1 Exampromax - Stuvia US 2025/2026
BIOS 1705 Lab Quiz 2 Questions and Answers
(100% Correct Answers) Already Graded A+
At which temperature would do you think Taq would be most active at?
Why?
Ans: Taq would work best at 65 degrees celsius because the enzymes have
evolved to function at this temperature because this temperature is the state that it
is normally in.
Propose two ways in which you could measure the activity of Taq
Ans: By measuring the disappearance of the substrate or the appearance of its
product
© 2025 Assignment Expert
Once you have determined the best (optimum) temperature for Taq to work
Exampromax - Stuvia US
at, you next try to test a molecule that you think might act as an inhibitor of
Taq. When you add the molecule to a reaction you see less activity. Adding
more substrate with the inhibitor prevented the inhibitor from decreasing
Taq activity. What type of inhibitor is the molecule you tested?
Ans: This would be a competitive inhibitor
What are the stages of respiration?
Ans: Glycolysis, Acetyl-CoA synthesis, Citric Acid Cycle, oxidative
phosphorylation
What substances are used in glycolysis?
Ans: glucose and oxygen
What substances are made in glycolysis?
Ans: ATP and CO2
Why can oxygen consumption be used as a measure of cellular respiration?
Ans: Oxygen is the final electron acceptor in the electron transport chain
How does temperature affect enzyme activity?
BIOS 1705 Lab Quiz 2 Questions and Answers
(100% Correct Answers) Already Graded A+
At which temperature would do you think Taq would be most active at?
Why?
Ans: Taq would work best at 65 degrees celsius because the enzymes have
evolved to function at this temperature because this temperature is the state that it
is normally in.
Propose two ways in which you could measure the activity of Taq
Ans: By measuring the disappearance of the substrate or the appearance of its
product
© 2025 Assignment Expert
Once you have determined the best (optimum) temperature for Taq to work
Exampromax - Stuvia US
at, you next try to test a molecule that you think might act as an inhibitor of
Taq. When you add the molecule to a reaction you see less activity. Adding
more substrate with the inhibitor prevented the inhibitor from decreasing
Taq activity. What type of inhibitor is the molecule you tested?
Ans: This would be a competitive inhibitor
What are the stages of respiration?
Ans: Glycolysis, Acetyl-CoA synthesis, Citric Acid Cycle, oxidative
phosphorylation
What substances are used in glycolysis?
Ans: glucose and oxygen
What substances are made in glycolysis?
Ans: ATP and CO2
Why can oxygen consumption be used as a measure of cellular respiration?
Ans: Oxygen is the final electron acceptor in the electron transport chain
How does temperature affect enzyme activity?
