GUARANTEED PASS
Which of the following statement describes plasmids? C. Small, circular DNA molecules that
can exist independently of chromosomes commonly found in bacteria
The purpose of calcium chloride is to allow the plasmid to pass through the bacterial cell wall?
True
In transformation, both strands of donor DNA enter the recipient cell. False
The plasmids can be eliminated from a cell by the process known as d. expulsion
DNA transformation involves the transfer of naked DNA into a recipient cell. True
Transformation means: b. acquiring DNA from the bacterial cell environment
In nature, the purpose of restriction enzymes is to: D. Protect the bacterium from virus
attack by cutting up foreign DNA
When "sticky ends" are paired, they can be joined by E) DNA ligase
All restriction enzyme recognition sites d. Are read the same forward and backward.
35. Bacterial DNA is not cleaved by their own restriction enzymes because bacteria add
_______________ to their own DNA. C. Methyl groups
, To clone into a plasmid vector, both the plasmid and the foreign DNA are cut: a. with the
same restriction enzyme and mixed together
Which of the following scientific breakthroughs did NOT develop from DNA cloning? c. The
creation of herbicide resistant tobacco plants in 1986
35. One end of a polynucleotide has a (n) _____ terminus, and the other end has a (n) _____
terminus. (1 point) c. 5′-P; 3′-OH
The term "blotting" refers to c. Transfer of biomolecules to an immobilizing membrane
Proteins that are to be separated in SDS-PAGE are subjected to treat with all of the following
reagents except: e. Cold ice cream
Which tracking dye did you use in SDS-PAGE? a. Coomassie blue
In order to denature proteins by binding to the polypeptide backbone; SDS should have which
concentration on the amino acid chain? b. 1.4 g / 1 g protein
Organisms which have a distinct nucleus with chromatin material and a nucleolus are called
b. eukaryotic
The cytoplasm is filled with 80% of b. water
Which of the following is correct? d. All of the above
Bacteriophages are c. bacterial viruses