4/27/25, 11:11
AM
BIO 229 FINAL EXAM QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED GRADED A++
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Practice questions for this set
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blocking reagent keeps AB from binding to surface, the primary antibody binds to a
specific antigen, another antibody is added to bind to the secondary AB and contains an enzyme,
substrate comes in and binds so it can be detected
Select the correct term
1is peptidases intracellular or extracellular? 2what does non capture Elisa test for
3virulence
4how does indirect Elisa work?
Don't know?
Terms in this set (142)
How to determine indirect detection of by-proudcts
is the enzyme secreted from cell, usually a pH
1/
10
, 4/27/25, 11:11
AM
activity is indicator or or cell lysis followed by
intracellular? above
Peptidases A group of enzymes that break down
(Proteases) proteins, too large to transport across
membrane
is peptidases extracellular digestion - peptidases secreted to
intracellular or hydrolyze proteins to
extracellular? manageable size for transport (exotoxins)
casein proteases casein - milk protein, 80% of total in cow's milk,
agar white color
how to determine if if positive it results in a clearing (halo) and if
the casein negative then no change in agar
protease test is
positive or
negative
what does a halo casein hydrolysis
indicate for casein
agar
pathogenicity ability of pathogen to cause disease
virulence relative ability of pathogen to cause a disease
virulence factors product/structure of pathogens that
enable invasivness by promoting
colonization and growth
toxicity ability to cause disease by means of toxins that
inhibit function or cause cell death
what is coagulase clots fibrinogen in plasma
mechanism of action
what is collagenase degrades collagen
mechanism of action
what is DNAse lowers viscosity of exudates increasing
mechanism of action pathogen motility
what is hemolysin lyse erythrocytes and other cell types, cause
mechanism of action anemia
collagen primary component of connective tissue and
extracellular
what degrades collagenases and produces gelatin
collagen
what degrades gelatins and product petites and amino aicids
gelatin
what is the gelatins a nutrient gelatin tube with peptones and beef
2/
10
AM
BIO 229 FINAL EXAM QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED GRADED A++
Leave the first rating
Save
Practice questions for this set
Learn 1/7 Study using Learn
blocking reagent keeps AB from binding to surface, the primary antibody binds to a
specific antigen, another antibody is added to bind to the secondary AB and contains an enzyme,
substrate comes in and binds so it can be detected
Select the correct term
1is peptidases intracellular or extracellular? 2what does non capture Elisa test for
3virulence
4how does indirect Elisa work?
Don't know?
Terms in this set (142)
How to determine indirect detection of by-proudcts
is the enzyme secreted from cell, usually a pH
1/
10
, 4/27/25, 11:11
AM
activity is indicator or or cell lysis followed by
intracellular? above
Peptidases A group of enzymes that break down
(Proteases) proteins, too large to transport across
membrane
is peptidases extracellular digestion - peptidases secreted to
intracellular or hydrolyze proteins to
extracellular? manageable size for transport (exotoxins)
casein proteases casein - milk protein, 80% of total in cow's milk,
agar white color
how to determine if if positive it results in a clearing (halo) and if
the casein negative then no change in agar
protease test is
positive or
negative
what does a halo casein hydrolysis
indicate for casein
agar
pathogenicity ability of pathogen to cause disease
virulence relative ability of pathogen to cause a disease
virulence factors product/structure of pathogens that
enable invasivness by promoting
colonization and growth
toxicity ability to cause disease by means of toxins that
inhibit function or cause cell death
what is coagulase clots fibrinogen in plasma
mechanism of action
what is collagenase degrades collagen
mechanism of action
what is DNAse lowers viscosity of exudates increasing
mechanism of action pathogen motility
what is hemolysin lyse erythrocytes and other cell types, cause
mechanism of action anemia
collagen primary component of connective tissue and
extracellular
what degrades collagenases and produces gelatin
collagen
what degrades gelatins and product petites and amino aicids
gelatin
what is the gelatins a nutrient gelatin tube with peptones and beef
2/
10
