A+ questions with correct verified detailed
answers latest update 2025
Lysogeny Broth (LB) --- correct answers ---standard media; defined
Lysogeny broth components --- correct answers ---peptides, vitamins,
minerals, water
Luria Broth --- correct answers ---0.5g/L; mimics biological condition
of body
agar-agar --- correct answers ---red algae; porous, non-digestible,
complex sugar
bacterial cultivation --- correct answers ---1.5% agar by weight
Robert Koch --- correct answers ---isolated and identified tuberculosis
Angelina Hesse --- correct answers ---agar
, agar --- correct answers ---resilience, stability in heat, lack of
toxicity, ages well
Preparing a Bacterial Smear --- correct answers ---1. water on slide
2.transfer bacteria w/ loop
3. mix bacteria/water, spread
4. air dry
5. put slide through Bunsen burner 3X
HFBS --- correct answers ---heat fixing bacterial smear; less is more;
entirely dry; don't overheat
gram stain --- correct answers ---bacterias ability to retain dye;
mostly pathogenic
differential stain --- correct answers ---two or more dyes
Gram stain procedure --- correct answers ---1. heat fix slide
2. crystal violet (30 sec)
3. iodine (30 sec)