ACS BIOCHEMISTRY EXAM 3 LATEST EXAM ACTUAL EXAM COMPLETE REAL QUESTIONS AND CORRECT DETAILED ANSWERS (CORRECT VERIFIED SOLUTIONS) NEWEST UPDATED VERSION

ACS BIOCHEMISTRY
EXAM Questions
and Answers
h




Henderson-


HasselbachhEquationhAns-


pHh=hpKah+hlogh([A-]h/h[HA])




FMOChChemicalhSynthesis


Ans-

Usedhinhsynthesishofhahgrowinghaminohacidhchainhtohahpolystyrenehbead.hF

MOChishusedhashahprotectinghgrouphonhthehN-terminus.




SaltinghOuth(Purification)


Ans-

Changeshsolublehproteinhtohsolidhprecipitate.hProteinhprecipitateshwhenh

thehchargeshonhthehproteinhmatchhthehchargeshinhthehsolution.

,Size-ExclusionhChromatography


Ans- Separateshsamplehbasedhonhsizehwithhsmallerhmoleculeshelutinghlater.




Ion-ExchangehChromatography


Ans- Separateshsamplehbasedhonhcharge.hCMhattractsh+,hDEAEhattractsh-

.hMayhhavehrepulsionheffecthonhlikehcharges.hSalthorhacidhusedhtohremovehstuckhpr

oteins.




Hydrophobic/ReversehPhasehChromatography


Ans-

Beadsharehcoatedhwithhahcarbonhchain.hHydrophobichproteinshstickhbetter.h

Elutehwithhnon-H-bondinghsolventh(acetonitrile).




AffinityhChromatography


Ans-

Attachhahligandhthathbindshahproteinhtohahbead.hElutehwithhharshhchemicalsho

rhsimilarhligand.




SDS-PAGE

,Ans- UseshSDS.hGelhishmadehfromhcross-

linkedhpolyacrylamide.hSeparateshbasedhoffhofhmasshwithhsmallerhmoleculesh

movinghfaster.hVisualizedhwithhCoomassiehblue.




SDS


Ans-

Sodiumhdodecylhsulfate.hUnfoldshproteinshandhgiveshthemhuniformhnegativeh

charge.




IsoelectrichFocusing


Ans-

Variationhofhgelhelectrophoresishwherehproteinhchargehmatters.hInvolvesh

electrodeshandhpHhgradient.hProteinhstopshaththeirhpIhwhenhneutral.




FDNBh(1-fluoro-2,3-dinitrobenzene)


Ans- FDNBhreactshwithhthehN-terminushofhthehproteinhtohproducehah2,4-

dinitrophenolhderivativehthathlabelshthehfirsthresidue.hCanhrepeathhydrolysishtoh
h




determinehsequentialhaminohacids.




DTTh(dithiothreitol)

, Ans- Reduceshdisulfidehbonds.




Iodoacetate


Ans- Addshcarboxymethylhgrouphonhfreeh-SHhgroups.hBlockshdisulfidehbonding.




Homologs


Ans- Sharesh25%hidentityhwithhanotherhgene




Orthologs


Ans- Similarhgeneshinhdifferenthorganisms




Paralogs


Ans- Similarh"paired"hgeneshinhthehsamehorganism




RamachandranhPlot


Ans- Showshfavorablehphi-psihanglehcombinations.h3hmainh"wells"hforhα-

helices,hß-sheets,handhleft-handedhα-helices.