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BTN 533| QUESTIONS WITH VERIFIED ANSWERS 100% SOLVED| LATEST UPDATE GUARANTEED PASS

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BTN 533| QUESTIONS WITH VERIFIED ANSWERS 100% SOLVED| LATEST UPDATE GUARANTEED PASS

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BTN 533
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BTN 533| QUESTIONS WITH VERIFIED ANSWERS 100% SOLVED| LATEST UPDATE
GUARANTEED PASS



Which of the following statement describes plasmids? C. Small, circular DNA molecules that
can exist independently of chromosomes commonly found in bacteria



The purpose of calcium chloride is to allow the plasmid to pass through the bacterial cell wall?
True



In transformation, both strands of donor DNA enter the recipient cell. False



The plasmids can be eliminated from a cell by the process known as d. expulsion



DNA transformation involves the transfer of naked DNA into a recipient cell. True



Transformation means: b. acquiring DNA from the bacterial cell environment



In nature, the purpose of restriction enzymes is to: D. Protect the bacterium from virus
attack by cutting up foreign DNA



When "sticky ends" are paired, they can be joined by E) DNA ligase



All restriction enzyme recognition sites d. Are read the same forward and backward.



35. Bacterial DNA is not cleaved by their own restriction enzymes because bacteria add
_______________ to their own DNA. C. Methyl groups

, To clone into a plasmid vector, both the plasmid and the foreign DNA are cut: a. with the
same restriction enzyme and mixed together



Which of the following scientific breakthroughs did NOT develop from DNA cloning? c. The
creation of herbicide resistant tobacco plants in 1986



35. One end of a polynucleotide has a (n) _____ terminus, and the other end has a (n) _____
terminus. (1 point) c. 5′-P; 3′-OH



The term "blotting" refers to c. Transfer of biomolecules to an immobilizing membrane



Proteins that are to be separated in SDS-PAGE are subjected to treat with all of the following
reagents except: e. Cold ice cream



Which tracking dye did you use in SDS-PAGE? a. Coomassie blue



In order to denature proteins by binding to the polypeptide backbone; SDS should have which
concentration on the amino acid chain? b. 1.4 g / 1 g protein



Organisms which have a distinct nucleus with chromatin material and a nucleolus are called
b. eukaryotic



The cytoplasm is filled with 80% of b. water



Which of the following is correct? d. All of the above



Bacteriophages are c. bacterial viruses

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