1 Exampromax - Stuvia US 2025/2026
Bios 1705 Lab Quiz 3 Questions and Answers
(100% Correct Answers) Already Graded A+
How does bacteria replicate?
Ans: Asexually by binary fission: ability to incorporate new genetic material
from surroundings
What is transformation?
Ans: ability to bind naked DNA in the environment and absorb it ?
What type of DNA does E.Coli have?
© 2025 Assignment Expert
Ans: Circular DNA
Exampromax - Stuvia US
What are plasmids?
Ans: Small circular DNA molecules
What does extra-chromosomal mean?
Ans: they are not a part of the bacterial genome
What is bio luminescent?
Ans: to glow or produce light
What is pGreen?
Ans: plasmid which has a gene for ampr and a gene for GFP
Bacteria are grown in?
Ans: Media
Where are bacteria easily grown?
Ans: In LB broth and or agar
What is competent?
Ans: ability to take up the plasmid
, 2 Exampromax - Stuvia US 2025/2026
What is chemical competancy
Ans: induced in E.Coli by testing with chloride salts of metal cation
calcium,magnesium and rubidium. Assistance of sudden cycles of heat and cold
What does the heat and cold do in chemical competency?
Ans: disrupts the bacterial cell and membrane to create small holes in which
DNA enters
What is electroporation?
Ans: electric pulses which transiently changes the cell membrane structure and
allows foreign DNA into the cell without killing most of the cells
ampr
© 2025 Assignment Expert
Ans: commonly used gene when transforming bacteria
Exampromax - Stuvia US
Primers are needed for?
Ans: amplification of specific parts of DNA
Transformation efficiency
Ans: refers to the number of cells that were transformed per micro gram of
plasmid DNA
in lab what chemical was used
Ans: CaCl2
PCR stands for?
Ans: Polymerase chain reaction
What does PCR do?
Ans: Amplifies the DNA
What is TAS2R38
Ans: Bitter taste receptor (sensitive or insensitive)
Uses of PCR are for what?
Bios 1705 Lab Quiz 3 Questions and Answers
(100% Correct Answers) Already Graded A+
How does bacteria replicate?
Ans: Asexually by binary fission: ability to incorporate new genetic material
from surroundings
What is transformation?
Ans: ability to bind naked DNA in the environment and absorb it ?
What type of DNA does E.Coli have?
© 2025 Assignment Expert
Ans: Circular DNA
Exampromax - Stuvia US
What are plasmids?
Ans: Small circular DNA molecules
What does extra-chromosomal mean?
Ans: they are not a part of the bacterial genome
What is bio luminescent?
Ans: to glow or produce light
What is pGreen?
Ans: plasmid which has a gene for ampr and a gene for GFP
Bacteria are grown in?
Ans: Media
Where are bacteria easily grown?
Ans: In LB broth and or agar
What is competent?
Ans: ability to take up the plasmid
, 2 Exampromax - Stuvia US 2025/2026
What is chemical competancy
Ans: induced in E.Coli by testing with chloride salts of metal cation
calcium,magnesium and rubidium. Assistance of sudden cycles of heat and cold
What does the heat and cold do in chemical competency?
Ans: disrupts the bacterial cell and membrane to create small holes in which
DNA enters
What is electroporation?
Ans: electric pulses which transiently changes the cell membrane structure and
allows foreign DNA into the cell without killing most of the cells
ampr
© 2025 Assignment Expert
Ans: commonly used gene when transforming bacteria
Exampromax - Stuvia US
Primers are needed for?
Ans: amplification of specific parts of DNA
Transformation efficiency
Ans: refers to the number of cells that were transformed per micro gram of
plasmid DNA
in lab what chemical was used
Ans: CaCl2
PCR stands for?
Ans: Polymerase chain reaction
What does PCR do?
Ans: Amplifies the DNA
What is TAS2R38
Ans: Bitter taste receptor (sensitive or insensitive)
Uses of PCR are for what?
