DNA replication Enzy mes a Steps (prokaryotes
*
Histone protein :
Eukaryotes Prokaryotes
·
occur before cell division VInucleosomel X(naked DNA)
1. Helicase unwind supercoiled DNA >
-
Amount of DNA >
- check point
> cell grow size
-
separate 2 strands replication fork +
G2 > GI
single strand binding protein keep strands
metabolic
> normal
-
·
:
↳ cytoplasm eg .
protein synthesis
> cell grow
from together
-
>
-
prepare mitosis >
-
Mitochondria a
eg synthes is
chloroplast undergo
.
microtubules
fission
Gyrase
binary
nucleus
-
cytoplasm
.
2 : before helicase relieve ,
tension of
>
-
>
- DNA replication
-
for
>
- check point
ready for DNA
synthesis
supercoiling
DNA primase
cell division
3
. : attach RNA primers to
template strand , as a
starting point
·
leading strand 1
:
Semi-conservative nature double strand
lagging strand
many -
·
:
-
each new
has one
original a one
newly synthesized strands 4
. DNA polymerase 'I along 5'-3' (new strand
↓
- move
strand
each
original act as template
·
free
adding nucleotides
3 theories :
5 DNA polymerase I remove
primers & replace w/
high accuracy
.
& NA nucleotides
& 6
. DNA
ligase catalyzes phosphodiester bonds
# between Okazaki fragments .
Meselson a Stahl experiment (*N)
using "heavy"
: &
"light" (*N) isotopes .
*
Histone protein :
Eukaryotes Prokaryotes
·
occur before cell division VInucleosomel X(naked DNA)
1. Helicase unwind supercoiled DNA >
-
Amount of DNA >
- check point
> cell grow size
-
separate 2 strands replication fork +
G2 > GI
single strand binding protein keep strands
metabolic
> normal
-
·
:
↳ cytoplasm eg .
protein synthesis
> cell grow
from together
-
>
-
prepare mitosis >
-
Mitochondria a
eg synthes is
chloroplast undergo
.
microtubules
fission
Gyrase
binary
nucleus
-
cytoplasm
.
2 : before helicase relieve ,
tension of
>
-
>
- DNA replication
-
for
>
- check point
ready for DNA
synthesis
supercoiling
DNA primase
cell division
3
. : attach RNA primers to
template strand , as a
starting point
·
leading strand 1
:
Semi-conservative nature double strand
lagging strand
many -
·
:
-
each new
has one
original a one
newly synthesized strands 4
. DNA polymerase 'I along 5'-3' (new strand
↓
- move
strand
each
original act as template
·
free
adding nucleotides
3 theories :
5 DNA polymerase I remove
primers & replace w/
high accuracy
.
& NA nucleotides
& 6
. DNA
ligase catalyzes phosphodiester bonds
# between Okazaki fragments .
Meselson a Stahl experiment (*N)
using "heavy"
: &
"light" (*N) isotopes .
