BIOS 1705 Lab Quiz 3 – A+ Graded | Real
Questions with Verified Answers
What is DNA polymerase? - ✔✔an enzyme that adds free roaming nucleotides to the
lengthening of the DNA segment
How many cycles to ampligy DNA? - ✔✔25-35
Denature (temp) - ✔✔94 degrees Celsius
Annealing (temp) - ✔✔5 degrees Celsius and below
Extension (temp) - ✔✔72 degrees Celsius
The restriction enzyme that we will use - ✔✔HaeIII
SNP is - ✔✔single nucleotide polymorphism
Its recognition sequence is GGCC, thus it will recognize and cut the "taster" variant of TAS2R38
that encodes for a taste receptor that allows for PTC to be tasted - ✔✔Hae III
If both copies are different - ✔✔Heterozygous
If both copies are the same - ✔✔Homozygous
. Humans inherit two copies, called - ✔✔Alleles
Electrophoresis is? - ✔✔the movement of a charged molecule in an electrical field
, Dna is _____charged? - ✔✔negatively
The agarose gel does what to the movement of the DNA ? - ✔✔slows the movement of the
negatively-charged DNA molecule; the larger the molecule, the harder time it has getting
through the gel. Therefore small molecules migrate relatively quickly to the positive pole and
larger molecules move more slowly
How does bacteria replicate? - ✔✔Asexually by binary fission: ability to incorporate new
genetic material from surroundings
What is transformation? - ✔✔ability to bind naked DNA in the environment and absorb it ?
What type of DNA does E.Coli have? - ✔✔Circular DNA
What are plasmids? - ✔✔Small circular DNA molecules
What does extra-chromosomal mean? - ✔✔they are not a part of the bacterial genome
What is bio luminescent? - ✔✔to glow or produce light
What is pGreen? - ✔✔plasmid which has a gene for ampr and a gene for GFP
Bacteria are grown in? - ✔✔Media
Where are bacteria easily grown? - ✔✔In LB broth and or agar
What is competent? - ✔✔ability to take up the plasmid
What is chemical competancy - ✔✔induced in E.Coli by testing with chloride salts of metal
cation calcium,magnesium and rubidium. Assistance of sudden cycles of heat and cold
Questions with Verified Answers
What is DNA polymerase? - ✔✔an enzyme that adds free roaming nucleotides to the
lengthening of the DNA segment
How many cycles to ampligy DNA? - ✔✔25-35
Denature (temp) - ✔✔94 degrees Celsius
Annealing (temp) - ✔✔5 degrees Celsius and below
Extension (temp) - ✔✔72 degrees Celsius
The restriction enzyme that we will use - ✔✔HaeIII
SNP is - ✔✔single nucleotide polymorphism
Its recognition sequence is GGCC, thus it will recognize and cut the "taster" variant of TAS2R38
that encodes for a taste receptor that allows for PTC to be tasted - ✔✔Hae III
If both copies are different - ✔✔Heterozygous
If both copies are the same - ✔✔Homozygous
. Humans inherit two copies, called - ✔✔Alleles
Electrophoresis is? - ✔✔the movement of a charged molecule in an electrical field
, Dna is _____charged? - ✔✔negatively
The agarose gel does what to the movement of the DNA ? - ✔✔slows the movement of the
negatively-charged DNA molecule; the larger the molecule, the harder time it has getting
through the gel. Therefore small molecules migrate relatively quickly to the positive pole and
larger molecules move more slowly
How does bacteria replicate? - ✔✔Asexually by binary fission: ability to incorporate new
genetic material from surroundings
What is transformation? - ✔✔ability to bind naked DNA in the environment and absorb it ?
What type of DNA does E.Coli have? - ✔✔Circular DNA
What are plasmids? - ✔✔Small circular DNA molecules
What does extra-chromosomal mean? - ✔✔they are not a part of the bacterial genome
What is bio luminescent? - ✔✔to glow or produce light
What is pGreen? - ✔✔plasmid which has a gene for ampr and a gene for GFP
Bacteria are grown in? - ✔✔Media
Where are bacteria easily grown? - ✔✔In LB broth and or agar
What is competent? - ✔✔ability to take up the plasmid
What is chemical competancy - ✔✔induced in E.Coli by testing with chloride salts of metal
cation calcium,magnesium and rubidium. Assistance of sudden cycles of heat and cold
