BIOS 1705 Exam 2 Questions With 100%
Verified Answers!!
At which temperature would do you think Taq would be most active at? Why?
Taq would work best at 65 degrees celsius because the enzymes have evolved to function at this
temperature because this temperature is the state that it is normally in.
Propose two ways in which you could measure the activity of Taq
By measuring the disappearance of the substrate or the appearance of its product
Once you have determined the best (optimum) temperature for Taq to work at, you next
try to test a molecule that you think might act as an inhibitor of Taq. When you add the
molecule to a reaction you see less activity. Adding more substrate with the inhibitor
prevented the inhibitor from decreasing Taq activity. What type of inhibitor is the
molecule you tested?
This would be a competitive inhibitor
What are the stages of respiration?
Glycolysis, Acetyl-CoA synthesis, Citric Acid Cycle, oxidative phosphorylation
What substances are used in glycolysis?
glucose and oxygen
What substances are made in glycolysis?
ATP and CO2
Why can oxygen consumption be used as a measure of cellular respiration?
Oxygen is the final electron acceptor in the electron transport chain
How does temperature affect enzyme activity?
Enzymes work faster at high temperatures and slower at lower temperatures
Verified Answers!!
At which temperature would do you think Taq would be most active at? Why?
Taq would work best at 65 degrees celsius because the enzymes have evolved to function at this
temperature because this temperature is the state that it is normally in.
Propose two ways in which you could measure the activity of Taq
By measuring the disappearance of the substrate or the appearance of its product
Once you have determined the best (optimum) temperature for Taq to work at, you next
try to test a molecule that you think might act as an inhibitor of Taq. When you add the
molecule to a reaction you see less activity. Adding more substrate with the inhibitor
prevented the inhibitor from decreasing Taq activity. What type of inhibitor is the
molecule you tested?
This would be a competitive inhibitor
What are the stages of respiration?
Glycolysis, Acetyl-CoA synthesis, Citric Acid Cycle, oxidative phosphorylation
What substances are used in glycolysis?
glucose and oxygen
What substances are made in glycolysis?
ATP and CO2
Why can oxygen consumption be used as a measure of cellular respiration?
Oxygen is the final electron acceptor in the electron transport chain
How does temperature affect enzyme activity?
Enzymes work faster at high temperatures and slower at lower temperatures
