Page | 1h h
ACS BIOCHEMISTRY EXAM 2025| BRAND N
h h h h h
EW ACTUAL EXAM WITH 100% VERIFIED Q
h h h h h h
UESTIONS AND CORRECT SOLUTIONS| GU h h h h
ARANTEED VALUE PACK| ACE YOUR GRAD h h h h h
ES.
Step 1 of Glycolysis - correct answer - Glucose --> Glucose 6-
h h h h h h h h h h h
phosphate. Uses hexokinase enzyme. h h h
ATP --> ADP h h
Step 2 of Glycolysis - correct answer - Glucose 6-phosphate <--
h h h h h h h h h h
> Fructose 6-phosphate
h h
Uses phosphohexose isomerase enzyme.
h h h
Step 3 of Glycolysis - correct answer - Fructose 6-phosphate --
h h h h h h h h h h
> Fructose 1,6-bisphosphate
h h
Uses PFK-1 (phosphofructokinase-
h h
1) enzyme. ATP --> ADP
h h h h
First Committed Step of Glycolysis - correct answer -
h h h h h h h h
Step 3 of Glycolysis. Fructose 6-Phosphate --> Fructose 1,6-
h h h h h h h h h
bisphosphate. (PFK-1) h
, Page | 2 h h
Step 4 of Glycolysis - correct answer - Fructose 1,6-bisphosphate <--
h h h h h h h h h h
> dihydroxyacetone + glyceraldehyde 3-phosphate.
h h h h
Uses aldolase enzyme.
h h
Step 5 of Glycolysis - correct answer - Dihydroxyacetonephosphate <--
h h h h h h h h h
> glyceraldehyde 3-phosphate
h h
Uses triose phosphate isomerase enzyme.
h h h h
Step 6 of Glycolysis - correct answer - Glyceraldehyde 3-Phosphate + Pi <-
h h h h h h h h h h h h
-> 1,3-biphosphoglycerate.
h
Uses G3P dehydrogenase enzyme. NA
h h h h
D+ <--> NADHh h
First Energy Yielding Step of Glycolysis - correct answer -
h h h h h h h h h
Step 6 of Glycolysis.
h h h h
G3P + Pi <--> 1,3-bisphosphoglycerate
h h h h
Step 7 of Glycolysis - correct answer - 1,3-bisphosphoglycerate + ADP <--
h h h h h h h h h h h
> 3-phosphoglycerate + ATP
h h h
Uses phosphoglycerate kinase enzyme.
h h h
, Page | 3
h h
First ATP Yielding Step of Glycolysis - correct answer - Step 7 of Glycolysis.
h h h h h h h h h h h h h
1,3-bisphosphoglycerate <--> 3-phosphoglycerate h h
Step 8 of Glycolysis - correct answer - 3-phosphoglycerate <--> 2-
h h h h h h h h h h
phosphoglycerate
h
Uses phosphoglycerate mutase enzyme.
h h h
Step 9 of Glycolysis - correct answer - 2-phosphoglycerate <--
h h h h h h h h h
> Phosphoenolpyruvate (PEP)
h h
Uses enolase enzyme.
h h
Dehydration reaction (loss of water). h h h h
Step 10 of Glycolysis - correct answer - PEP + ADP --
h h h h h h h h h h h
> Pyruvate + ATP Uses pyruvate kinase enzyme.
h h h h h h h
ATP Consuming Steps of Glycolysis - correct answer -
h h h h h h h h
Step 1 and 3. Glucose --> Glucose 6-phosphate
h h h h h h h h
Fructose 6-phosphate --> Fructose 1,6-bisphosphate
h h h h
, Page | 4 h h
ATP Producing Steps of Glycolysis - correct answer -
h h h h h h h h
Steps 7 and 10. 1,3-bisphosphoglycerate <--> 3-phosphoglycerate
h h h h h h h
PEP --> Pyruvate
h h
NADH Producing Step of Glycolysis - correct answer -
h h h h h h h h
Step 6 G3P <--> 1,3-bisphosphoglycerate
h h h h h
Total Energy Produced by Glycolysis - correct answer - 2NADH + 4 ATP
h h h h h h h h h h h h
Lactic Acid Fermentation - correct answer - Pyruvate --> L-
h h h h h h h h h
Lactate NADH --> NAD+ h h h
Regenerates NAD+ for use in glycolysis h h h h h
Ethanol Fermentation - correct answer - Pyruvate --> Acetalaldehyde --
h h h h h h h h h
> Ethanol
h
Uses pyruvate decarboxylase (TPP) and alcohol dehydrogenase. NAD
h h h h h h h
H --> CO2(TPP) + NAD+
h h h h
TPP Cofactor Structure - correct answer -
h h h h h h
ACS BIOCHEMISTRY EXAM 2025| BRAND N
h h h h h
EW ACTUAL EXAM WITH 100% VERIFIED Q
h h h h h h
UESTIONS AND CORRECT SOLUTIONS| GU h h h h
ARANTEED VALUE PACK| ACE YOUR GRAD h h h h h
ES.
Step 1 of Glycolysis - correct answer - Glucose --> Glucose 6-
h h h h h h h h h h h
phosphate. Uses hexokinase enzyme. h h h
ATP --> ADP h h
Step 2 of Glycolysis - correct answer - Glucose 6-phosphate <--
h h h h h h h h h h
> Fructose 6-phosphate
h h
Uses phosphohexose isomerase enzyme.
h h h
Step 3 of Glycolysis - correct answer - Fructose 6-phosphate --
h h h h h h h h h h
> Fructose 1,6-bisphosphate
h h
Uses PFK-1 (phosphofructokinase-
h h
1) enzyme. ATP --> ADP
h h h h
First Committed Step of Glycolysis - correct answer -
h h h h h h h h
Step 3 of Glycolysis. Fructose 6-Phosphate --> Fructose 1,6-
h h h h h h h h h
bisphosphate. (PFK-1) h
, Page | 2 h h
Step 4 of Glycolysis - correct answer - Fructose 1,6-bisphosphate <--
h h h h h h h h h h
> dihydroxyacetone + glyceraldehyde 3-phosphate.
h h h h
Uses aldolase enzyme.
h h
Step 5 of Glycolysis - correct answer - Dihydroxyacetonephosphate <--
h h h h h h h h h
> glyceraldehyde 3-phosphate
h h
Uses triose phosphate isomerase enzyme.
h h h h
Step 6 of Glycolysis - correct answer - Glyceraldehyde 3-Phosphate + Pi <-
h h h h h h h h h h h h
-> 1,3-biphosphoglycerate.
h
Uses G3P dehydrogenase enzyme. NA
h h h h
D+ <--> NADHh h
First Energy Yielding Step of Glycolysis - correct answer -
h h h h h h h h h
Step 6 of Glycolysis.
h h h h
G3P + Pi <--> 1,3-bisphosphoglycerate
h h h h
Step 7 of Glycolysis - correct answer - 1,3-bisphosphoglycerate + ADP <--
h h h h h h h h h h h
> 3-phosphoglycerate + ATP
h h h
Uses phosphoglycerate kinase enzyme.
h h h
, Page | 3
h h
First ATP Yielding Step of Glycolysis - correct answer - Step 7 of Glycolysis.
h h h h h h h h h h h h h
1,3-bisphosphoglycerate <--> 3-phosphoglycerate h h
Step 8 of Glycolysis - correct answer - 3-phosphoglycerate <--> 2-
h h h h h h h h h h
phosphoglycerate
h
Uses phosphoglycerate mutase enzyme.
h h h
Step 9 of Glycolysis - correct answer - 2-phosphoglycerate <--
h h h h h h h h h
> Phosphoenolpyruvate (PEP)
h h
Uses enolase enzyme.
h h
Dehydration reaction (loss of water). h h h h
Step 10 of Glycolysis - correct answer - PEP + ADP --
h h h h h h h h h h h
> Pyruvate + ATP Uses pyruvate kinase enzyme.
h h h h h h h
ATP Consuming Steps of Glycolysis - correct answer -
h h h h h h h h
Step 1 and 3. Glucose --> Glucose 6-phosphate
h h h h h h h h
Fructose 6-phosphate --> Fructose 1,6-bisphosphate
h h h h
, Page | 4 h h
ATP Producing Steps of Glycolysis - correct answer -
h h h h h h h h
Steps 7 and 10. 1,3-bisphosphoglycerate <--> 3-phosphoglycerate
h h h h h h h
PEP --> Pyruvate
h h
NADH Producing Step of Glycolysis - correct answer -
h h h h h h h h
Step 6 G3P <--> 1,3-bisphosphoglycerate
h h h h h
Total Energy Produced by Glycolysis - correct answer - 2NADH + 4 ATP
h h h h h h h h h h h h
Lactic Acid Fermentation - correct answer - Pyruvate --> L-
h h h h h h h h h
Lactate NADH --> NAD+ h h h
Regenerates NAD+ for use in glycolysis h h h h h
Ethanol Fermentation - correct answer - Pyruvate --> Acetalaldehyde --
h h h h h h h h h
> Ethanol
h
Uses pyruvate decarboxylase (TPP) and alcohol dehydrogenase. NAD
h h h h h h h
H --> CO2(TPP) + NAD+
h h h h
TPP Cofactor Structure - correct answer -
h h h h h h
