ASCP MB TEST Questions And Answers Verified 100%
Correct
Which termination process in prokaryotes uses a hairpin structure
to slow or stop transcription and is also known as intrinsic
termination?
Rho-dependent termination or Rho-independent termination Why?
Rho-independent termination
Called independent because it does not require any additional
factors or enzymes to initiate termination of transcription. Instead,
the RNA strand will fold onto itself, creating a hairpin structure,
which in turn slows the RNA polymerase from moving along the
strand and ultimately stopping transcription.
How many RNA polymerases do eukaryotes have vs.
prokaryotes?
Eukaryotes = Three RNA Polymerases
1.) RNA Pol I
2.) RNA Pol II
3.) RNA Pol III
Prokaryotes = One RNA Polymerase
,What is the absorptivity constant of DNA?
Of RNA?
DNA = 50ug/mL (for dsDNA)
RNA = 40ug/mL
What is the equation to find the concentration of DNA or RNA
when the absorbance reading and the dilution factor are given?
Concentration = (Absorbance reading) x (absorptivity constant
DNA/RNA) x (dilution factor)
A technologist uses the spectrophotometer to quantify the amount
of DNA extracted from a blood specimen diluted 1:30. The
absorbance reading at 260 nm was found to be 2.545. The
concentration of DNA in this extract is:
Absorbance x Absorptivity constant x Dilution factor
2.545 x 50ug/mL x 30 = 3817.5ug/mL
Mutation in what gene is associated with Fragile X syndrome?
H63D
CFTR
FMR1
F5
FMR1 = Fragile X Mental Retardation 1
*H63D and HFE = Hemochromatosis
CFTR = Cystic Fibrosis Transmembrane Conductance Regulator
F5 = Factor V Leiden (Factor 5 Leiden) Pyrosequencing????
, 1.) A method of DNA sequencing that relies on the detection of
pyrophosphate released upon nucleotide incorporation.
2.) Detects addition of a nucleotide to the end of a synthesized
strand of DNA by production of light
Faster and cheaper than Sanger method.
3.) DNA sequencing technology that is based on the generation
and detection of a pyrophosphate group liberated from a
nucleotide triphosphate
Most common file type for NGS?
FASTQ
(Next most common is SFF)
A mutation in the MTHFR gene causes what vitamin
deficiency(ies)?
MTHFR = Methylenetetrahydrofolate Reductase
Affects the way the body metabolizes folate (folic acid) and other
B vitamins.
**Blood test looks for increased homocysteine in the blood.
Genetic testing can also be preformed. Autosomal recessive.
Briefly describe how end labeling DNA works.
Labeled nucleotides are added at the 3' or 5' end of the DNA via :
T4 DNA polymerase (adds to 3' end)
T4 polynucleotide kinase - T4-PNK (adds to the 5' end) Briefly
describe how random priming of DNA works.
Correct
Which termination process in prokaryotes uses a hairpin structure
to slow or stop transcription and is also known as intrinsic
termination?
Rho-dependent termination or Rho-independent termination Why?
Rho-independent termination
Called independent because it does not require any additional
factors or enzymes to initiate termination of transcription. Instead,
the RNA strand will fold onto itself, creating a hairpin structure,
which in turn slows the RNA polymerase from moving along the
strand and ultimately stopping transcription.
How many RNA polymerases do eukaryotes have vs.
prokaryotes?
Eukaryotes = Three RNA Polymerases
1.) RNA Pol I
2.) RNA Pol II
3.) RNA Pol III
Prokaryotes = One RNA Polymerase
,What is the absorptivity constant of DNA?
Of RNA?
DNA = 50ug/mL (for dsDNA)
RNA = 40ug/mL
What is the equation to find the concentration of DNA or RNA
when the absorbance reading and the dilution factor are given?
Concentration = (Absorbance reading) x (absorptivity constant
DNA/RNA) x (dilution factor)
A technologist uses the spectrophotometer to quantify the amount
of DNA extracted from a blood specimen diluted 1:30. The
absorbance reading at 260 nm was found to be 2.545. The
concentration of DNA in this extract is:
Absorbance x Absorptivity constant x Dilution factor
2.545 x 50ug/mL x 30 = 3817.5ug/mL
Mutation in what gene is associated with Fragile X syndrome?
H63D
CFTR
FMR1
F5
FMR1 = Fragile X Mental Retardation 1
*H63D and HFE = Hemochromatosis
CFTR = Cystic Fibrosis Transmembrane Conductance Regulator
F5 = Factor V Leiden (Factor 5 Leiden) Pyrosequencing????
, 1.) A method of DNA sequencing that relies on the detection of
pyrophosphate released upon nucleotide incorporation.
2.) Detects addition of a nucleotide to the end of a synthesized
strand of DNA by production of light
Faster and cheaper than Sanger method.
3.) DNA sequencing technology that is based on the generation
and detection of a pyrophosphate group liberated from a
nucleotide triphosphate
Most common file type for NGS?
FASTQ
(Next most common is SFF)
A mutation in the MTHFR gene causes what vitamin
deficiency(ies)?
MTHFR = Methylenetetrahydrofolate Reductase
Affects the way the body metabolizes folate (folic acid) and other
B vitamins.
**Blood test looks for increased homocysteine in the blood.
Genetic testing can also be preformed. Autosomal recessive.
Briefly describe how end labeling DNA works.
Labeled nucleotides are added at the 3' or 5' end of the DNA via :
T4 DNA polymerase (adds to 3' end)
T4 polynucleotide kinase - T4-PNK (adds to the 5' end) Briefly
describe how random priming of DNA works.
