BTN 533 FINAL EXAM | QUESTIONS WITH VERIFIED ANSWERS 100% SOLVED| LATEST UPDATE
GUARANTEED PASS
To clone into a plasmid vector, both the plasmid and the foreign DNA are cut
A. with the same restriction enzyme and mixed together
B. with different restriction enzyme and mixed together
C. with the combination of enzymes and then separated
D. with the combination of enzymes and mixed together. A. with the same restriction
enzyme and mixed together
PCR can be used to amplify a specific fragment of DNA from which of the following?
A. A drop of blood
B. A hair follicle
C. A fragment of skin
D. All of these. D. All of these.
Which of the following is correct?
A. HIV is a retrovirus that kills human helper T cells
B. Causes acquired immune deficiency syndrome (AIDS)
C. Cripples the immunity systems
D. All of the above. D. All of the above.
PCR can be used in
,A. cloning
B. sequencing
C. medical diagnosis and forensic medicine
D. all of the above. D. all of the above.
PCR (Polymerase Chain Reaction) can make multiple copies of
A. DNA
B. RNA
C. protein
D. carbohydrate. A. DNA
PCR amplifies millions of copies of
A. a short fragment of DNA
B. the entire genome (all the DNA in a sample)
C. both (a) and (b)
D. short chain RNA. A. a short fragment of DNA
The PCR, polymerase chain reaction is becoming the method of choice for
A. Alteration of gene
B. screening gene
C. sterilization of gene
D. all of these B. screening gene
,DNA fragments in a restriction digest can be separated by electrophoresis in
A. polyacrylamide
B. agarose gel
C. both (a) and (b)
D. none of these. C. both (a) and (b)
Which of the following hybridize with the ends of the gene to be amplified?
A) Taq polymerase
B) deoxyribonucleotides
C) ribonucleotides
D) DNA molecules
E) DNA primers. E) DNA primers.
This new complementary strand of DNA is constructed of which of the following?
A) Taq polymerase
B) deoxyribonucleotides
C) ribonucleotides
D) DNA molecules
E) DNA primers. A) Taq polymerase
By running the polymerase chain reaction overnight for twenty-one cycles, a single segment of
DNA can be amplified to around a thousand copies.
, A) True B) False B) False
The polymerase chain reaction is a method for making copies of small amounts of several
segments of DNA.
A) True B) False B) False
The purpose of PCR is to
A) make more copies of DNA primers to increase protein synthesis
B) make many copies of an organism's DNA sequence so a small number of
organisms will become large enough to be identified
C) make more RNA so large units of protein can be synthesized
D) recycle DNA using thermocyclers B) make many copies of an organism's DNA sequence so
a small number of
organisms will become large enough to be identified
Taq polymerase starts copying at
A) the end of free single-stranded RNA
B) any open point
C) RNA primers attached to the end of the desired gene
D) DNA primers attached to the end of the desired gene. D) DNA primers attached to the
end of the desired gene.
Using PCR, over 1 million copies of DNA can be made from a single strand of DNA overnight.
GUARANTEED PASS
To clone into a plasmid vector, both the plasmid and the foreign DNA are cut
A. with the same restriction enzyme and mixed together
B. with different restriction enzyme and mixed together
C. with the combination of enzymes and then separated
D. with the combination of enzymes and mixed together. A. with the same restriction
enzyme and mixed together
PCR can be used to amplify a specific fragment of DNA from which of the following?
A. A drop of blood
B. A hair follicle
C. A fragment of skin
D. All of these. D. All of these.
Which of the following is correct?
A. HIV is a retrovirus that kills human helper T cells
B. Causes acquired immune deficiency syndrome (AIDS)
C. Cripples the immunity systems
D. All of the above. D. All of the above.
PCR can be used in
,A. cloning
B. sequencing
C. medical diagnosis and forensic medicine
D. all of the above. D. all of the above.
PCR (Polymerase Chain Reaction) can make multiple copies of
A. DNA
B. RNA
C. protein
D. carbohydrate. A. DNA
PCR amplifies millions of copies of
A. a short fragment of DNA
B. the entire genome (all the DNA in a sample)
C. both (a) and (b)
D. short chain RNA. A. a short fragment of DNA
The PCR, polymerase chain reaction is becoming the method of choice for
A. Alteration of gene
B. screening gene
C. sterilization of gene
D. all of these B. screening gene
,DNA fragments in a restriction digest can be separated by electrophoresis in
A. polyacrylamide
B. agarose gel
C. both (a) and (b)
D. none of these. C. both (a) and (b)
Which of the following hybridize with the ends of the gene to be amplified?
A) Taq polymerase
B) deoxyribonucleotides
C) ribonucleotides
D) DNA molecules
E) DNA primers. E) DNA primers.
This new complementary strand of DNA is constructed of which of the following?
A) Taq polymerase
B) deoxyribonucleotides
C) ribonucleotides
D) DNA molecules
E) DNA primers. A) Taq polymerase
By running the polymerase chain reaction overnight for twenty-one cycles, a single segment of
DNA can be amplified to around a thousand copies.
, A) True B) False B) False
The polymerase chain reaction is a method for making copies of small amounts of several
segments of DNA.
A) True B) False B) False
The purpose of PCR is to
A) make more copies of DNA primers to increase protein synthesis
B) make many copies of an organism's DNA sequence so a small number of
organisms will become large enough to be identified
C) make more RNA so large units of protein can be synthesized
D) recycle DNA using thermocyclers B) make many copies of an organism's DNA sequence so
a small number of
organisms will become large enough to be identified
Taq polymerase starts copying at
A) the end of free single-stranded RNA
B) any open point
C) RNA primers attached to the end of the desired gene
D) DNA primers attached to the end of the desired gene. D) DNA primers attached to the
end of the desired gene.
Using PCR, over 1 million copies of DNA can be made from a single strand of DNA overnight.
