BIOL 3444 End Term Exam – A+ Paper Full-
Length Q&A Submission
Which of the following would not potentially cause a gram-positive cell to appear gram-
negative after Gram staining? - ✔✔A. counterstaining with crystal violet instead of safranin
Which of the following does not cause the probe to move up and down above the specimen
in atomic force microscopy? - ✔✔D. variations in current
Quantum tunneling of electrons is important in the mechanism underlying which microscope
type? - ✔✔C. STM
Which of the following is the chemical in some bacterial cells' walls that makes them resist
decolorization after acid-fast staining? - ✔✔B. mycolic acid
Gram staining is useful for bacterial identification. Which of the following is another way it is
most useful? - ✔✔C. Knowing Gram staining results is helpful in choosing an effective
antibiotic
Which of the following is not true regarding capsule staining? - ✔✔A. Capsules are typically
stained using basic dyes.
Which of the following most specifically refers to stains with positively charged
chromophores? - ✔✔C. basic dyes
Which of the following most specifically refers to a stain that dyes a specimen rather than the
background? - ✔✔D. positive stain
Which of the following describes the difference between the Ziehl-Neelsen and Kinyoun acid-
fast staining techniques? - ✔✔C. The Ziehl-Neelsen technique uses heat and the Kinyoun
technique does not
, Which of the following described the difference between gram-positive and gram-negative
cells that causes them to stain differently after Gram staining? - ✔✔D. Gram-positive cells
have thicker peptidoglycan layers in their cell walls than do gram-negative cells.
Because endospores are difficult to stain, which of the following is generally needed? - ✔✔C.
Negative staining must be used to stain endospores because they do not absorb stain.
When endospores are stained using the Schaeffer-Fulton method, which of the following
correctly describes how they appear after staining? - ✔✔A. The endospore appears green
within a pink cell, because only the endospore retained the primary stain.
Specimens for TEM are prepared by doing which of the following? - ✔✔C. using an
ultramicrotome to cut very thin sections from samples embedded in plastic resin
In fluorescence, emitted photons have the same amount of energy as absorbed photons. -
✔✔False
A microscope with very high magnification and low resolution would allow you to clearly see
small structures. - ✔✔False
The difference between a simple microscope and a compound microscope is that the latter
only has one lens. - ✔✔False
Flagella staining is very useful for diagnostic purposes in medical settings. - ✔✔False
The transmission electron microscope and scanning electron microscope produce very similar
images, but the former can be show three-dimensional external structures. - ✔✔False
Differential interference contrast microscopy produces three-dimensional images of
specimens. - ✔✔True
Length Q&A Submission
Which of the following would not potentially cause a gram-positive cell to appear gram-
negative after Gram staining? - ✔✔A. counterstaining with crystal violet instead of safranin
Which of the following does not cause the probe to move up and down above the specimen
in atomic force microscopy? - ✔✔D. variations in current
Quantum tunneling of electrons is important in the mechanism underlying which microscope
type? - ✔✔C. STM
Which of the following is the chemical in some bacterial cells' walls that makes them resist
decolorization after acid-fast staining? - ✔✔B. mycolic acid
Gram staining is useful for bacterial identification. Which of the following is another way it is
most useful? - ✔✔C. Knowing Gram staining results is helpful in choosing an effective
antibiotic
Which of the following is not true regarding capsule staining? - ✔✔A. Capsules are typically
stained using basic dyes.
Which of the following most specifically refers to stains with positively charged
chromophores? - ✔✔C. basic dyes
Which of the following most specifically refers to a stain that dyes a specimen rather than the
background? - ✔✔D. positive stain
Which of the following describes the difference between the Ziehl-Neelsen and Kinyoun acid-
fast staining techniques? - ✔✔C. The Ziehl-Neelsen technique uses heat and the Kinyoun
technique does not
, Which of the following described the difference between gram-positive and gram-negative
cells that causes them to stain differently after Gram staining? - ✔✔D. Gram-positive cells
have thicker peptidoglycan layers in their cell walls than do gram-negative cells.
Because endospores are difficult to stain, which of the following is generally needed? - ✔✔C.
Negative staining must be used to stain endospores because they do not absorb stain.
When endospores are stained using the Schaeffer-Fulton method, which of the following
correctly describes how they appear after staining? - ✔✔A. The endospore appears green
within a pink cell, because only the endospore retained the primary stain.
Specimens for TEM are prepared by doing which of the following? - ✔✔C. using an
ultramicrotome to cut very thin sections from samples embedded in plastic resin
In fluorescence, emitted photons have the same amount of energy as absorbed photons. -
✔✔False
A microscope with very high magnification and low resolution would allow you to clearly see
small structures. - ✔✔False
The difference between a simple microscope and a compound microscope is that the latter
only has one lens. - ✔✔False
Flagella staining is very useful for diagnostic purposes in medical settings. - ✔✔False
The transmission electron microscope and scanning electron microscope produce very similar
images, but the former can be show three-dimensional external structures. - ✔✔False
Differential interference contrast microscopy produces three-dimensional images of
specimens. - ✔✔True
