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CLINICAL CHEMISTRY I ASCP
REVIEW 2025 BRAND NEW ACTUAL
EXAM WITH ANSWERS.
Which of the following lamps provides a continuous spectrum of
radiant energy in the visible, near IR, and near UV regions of the
spectrum?
A. Tungsten-filament
B. Hydrogen
C. Deuterium
D. Mercury vapor - correct answer -A most common light source
for photometry in the visible region. continuous spectrum (360-
800 nm). near IR and UV.
Hydrogen and Deuterium (200-375 nm)
Mercury vapor- not a continuous spectrum, emitting radiation at
specific wavelengths
Which of the following isolates light
within a narrow region of the spectrum?
A. Photomultiplier tube
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B. Monochromator
C. Photovoltaic cell
D. Detector - correct answer -B Use for selecting a narrow band
wavelengths from a continuous spectrum. Three kinds (prisms,
filters, diffraction gratings.
Which of the following is not descriptive of a photomultiplier tube?
A. Emits electrons proportionally to
initial light absorbed
B. Must be shielded from stray light
C. Cannot be used with a chopper
D. Amplifies the initial signal received - correct answer -C
PMT- responds to the radiant energy light it absorbs by emitting
electrons in a proportional amount to the initial light absorbed.
Which of the following is false about
a photomultiplier tube?
A. Converts radiant energy (light)
to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
D. Is composed of an iron plate and
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a layer of selenium - correct answer -D is part of a barrier layer
cell
PMT- 1. light to electricity
2. Amplification
5. Which type of photodetector employs a linear arrangement that
allows it to respond to a specific wavelength resulting in complete
UV/visible spectrum analysis?
A. Photomultiplier tube
B. Phototube
C. Barrier layer cell
D. Photodiode array - correct answer -D
256-2048 arranged in a linear fashion
Respond to a specific wavelength that results in a continuous UV/
visible spectrum.
When performing spectrophotometer
quality assurance checks, what is the
holmium oxide glass filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
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D. Wavelength accuracy - correct answer -D
In spectrophotometric analysis, what is the purpose of the reagent
blank?
A. Correct for interfering chromogens
B. Correct for lipemia
C. Correct for protein
D. Correct for color contribution of the reagents - correct answer -
D
In regard to bichromatic analysis, which of the following is false?
A. Absorbance is measured at the
spectral absorbance peak for a blank
and the sample using the same
wavelength.
B. Eliminates background interferences
C. Sample concentration determined
from difference in two measured
absorbances
D. Functions as a reference blank for
each sample - correct answer -A
CLINICAL CHEMISTRY I ASCP
REVIEW 2025 BRAND NEW ACTUAL
EXAM WITH ANSWERS.
Which of the following lamps provides a continuous spectrum of
radiant energy in the visible, near IR, and near UV regions of the
spectrum?
A. Tungsten-filament
B. Hydrogen
C. Deuterium
D. Mercury vapor - correct answer -A most common light source
for photometry in the visible region. continuous spectrum (360-
800 nm). near IR and UV.
Hydrogen and Deuterium (200-375 nm)
Mercury vapor- not a continuous spectrum, emitting radiation at
specific wavelengths
Which of the following isolates light
within a narrow region of the spectrum?
A. Photomultiplier tube
, Page | 2
B. Monochromator
C. Photovoltaic cell
D. Detector - correct answer -B Use for selecting a narrow band
wavelengths from a continuous spectrum. Three kinds (prisms,
filters, diffraction gratings.
Which of the following is not descriptive of a photomultiplier tube?
A. Emits electrons proportionally to
initial light absorbed
B. Must be shielded from stray light
C. Cannot be used with a chopper
D. Amplifies the initial signal received - correct answer -C
PMT- responds to the radiant energy light it absorbs by emitting
electrons in a proportional amount to the initial light absorbed.
Which of the following is false about
a photomultiplier tube?
A. Converts radiant energy (light)
to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
D. Is composed of an iron plate and
, Page | 3
a layer of selenium - correct answer -D is part of a barrier layer
cell
PMT- 1. light to electricity
2. Amplification
5. Which type of photodetector employs a linear arrangement that
allows it to respond to a specific wavelength resulting in complete
UV/visible spectrum analysis?
A. Photomultiplier tube
B. Phototube
C. Barrier layer cell
D. Photodiode array - correct answer -D
256-2048 arranged in a linear fashion
Respond to a specific wavelength that results in a continuous UV/
visible spectrum.
When performing spectrophotometer
quality assurance checks, what is the
holmium oxide glass filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
, Page | 4
D. Wavelength accuracy - correct answer -D
In spectrophotometric analysis, what is the purpose of the reagent
blank?
A. Correct for interfering chromogens
B. Correct for lipemia
C. Correct for protein
D. Correct for color contribution of the reagents - correct answer -
D
In regard to bichromatic analysis, which of the following is false?
A. Absorbance is measured at the
spectral absorbance peak for a blank
and the sample using the same
wavelength.
B. Eliminates background interferences
C. Sample concentration determined
from difference in two measured
absorbances
D. Functions as a reference blank for
each sample - correct answer -A
