lOMoAR cPSD| 26712239
BIO 2280 - MICRO BIOLOGY COMPLETE SET
QUESTIONS WITH ANSWERS | VERIFIED 100%
CORRECT
I. Generation time
A. How can scientists measure the growth rate of a microbial population?
1. By calculating its generation time
B. Do all microbes have the same generation time?
1. No, different types of microbes will have different generation times.
II. Microbial growth curves
A. What is a closed system in a lab?
1. When the cells that are grown are completely enclosed and nothing can
come in and out?
B. What will happen to microbial growth in a closed system?
1. Eventually all microorganisms would die due to the limitations of the
amount of nutrients that are present.
C. What happens during the lag phase in the microbial growth curve?
1. Cells from a pure colony begin to make the enzymes that will be required
for cell growth
D. What happens during the log or exponential phase in the microbial growth curve?
1. Cell division occurs at a rapid pace
E. What happens during the stationary phase in the microbial growth curve?
, lOMoAR cPSD| 26712239
1. Because the nutrients are limited, the cells eventually stop growing and
some microbes start dying
F. What happens during the decline or death phase in the microbial growth curve?
1. The viable or living cells die
G. Why does the death phase occur relatively slowly?
, lOMoAR cPSD| 26712239
1.
Because when some microbes die, they release their contents which are
able to feed other microbes, this is why it's not a steep curve, but rather
appears to be more linear.
H. During what phase of the microbial growth curve is the rate of cell production
and cell death approximately the same?
1. During the stationary phase
I. What is produced in the late log phase of the bacterial growth curve?
1. Secondary metabolites
J. What is produced in the early log phase of the bacterial growth curve?
1. Primary metabolites
III. Direct cell counts
A. When would you use direct cell counting?
1. When you are trying to determine the total number of cells in a culture
B. What is a caveat to direct cell counting?
1. It includes viable and dead cells, so if you’re trying to only count viable
cells, this method may no be the best approach
C. Name an example of a method that would be used to determine the number of
cells in a culture:
1. Microscopic cell counting
BIO 2280 - MICRO BIOLOGY COMPLETE SET
QUESTIONS WITH ANSWERS | VERIFIED 100%
CORRECT
I. Generation time
A. How can scientists measure the growth rate of a microbial population?
1. By calculating its generation time
B. Do all microbes have the same generation time?
1. No, different types of microbes will have different generation times.
II. Microbial growth curves
A. What is a closed system in a lab?
1. When the cells that are grown are completely enclosed and nothing can
come in and out?
B. What will happen to microbial growth in a closed system?
1. Eventually all microorganisms would die due to the limitations of the
amount of nutrients that are present.
C. What happens during the lag phase in the microbial growth curve?
1. Cells from a pure colony begin to make the enzymes that will be required
for cell growth
D. What happens during the log or exponential phase in the microbial growth curve?
1. Cell division occurs at a rapid pace
E. What happens during the stationary phase in the microbial growth curve?
, lOMoAR cPSD| 26712239
1. Because the nutrients are limited, the cells eventually stop growing and
some microbes start dying
F. What happens during the decline or death phase in the microbial growth curve?
1. The viable or living cells die
G. Why does the death phase occur relatively slowly?
, lOMoAR cPSD| 26712239
1.
Because when some microbes die, they release their contents which are
able to feed other microbes, this is why it's not a steep curve, but rather
appears to be more linear.
H. During what phase of the microbial growth curve is the rate of cell production
and cell death approximately the same?
1. During the stationary phase
I. What is produced in the late log phase of the bacterial growth curve?
1. Secondary metabolites
J. What is produced in the early log phase of the bacterial growth curve?
1. Primary metabolites
III. Direct cell counts
A. When would you use direct cell counting?
1. When you are trying to determine the total number of cells in a culture
B. What is a caveat to direct cell counting?
1. It includes viable and dead cells, so if you’re trying to only count viable
cells, this method may no be the best approach
C. Name an example of a method that would be used to determine the number of
cells in a culture:
1. Microscopic cell counting
