,Biosc 1000 Spring 2025 2/4/2025
Lesson 7 - Ligand Binding and Motor Proteins
Learning Objectives
You should be able to:
• Give characteristics of the five major functional classes of proteins
• Understand the molecular basis for specificity and reversibility of protein-ligand binding.
• Define KD and θ as they relate to binding proteins.
• Describe protein-ligand interactions mathematically and graphically.
• Explain how myosin structure relates to its function as a motor protein.
• Define the composition of thick and thin filaments in myofibrils.
• Describe the molecular basis for the sliding filament model of muscle contraction.
Textbook Reference
Text Chapter 6, Section 1, Section 2 (pp. 275-277) and Section 4
Vocabulary
• ligand
• KD
• binding curve
• globular protein
• prosthetic group
• conformation
• actin
• myosin
Lecture Slides (begin next page)
This material is for the use of Biosc 1000 students only and cannot be distributed electronically, in print, or
posted on any electronic forum. Doing so is a breach of academic integrity and will be prosecuted as such.
©2025 Laura S. Zapanta, Ph.D. 1
,Pre-Class Renew
Ligand binding ea"
P +L = PL
amsto
Dissoc constant (kc) =
↑ had So % sat = [L] (0 0 5) =
.
0 S.
= 0
: EPL]
-
Kd =
kd =
L
Matt S
3
Kd = 1 .
Sun (1 SmM Zumm) .
:
Plot =
10mM = PL +P amyPL =
zSmm2
2 SmM PL 6:25 wm -62 S %
freelig
: :
.
.
O - T
practice
More Prac
↑
2 Sma
.
=
25 %
-
(PL]
PL + P 7 Sum
-
Kiss
= .
-
recepter
=
conc = IOm
25 - 1.
occupied s
free ISMM
liger
:
Binding corres-single ligand
to
single protein
Kd G &O S Chalf sat
s
= .
in X-axis measured units
oe
f ss
gasees is in
, Biosc 1000 Spring 2025 2/4/2025
Motor Proteins
Lesson 7.2
Biosc 1000 – Lecture 7
February 4, 2025
7
7
Molecular motors are proteins that use ATP to produce
cyclic conformational changes.
garerend by ligands
12 bun
bind
release
8
8
©2025 Laura S. Zapanta, Ph.D. 2
Lesson 7 - Ligand Binding and Motor Proteins
Learning Objectives
You should be able to:
• Give characteristics of the five major functional classes of proteins
• Understand the molecular basis for specificity and reversibility of protein-ligand binding.
• Define KD and θ as they relate to binding proteins.
• Describe protein-ligand interactions mathematically and graphically.
• Explain how myosin structure relates to its function as a motor protein.
• Define the composition of thick and thin filaments in myofibrils.
• Describe the molecular basis for the sliding filament model of muscle contraction.
Textbook Reference
Text Chapter 6, Section 1, Section 2 (pp. 275-277) and Section 4
Vocabulary
• ligand
• KD
• binding curve
• globular protein
• prosthetic group
• conformation
• actin
• myosin
Lecture Slides (begin next page)
This material is for the use of Biosc 1000 students only and cannot be distributed electronically, in print, or
posted on any electronic forum. Doing so is a breach of academic integrity and will be prosecuted as such.
©2025 Laura S. Zapanta, Ph.D. 1
,Pre-Class Renew
Ligand binding ea"
P +L = PL
amsto
Dissoc constant (kc) =
↑ had So % sat = [L] (0 0 5) =
.
0 S.
= 0
: EPL]
-
Kd =
kd =
L
Matt S
3
Kd = 1 .
Sun (1 SmM Zumm) .
:
Plot =
10mM = PL +P amyPL =
zSmm2
2 SmM PL 6:25 wm -62 S %
freelig
: :
.
.
O - T
practice
More Prac
↑
2 Sma
.
=
25 %
-
(PL]
PL + P 7 Sum
-
Kiss
= .
-
recepter
=
conc = IOm
25 - 1.
occupied s
free ISMM
liger
:
Binding corres-single ligand
to
single protein
Kd G &O S Chalf sat
s
= .
in X-axis measured units
oe
f ss
gasees is in
, Biosc 1000 Spring 2025 2/4/2025
Motor Proteins
Lesson 7.2
Biosc 1000 – Lecture 7
February 4, 2025
7
7
Molecular motors are proteins that use ATP to produce
cyclic conformational changes.
garerend by ligands
12 bun
bind
release
8
8
©2025 Laura S. Zapanta, Ph.D. 2
