BTN 533 Exam Questions With
Answers 100% Verified
What are the units for expression of molecular mass? - ANSWER a. Moles
Is the following definition of ISU (International System of Units): - ANSWER True
Enumerate and define 5 of the basic units which form the International System of
Measurements - ANSWER Mol- Used to measure amount of material
M- Meter for length
Kg- For mass
Kelvin- For temperature
Ampere- To measure electrical current
What equation is used for nucleic acid quantification - ANSWER b. Beer-Lambert
equation
The DNA of a cell will only be found inside of the - ANSWER A. Nucleus
Genetic material sometimes needs to leave the nucleus of the cell. This can be
accomplished by using ________ to take messages to the outside of the nucleus. -
ANSWER C. RNA
When transferring liquid using a pipette, the tip of the pipette should - ANSWER C. Never
touch the side of the test tube
A typical can of Diet Coke contains 355 ml. Express in microliters and liters - ANSWER
355,000 microliters, .355 liters
A pound of M&M's weighs 453 g. Express in mg and micrograms - ANSWER 453,000 mg,
453,000,000 micrograms
The purpose of streaking a plate is to obtain - ANSWER b. bacteria in pure culture
The organisms which grows best above 45°C are called - ANSWER C. Thermophilic
What are the four distinct phases of the bacterial growth curve, in the order in which
they occur? - ANSWER b. lag phase, exponential phase, stationary phase, and death
phase
What method was used to anesthetize the fruit flies? - ANSWER b. Putting them in the
freezer for one to two minutes
, Lag phase of bacterial growth is also known as - ANSWER a. period of initial adjustment
The central dogma of molecular biology states - ANSWER d. DNA --> RNA -->
polypeptide
The set of all genes necessary to specify an organism's complete list of characteristics
is known as the - ANSWER b. genome
If a dominant gene is present but not expressed in a population, this situation is referred
to as - ANSWER B. Lack of penetrance.
An organism is homozygous dominant for trait "Q." Which statement is correct? -
ANSWER a. The genotype is QQ; the phenotype is the expression of trait Q.
The following image represents the agarose gel electrophoresis results from a
restriction digest experiment. Lane 1 is a DNA ladder and Lane 2 is the DNA sample cut
by a single restriction enzyme. What is the MINIMUM size of the uncut DNA fragment? -
ANSWER iv. 7.5 kb
Which of the following statements is true - ANSWER a. DNA fragments move through
agarose at a rate inversely proportional to their length.
b. DNA moves toward the negative electrode.
In gel electrophoresis, how do we make the DNA migrate through the gel? - ANSWER b.
We place a positive electrode away from the wells
The rate of migration of DNA within an agarose gel in the gel electrophoresis technique
is primarily based on what factor? - ANSWER d. The negative charge of the DNA
In agarose gel electrophoresis, DNA is moved towards the - ANSWER a. cathode
After electrophoresis the gel is illuminated with a _____ lamp (usually by placing it on a
light box, while using protective gear to limit exposure to ultraviolet radiation) to view
the DNA bands. - ANSWER d. ultraviolet
What are the molecular weight markers used in DNA agarose gel electrophoresis -
ANSWER a. They are usually double-stranded DNA fragments of known size.
For the visualization of DNA bands in agarose gel after electrophoresis... - ANSWER D.
All answers are correct
In Bacterial transformation the bacteria takes up and express foreign DNA usually in the
form of a_______________? - ANSWER a. Plasmid
In order to persist and be stably maintained in the cell, a plasmid DNA molecule must
contain what? - ANSWER d. origin of replication
Which of the following statement describes plasmids? - ANSWER C. Small, circular DNA
molecules that can exist independently of chromosomes commonly found in bacteria
Answers 100% Verified
What are the units for expression of molecular mass? - ANSWER a. Moles
Is the following definition of ISU (International System of Units): - ANSWER True
Enumerate and define 5 of the basic units which form the International System of
Measurements - ANSWER Mol- Used to measure amount of material
M- Meter for length
Kg- For mass
Kelvin- For temperature
Ampere- To measure electrical current
What equation is used for nucleic acid quantification - ANSWER b. Beer-Lambert
equation
The DNA of a cell will only be found inside of the - ANSWER A. Nucleus
Genetic material sometimes needs to leave the nucleus of the cell. This can be
accomplished by using ________ to take messages to the outside of the nucleus. -
ANSWER C. RNA
When transferring liquid using a pipette, the tip of the pipette should - ANSWER C. Never
touch the side of the test tube
A typical can of Diet Coke contains 355 ml. Express in microliters and liters - ANSWER
355,000 microliters, .355 liters
A pound of M&M's weighs 453 g. Express in mg and micrograms - ANSWER 453,000 mg,
453,000,000 micrograms
The purpose of streaking a plate is to obtain - ANSWER b. bacteria in pure culture
The organisms which grows best above 45°C are called - ANSWER C. Thermophilic
What are the four distinct phases of the bacterial growth curve, in the order in which
they occur? - ANSWER b. lag phase, exponential phase, stationary phase, and death
phase
What method was used to anesthetize the fruit flies? - ANSWER b. Putting them in the
freezer for one to two minutes
, Lag phase of bacterial growth is also known as - ANSWER a. period of initial adjustment
The central dogma of molecular biology states - ANSWER d. DNA --> RNA -->
polypeptide
The set of all genes necessary to specify an organism's complete list of characteristics
is known as the - ANSWER b. genome
If a dominant gene is present but not expressed in a population, this situation is referred
to as - ANSWER B. Lack of penetrance.
An organism is homozygous dominant for trait "Q." Which statement is correct? -
ANSWER a. The genotype is QQ; the phenotype is the expression of trait Q.
The following image represents the agarose gel electrophoresis results from a
restriction digest experiment. Lane 1 is a DNA ladder and Lane 2 is the DNA sample cut
by a single restriction enzyme. What is the MINIMUM size of the uncut DNA fragment? -
ANSWER iv. 7.5 kb
Which of the following statements is true - ANSWER a. DNA fragments move through
agarose at a rate inversely proportional to their length.
b. DNA moves toward the negative electrode.
In gel electrophoresis, how do we make the DNA migrate through the gel? - ANSWER b.
We place a positive electrode away from the wells
The rate of migration of DNA within an agarose gel in the gel electrophoresis technique
is primarily based on what factor? - ANSWER d. The negative charge of the DNA
In agarose gel electrophoresis, DNA is moved towards the - ANSWER a. cathode
After electrophoresis the gel is illuminated with a _____ lamp (usually by placing it on a
light box, while using protective gear to limit exposure to ultraviolet radiation) to view
the DNA bands. - ANSWER d. ultraviolet
What are the molecular weight markers used in DNA agarose gel electrophoresis -
ANSWER a. They are usually double-stranded DNA fragments of known size.
For the visualization of DNA bands in agarose gel after electrophoresis... - ANSWER D.
All answers are correct
In Bacterial transformation the bacteria takes up and express foreign DNA usually in the
form of a_______________? - ANSWER a. Plasmid
In order to persist and be stably maintained in the cell, a plasmid DNA molecule must
contain what? - ANSWER d. origin of replication
Which of the following statement describes plasmids? - ANSWER C. Small, circular DNA
molecules that can exist independently of chromosomes commonly found in bacteria
