AM
MICRO 3050 Exam 4 QUESTIONS AND ANSWERS WITH COMPLETE
SOLUTIONS VERIFIED GRADED A++
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The intestinal d. all of the above
microbiota (microflora)
plays a role in:
a.Training the immune
system
b. Production of
essential vitamins for
the host
c. Colonization
resistance
d. All of the above
Which one of the c. They cause normal viral infections resulting
following statements in the release of newly synthesized virions
does NOT describe upon lysis of the host
transducing
particles?
a. They play a role in
the transfer of
bacterial genes
b. They are defective
bacteriophages
c. They cause normal
viral infections
resulting in the
release of newly
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synthesized virions upon
lysis of the host
d. They result from
a mistake in the
packaging of DNA
into phage heads
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Suppose you are a. Cut the plasmid and the DNA containing
making a recombinant the gene of interest with the same
plasmid. Which one restriction enzyme to generate sticky ends.
of the following Glue the gene of interest into the plasmid
protocols would work using DNA ligase
the best?
Cut the plasmid and
a.
the DNA
containing the gene
of interest with the
same restriction
enzyme to generate
sticky ends. Glue the
gene of interest into
the plasmid using
DNA ligase
b. Cut the plasmid and
the DNA
containing the gene of
interest with
different primers to
generate blunt ends.
Glue the gene of
interest into the
plasmid using a
thermocycler
c. Cut the plasmid and
the DNA
containing the gene of
interest with the same
restriction enzyme to
generate
blunt ends. Glue the
gene of interest into
the plasmid using
Taq polymerase
d. Cut the plasmid and
the DNA
containing the gene
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