Science Biology Microbiology
UTA Microbiology Exam 3 2025 EXAM
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Microbiology Chapter 6 test bank Purdue BIOL 221 Spring 2022 Exam 1... TDLR Sanitation, Dis
Teacher 92 terms 36 terms 36 terms
wanjagrace701 Preview Preview TutorMolly
1. Growth of cell size
2. Replication of DNA
4 Basic Steps of binary fission
3. Division of cytoplasm
4. Septum formation & cell division
Z Ring Assembly FtsZ assembles Z ring to form divisome
Generation Time time it takes to double the population
Lag Phase Cells adjust to culture medium; no change in population
What factors determine the Genetic makeup, media composition, initial inoculum size
duration of the lag phase?
During which phase of the Log phase
growth curve is it most
appropriate for the use of
antibiotics?
Following the death phase of Persisters
the growth curve, ____ cells are
considered the surviving cells &
are considered clinically
important due to their
association with chronic
infections, such as Tuberculosis.
Log Phase Binary fission occurs; cell replication > cell death
Stationary Phase Resources become depleted; cell respiration=cell death
Death Phase Endospores can form; cell replication < cell death
Close System Cultures Have finite resources
, -Nutrients and air are replenished
Open System Cultures -Dead cells and waste are removed
-Beneficial for industrial microbiology
Microscopic Cell Count Cells are counted under a microscope using a laser etched slide
In using the direct microscopic False
cell count method, you will be
able to distinguish between live
vs dead cells.
Fluorescence Staining Cells are counted under a microscope or flow cytometer
In fluorescent staining, the end Green
product results in live cells
staining ___________?
In fluorescent staining, the end Red
product results in dead cells
staining ___________?
Coulter counting Detects electrical resistance change due to cell density
Viable Cell Counts Samples are diluted and grown on solid media
What type of cells are counted Living
when using the viable plate
count method?
Countable Range 30-300 CFU/ml
-Measured with spectrophotometer
Optical Density
-Light is passed thru culture and measured on other side
Which method of measuring Optical density
growth is considered an indirect
approach?
Which method of measuring Fluorescent stain
growth can be used on living or
dead cells?
How is the pour plate technique The sample is mixed with a warm agar ranging from 45-50 C,
set up? poured onto sterile plated and swirled to mix
How is the spread plate The sample is poured onto a solid medium and spread using a L
technique set up? spreader
What cell counting method is Membrane filtration
used for counting colonies from
drinking water?
What method of measuring MPN (most probable number)
growth is used in food/water
testing?
1. Attachment of planktonic cells
2. Cells become sessile
Steps of Biofilm Formation 3. Growth & Division on substrate
4. Production of extracellular polymeric substance
5. Attachment of secondary colonizers & dispersion
Quorum Sensing Cell to cell communication regarding cell density
UTA Microbiology Exam 3 2025 EXAM
QUESTIONS WITH ACCURATE VERIFIED
ANSWERS (ACCURATE SOLUTIONS) A+
PASSED!!v
Save
Students also studied
Flashcard sets Study guides
Microbiology Chapter 6 test bank Purdue BIOL 221 Spring 2022 Exam 1... TDLR Sanitation, Dis
Teacher 92 terms 36 terms 36 terms
wanjagrace701 Preview Preview TutorMolly
1. Growth of cell size
2. Replication of DNA
4 Basic Steps of binary fission
3. Division of cytoplasm
4. Septum formation & cell division
Z Ring Assembly FtsZ assembles Z ring to form divisome
Generation Time time it takes to double the population
Lag Phase Cells adjust to culture medium; no change in population
What factors determine the Genetic makeup, media composition, initial inoculum size
duration of the lag phase?
During which phase of the Log phase
growth curve is it most
appropriate for the use of
antibiotics?
Following the death phase of Persisters
the growth curve, ____ cells are
considered the surviving cells &
are considered clinically
important due to their
association with chronic
infections, such as Tuberculosis.
Log Phase Binary fission occurs; cell replication > cell death
Stationary Phase Resources become depleted; cell respiration=cell death
Death Phase Endospores can form; cell replication < cell death
Close System Cultures Have finite resources
, -Nutrients and air are replenished
Open System Cultures -Dead cells and waste are removed
-Beneficial for industrial microbiology
Microscopic Cell Count Cells are counted under a microscope using a laser etched slide
In using the direct microscopic False
cell count method, you will be
able to distinguish between live
vs dead cells.
Fluorescence Staining Cells are counted under a microscope or flow cytometer
In fluorescent staining, the end Green
product results in live cells
staining ___________?
In fluorescent staining, the end Red
product results in dead cells
staining ___________?
Coulter counting Detects electrical resistance change due to cell density
Viable Cell Counts Samples are diluted and grown on solid media
What type of cells are counted Living
when using the viable plate
count method?
Countable Range 30-300 CFU/ml
-Measured with spectrophotometer
Optical Density
-Light is passed thru culture and measured on other side
Which method of measuring Optical density
growth is considered an indirect
approach?
Which method of measuring Fluorescent stain
growth can be used on living or
dead cells?
How is the pour plate technique The sample is mixed with a warm agar ranging from 45-50 C,
set up? poured onto sterile plated and swirled to mix
How is the spread plate The sample is poured onto a solid medium and spread using a L
technique set up? spreader
What cell counting method is Membrane filtration
used for counting colonies from
drinking water?
What method of measuring MPN (most probable number)
growth is used in food/water
testing?
1. Attachment of planktonic cells
2. Cells become sessile
Steps of Biofilm Formation 3. Growth & Division on substrate
4. Production of extracellular polymeric substance
5. Attachment of secondary colonizers & dispersion
Quorum Sensing Cell to cell communication regarding cell density
