (5 carbon) group and nitrogen
base
purine - Answer✔double ring bases ( adenine and guanine)
pyrimidine - Answer✔single ring bases (thymine and cytosine)
bond that connects nucleotides - Answer✔phosphodiester bond between phosphate and 5-
carbon sugar group
how to identify which base from diagram - Answer✔amino(NH2) group at top: adenine and
cytosine
number of bonds between a-t - Answer✔two hydrogen bonds
number of bonds between c-g - Answer✔three hydrogen bonds
one full revolution (turn) distance - Answer✔10 base pairs = 3.4nm
distance between a base pair - Answer✔0.34 nm
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major groove vs minor groove - Answer✔major groove is the bigger space between the DNA
and minor groove is the smaller space.
what is free at 5' end - Answer✔phosphate group
what is free at 3' end - Answer✔hydroxyl group
chromosome vs chromatin - Answer✔chromosome is condensed(what we see in mitosis)
chromatin is uncondesed (sphagetti)
composition of chromosome - Answer✔protein, RNA and DNA
composition of chromatin - Answer✔primarily of DNA and proteins(histones(basic) and non
histones(acidic)) and some RNA
How many levels of chromosome packaging are there - Answer✔three.
First Level of chromosome packaging - Answer✔- packaging DNA into nucleosomes(8 histones).
- diameter of 2nm to 11nm
nucleosome core size - Answer✔146 nucleotide pairs of DNA as 1 histone is 1.75 turn of DNA
nucleosome core - Answer✔2 of each H2a, H2b, H3 and H4
complete nucleosome - Answer✔nucleosome core(2(H2a, H2b, H3 and H4) anchored by 1
histone of H1
complete nucleosome size - Answer✔166 nucleotide pairs
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