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Which of the following is the formula to calculate a manual white cell count?
A. (number of cells counted x dilution x10) number of squares counted
B.(number of cells counted x dilution) / 10 x number of squares
C. (number of cells counted x dilution
D. (number of cells counted x number of squares counted - ANSWER A. (number of
cells counted x dilution x10) number of squares counted
If a WBC count is performed on a 1:100 dilution and the number of cells counted in 8
squares is 50, the total WBC count is?
A. 5,000/uL
B. 6,250/uL
C. 50,000/uL
D. 62,500/uL - ANSWER B. 6,250/uL
A total leukocyte count is 10.0 x 10(3)/uL and 25 NRBCs are seen per 100 leukocytes
on the differential. What is the corrected leukocyte count?
A. 2,000/uL
B. 8,000/uL
C. 10,000/uL
D. 12,000/uL - ANSWER B. 8,000/uL
A flag of immature granulocytes (IG) is reported from a hematology analyzer. The next
step is to perform a(an):
A. Auto Verification
B. Smear review
C. Manual differential
D. Pathology review - ANSWER C. Manual differential. Any automated differential
result flag (to include an IG flag) should be confirmed with a manual differential
In an automated hematology analyzer, the WBC printed result is +++. The next step is
to:
A. Repeat after warming the sample to 37C
B. Make an appropriate dilution of the sample
C. Recalibrate the machine from pooled samples
, D. Request a new sample immediately - ANSWER B. Make an appropriate dilution of
the sample. +++ is an indicator that the WBC count exceeds the upper limit of
established reportable range.
A specimen analyzed on an automated hematology instrument has a platelet count of
19 x 10(3)/uL. the first procedure to follow is:
A. Report the count after the batch run is completed
B. Request a new specimen
C. review the stained blood smear
D. Notify the laboratory manager - ANSWER C. review the stained blood smear. Low
platelet counts should be verified with a slide estimate.
The electrical resistance method of cell counting requires:
A. Equal-sized particles
B. A conductive liquid
C. 2 internal electrodes for current
D. 3 apertures for counting - ANSWER B. A conductive liquid. Coulter principle of
particle counting
An automated hematology analyzer, if the RBC is erroneously increased, how will other
parameters be effected? A. Increased MCHC
B. Increased Hemoglobin
C. Decreased MCH
D. Increased MCV - ANSWER 251C. Decreased MCH. The formula for calculating the
CH uses the RBC.
On the initial start-up of the automated hematology analyzer, one of the controls is
slightly below the range for the MCV. Which of the following is indicated?
A. Call for service
B. Adjust the MCV up slightly
C. Shut down the instrument
D. Repeat the control - ANSWER D. Repeat the control. Repeat of one out of range
control is the first appropriate course of action.
Which of the following will cause erroneous results when using a phase optical system
for enumerating platelets? A. Lysed RBCs
B. Aggretgated platelets
C. Diluted Sample
D. Intact leukocytes - ANSWER B. Aggretgated platelets.