1 Exampromax - Stuvia US 2025/2026
BIOL 3444 EXAM 1 Questions and Answers
(100% Correct Answers) Already Graded A+
Which term is used to refer to a wave's rate of vibration?
Ans: B. frequency
On the figure, which letter represents a refracted ray of light?
Ans: C (BUT D on test)
On the electromagnetic spectrum, which type of wave has the shortest
wavelength?
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Ans: A. cosmic ray
Exampromax - Stuvia US
Dyes are often used during microscopy to increase which of the following?
Ans: A. contrast
Which of the following was the first to use the term "cells"?
Ans: A. Robert Hooke
Which of the following is the typical magnification of an object viewed
using a 10× ocular lens and a 100× objective lens on a brightfield
microscope?
Ans: D. 1000×
Most modern microscopes have two eyepieces, meaning that they are
which of the following?
Ans: A. binocular
An annular stop is used for which type of microscopy?
Ans: D. phase contrast
Which of the following is the specific name for a stain that colors the
background but not the specimen?
, 2 Exampromax - Stuvia US 2025/2026
Ans: D. negative stain
Which type of microscopy would work best for viewing internal cell
structures?
Ans: D. transmission electron microscopy
The Gram-staining procedure is best described as which of the following?
Ans: B. differential staining
When there is a substantial difference in refractive index between two
materials, which of the following describes what will happen to light passing
from one material to the other?
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Ans: B. It will undergo a large amount of refraction.
Exampromax - Stuvia US
In microscopy, the focal length refers to which of the following?
Ans: D. the distance to the image point (at which all light entering a lens is
parallel)
Which of the following are pigments used in microscopy to absorb and
reflect light?
Ans: B. chromophores
Negative staining is the standard approach used to visualize which
structure(s)?
Ans: A. capsules
Which is a standard mordant used in flagella staining?
Ans: D. tannic acid
Which of the following pairs are common acid-fast staining techniques?
Ans: C. Ziehl-Neelsen and Kinyoun
Which is the primary stain used in the Ziehl-Neelsen technique?
Ans: B. carbolfuchsin
, 3 Exampromax - Stuvia US 2025/2026
Which is the correct order of steps in Gram staining?
Ans: B. crystal violet stain, Gram's iodine mordant, ethanol decolorization,
safranin counterstain
It is important to fix bacterial smears on a slide for which of the following
reasons?
Ans: A. to attach them to the slide
Which of the following is not commonly used for chemical fixation?
Ans: D. iodine
Which of the following is not involved in preparing a bacterial slide for
© 2025 Assignment Expert
Gram staining?
Exampromax - Stuvia US
Ans: D. preparing a wet mount
Which of the following most specifically refers to stains with positively
charged chromophores?
Ans: C. basic dyes
Which of the following most specifically refers to a stain that dyes a
specimen rather than the background?
Ans: D. positive stain
Which of the following describes the difference between the Ziehl-Neelsen
and Kinyoun acid-fast staining techniques?
Ans: C. The Ziehl-Neelsen technique uses heat and the Kinyoun technique does
not
Which of the following described the difference between gram-positive and
gram-negative cells that causes them to stain differently after Gram staining?
Ans: D. Gram-positive cells have thicker peptidoglycan layers in their cell walls
than do gram-negative cells.
Which of the following would not potentially cause a gram-positive cell to
appear gram-negative after Gram staining?
BIOL 3444 EXAM 1 Questions and Answers
(100% Correct Answers) Already Graded A+
Which term is used to refer to a wave's rate of vibration?
Ans: B. frequency
On the figure, which letter represents a refracted ray of light?
Ans: C (BUT D on test)
On the electromagnetic spectrum, which type of wave has the shortest
wavelength?
© 2025 Assignment Expert
Ans: A. cosmic ray
Exampromax - Stuvia US
Dyes are often used during microscopy to increase which of the following?
Ans: A. contrast
Which of the following was the first to use the term "cells"?
Ans: A. Robert Hooke
Which of the following is the typical magnification of an object viewed
using a 10× ocular lens and a 100× objective lens on a brightfield
microscope?
Ans: D. 1000×
Most modern microscopes have two eyepieces, meaning that they are
which of the following?
Ans: A. binocular
An annular stop is used for which type of microscopy?
Ans: D. phase contrast
Which of the following is the specific name for a stain that colors the
background but not the specimen?
, 2 Exampromax - Stuvia US 2025/2026
Ans: D. negative stain
Which type of microscopy would work best for viewing internal cell
structures?
Ans: D. transmission electron microscopy
The Gram-staining procedure is best described as which of the following?
Ans: B. differential staining
When there is a substantial difference in refractive index between two
materials, which of the following describes what will happen to light passing
from one material to the other?
© 2025 Assignment Expert
Ans: B. It will undergo a large amount of refraction.
Exampromax - Stuvia US
In microscopy, the focal length refers to which of the following?
Ans: D. the distance to the image point (at which all light entering a lens is
parallel)
Which of the following are pigments used in microscopy to absorb and
reflect light?
Ans: B. chromophores
Negative staining is the standard approach used to visualize which
structure(s)?
Ans: A. capsules
Which is a standard mordant used in flagella staining?
Ans: D. tannic acid
Which of the following pairs are common acid-fast staining techniques?
Ans: C. Ziehl-Neelsen and Kinyoun
Which is the primary stain used in the Ziehl-Neelsen technique?
Ans: B. carbolfuchsin
, 3 Exampromax - Stuvia US 2025/2026
Which is the correct order of steps in Gram staining?
Ans: B. crystal violet stain, Gram's iodine mordant, ethanol decolorization,
safranin counterstain
It is important to fix bacterial smears on a slide for which of the following
reasons?
Ans: A. to attach them to the slide
Which of the following is not commonly used for chemical fixation?
Ans: D. iodine
Which of the following is not involved in preparing a bacterial slide for
© 2025 Assignment Expert
Gram staining?
Exampromax - Stuvia US
Ans: D. preparing a wet mount
Which of the following most specifically refers to stains with positively
charged chromophores?
Ans: C. basic dyes
Which of the following most specifically refers to a stain that dyes a
specimen rather than the background?
Ans: D. positive stain
Which of the following describes the difference between the Ziehl-Neelsen
and Kinyoun acid-fast staining techniques?
Ans: C. The Ziehl-Neelsen technique uses heat and the Kinyoun technique does
not
Which of the following described the difference between gram-positive and
gram-negative cells that causes them to stain differently after Gram staining?
Ans: D. Gram-positive cells have thicker peptidoglycan layers in their cell walls
than do gram-negative cells.
Which of the following would not potentially cause a gram-positive cell to
appear gram-negative after Gram staining?
