BIOS 1705 Lab Exam Questions With 100%
Verified Answers!!
How does bacteria replicate?
Asexually by binary fission: ability to incorporate new genetic material from surroundings
What is transformation?
ability to bind naked DNA in the environment and absorb it ?
What type of DNA does E.Coli have?
Circular DNA
What are plasmids?
Small circular DNA molecules
What does extra-chromosomal mean?
they are not a part of the bacterial genome
What is bio luminescent?
to glow or produce light
What is pGreen?
plasmid which has a gene for ampr and a gene for GFP
Bacteria are grown in?
Media
Where are bacteria easily grown?
In LB broth and or agar
What is competent?
ability to take up the plasmid
, What is chemical competancy
induced in E.Coli by testing with chloride salts of metal cation calcium,magnesium and
rubidium. Assistance of sudden cycles of heat and cold
What does the heat and cold do in chemical competency?
disrupts the bacterial cell and membrane to create small holes in which DNA enters
What is electroporation?
electric pulses which transiently changes the cell membrane structure and allows foreign DNA
into the cell without killing most of the cells
ampr
commonly used gene when transforming bacteria
Primers are needed for?
amplification of specific parts of DNA
Transformation efficiency
refers to the number of cells that were transformed per micro gram of plasmid DNA
in lab what chemical was used
CaCl2
PCR stands for?
Polymerase chain reaction
What does PCR do?
Amplifies the DNA
What is TAS2R38
Bitter taste receptor (sensitive or insensitive)
Uses of PCR are for what?
Verified Answers!!
How does bacteria replicate?
Asexually by binary fission: ability to incorporate new genetic material from surroundings
What is transformation?
ability to bind naked DNA in the environment and absorb it ?
What type of DNA does E.Coli have?
Circular DNA
What are plasmids?
Small circular DNA molecules
What does extra-chromosomal mean?
they are not a part of the bacterial genome
What is bio luminescent?
to glow or produce light
What is pGreen?
plasmid which has a gene for ampr and a gene for GFP
Bacteria are grown in?
Media
Where are bacteria easily grown?
In LB broth and or agar
What is competent?
ability to take up the plasmid
, What is chemical competancy
induced in E.Coli by testing with chloride salts of metal cation calcium,magnesium and
rubidium. Assistance of sudden cycles of heat and cold
What does the heat and cold do in chemical competency?
disrupts the bacterial cell and membrane to create small holes in which DNA enters
What is electroporation?
electric pulses which transiently changes the cell membrane structure and allows foreign DNA
into the cell without killing most of the cells
ampr
commonly used gene when transforming bacteria
Primers are needed for?
amplification of specific parts of DNA
Transformation efficiency
refers to the number of cells that were transformed per micro gram of plasmid DNA
in lab what chemical was used
CaCl2
PCR stands for?
Polymerase chain reaction
What does PCR do?
Amplifies the DNA
What is TAS2R38
Bitter taste receptor (sensitive or insensitive)
Uses of PCR are for what?
